Wyniki wyszukiwania

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Rapid and simultaneous screening of 47,XXY and AZF microdeletions by quadruplex real-time polymerase chain reaction

100%

Zhou Y., Ge Y., Xiao L., Guo Q.

Reproductive Biology

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2015

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tom 15

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nr 2

2

The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil

88%

Xu J., Xu P., Li Z., Xiao L., Yang Z.

Cellular and Molecular Biology Letters

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2013

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tom 18

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nr 4

The aim of malignant glioma treatment is to inhibit tumor cell proliferation and induce tumor cell apoptosis. Remifentanil is a clinical anesthetic drug that can activate the N-methyl-D-aspartate (NMDA) receptor. NMDA receptor signaling activates glycogen synthase kinase-3β (GSK-3β). Discovered some 32 years ago, GSK-3β was only recently considered as a therapeutic target in cancer treatment. The purpose of this study was to assess whether remifentanil can induce the apoptosis of C6 cells through GSK-3β activation. 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) was used to detect cell viability. Hoechst 33342 staining and flow cytometry were used to detect cell apoptosis. The effect of GSK-3β activation was detected using a GSK-3β activation assay kit and 4-benzyl-2-methyl-1,2,4-thiadiazolidine-3,5-dione (TDZD-8), a potent and selective small molecule inhibitor of GSK-3β. The MTT assay indicated that remifentanil induced C6 cell death in a concentration- and time-dependent manner. Hoechst 33342 staining and flow cytometry showed that remifentanil significantly induced C6 cell apoptosis. The measurement of GSK-3β activation showed that remifentanil increased the cellular level of GSK-3β. All of these toxic effects can be attenuated by treatment with TDZD-8. These results suggest that remifentanil is able to induce C6 cell apoptosis through GSK-3β activation, which provides a basis for its potential use in the treatment of malignant gliomas.

3

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Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein

64%

Wu X., Xiao L., Peng B., Wang Y., Yang Z., Yao X., Hu L., Lin X.

Polish Journal of Veterinary Sciences

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2016

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tom 19

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nr 1

African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.

4

The diversity of the endobiotic bacterial communities in the four jellyfish species

64%

Liu Q., Li X., Hong J., Jlang G., Llang H., Liu W., Xu Z., Zhang J., Wang W., Xiao L.

Polish Journal of Microbiology

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2019

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tom 68

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nr 4

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Polish Journal of Microbiology

1 Polish Journal of Veterinary Sciences

1 Reproductive Biology

4 Xiao L.

2 Yang Z.

1 Ge Y.

1 Guo Q.

1 Hong J.

1 Hu L.

1 Jlang G.

1 Li X.

1 Li Z.

1 Lin X.

1 Liu Q.

1 Liu W.

1 Llang H.

1 Peng B.

1 Wang W.

1 Wang Y.

1 Wu X.

1 Xu J.

1 Xu P.

1 Xu Z.

1 Yao X.

1 Zhang J.

1 Zhou Y.

1 2019

1 2016

1 2015

1 2013

Rapid and simultaneous screening of 47,XXY and AZF microdeletions by quadruplex real-time polymerase chain reaction

The role of glycogen synthase kinase-3β in glioma cell apoptosis induced by remifentanil

Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein

The diversity of the endobiotic bacterial communities in the four jellyfish species