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The aim of the study was to determine the frequency of occurrence of environmental mastitis in cows and to identify its main environmental pathogens. A total of 1929 milk samples were examined aseptically taken from 652 cows suffering from mastitis, from 9 dairy herds. Milk samples were plated as soon as possible on blood agar, Mac Conkey agar, Sabouraud agar and aesculin-talium acetate crystal violet agar. Bacteria and yeasts were identified by standard methods and using the API system. From these, 327 environmental infections were identified. The most frequently isolated environmental pathogens were environmental streptococci (51.07%), fungi (20.48%), coliforms (19.87%) and algae (2.44%). The mean proportion of cows affected by environmental mastitis was 9.8 percent. The mean percentage of quarters with environmental mastitis was 3.35%, with a mean of 1.3 quarter cases per cow.
Coryneform bacteria occur in different environments: soil, water, plants, organisms, humans and animals. Many of them are a part of the normal flora of human and animals while others are opportunistic bacteria. Coryneform bacteria are not new microbes, but their role in infections of humans and animals is still underestimated. The genus of Corynebacterium comprises more than 70 species, including 43 of clinical significance. Among the known Corynebacterium species are distinguished: human and animal pathogens, commensal colonizing of the skin and mucous membranes of the respiratory system and genital tract, as well as plant pathogens and saprophytes living in an abiotic environment (plant debris, soil, water). Predisposing factors for Corynebacterium spp infection include: immunosuppression, long-lasting and broad-band spectrum antibiotics, steroids, an age of over 65-years-of-age, ischemic heart disease, kidney failure, respiratory failure, diabetes, cancer, multi-organ injuries, infections by HIV and CMV viruses, prematurity, tears of the skin and mucous membranes, and invasive medical procedures. The paper presents the occurrence and virulence factors of Corynebacterium spp. Infections caused by Corynebacterium spp. Their resistance to antibiotics are also described.
The purpose of this study was to investigate the influence of dairy herd type (small-type farms, big dairy herds), milking system (milking parlor, milking bucket, milking pipeline) and maintenance system of cows (free stalls, tie stalls) on the presence of yeast mastitis in cows. The studies included the cases of mastitis mycotica diagnosed in the Department of Animal Hygiene and Environment in the years 1996-2009. In total, 1982 cows of Polish Holstein-Friesian breed, 60 cows of Simental breed, and 177 cows of the Montbeliard breed were examined. Animals belonged to 34 dairy herds in various regions of the Poland. The percentage of cows and quarters with yeast mastitis in small-type farms was (respectively) 3.57%, and 1.05% while in the large dairy herds only 0.65% and 0.18%. Differences were statistically significant (p ≤ 0.05). An average 3.74% of cows and 1.11% of the quarters showed mastitis mycotica in farms with a tie stall maintenance system, while in the free stall maintenance system (respectively) 0.82% and 0.22%. Differences were statistically significant. The occurrence of yeast mastitis due to a milking system was the following (cows and quarters): 5.47% and 1.66 for farms using milking bucket, 2.21% and 0.69% in dairy farms with milking pipeline and 0.71% and 0.2% in the dairy farms with milking in a parlor. Statistically significant differences were found between the type of milking bucket and milking parlor. The studies show that yeast mastitis (in subclinical and clinical form) mostly appeared in small-type dairy farms, with milking buckets and tie stalls.
The first meticillin-resistant strains of Staphylococcus aureus (MRSA) emerged in the early 1960s after the introduction β-lactamases, semi-synthetic penicillins, such as methicillin. For nearly 30 years the incidence was confined to the hospital environment (hospital-associated MRSA, HA-MRSA), which have a selective advantage over strains of S. aureus sensitive to methicillin (meticillin-susceptible Staphylococcus aureus, MSSA). CA-MRSA strains, as well as hospital strains, are capable of inducing a variety of infections; they are frequently responsible for the initial infection of the skin and soft tissues as well as necrotizing pneumonia. The factors that predispose the occurrence of MRSA are: diabetes mellitus, intravenous drug abuse, chronic hemodialysis, the colonization of the skin lesions, specific immunotherapy, neutropenia, increased IgE level, hemodialysis, surgical wound infections, stays in hospitals and nursing homes. The first reports of MRSA occurring in cattle took place in Belgium in 1972. MRSA strains have also been identified in horses, pigs, dogs, poultry and turtles. The common coexistence of VRE and MRSA can lead to the formation of MDR (multidrug resistant) strains of S. aureus. The emergence of multidrug-resistant strains is a major problem for MRSA eradication, and therefore it is necessary to discover antibacterial substances reducing the activity of the pathogens.
The aim of this study was to compare the activity of hydrolytic enzymes in yeast-like fungi isolated from cows with clinical and subclinical forms of mastitis. The study included 91 strains of fungi of the genera Candida, Trichosporon and Geotrichum isolated from cows with fungal inflammations of the udder. Fungal colonies were identified by the API 20 C AUX test (bioMerieux) and the APIWEB computer program (bioMerieux). The yeast enzyme profile was determined by the API ZYM test (bioMerieux, Poland). API ZYM is a semiquantitative micromethod capable of quickly identifying 19 enzymatic reactions. Leucine arylamidase showed the highest enzyme activity. High enzymatic activity was also shown by acid phosphatase XI, and the average activity was shown by esterase, esterase lipase, and α-glucosidase. Minimal activity (or none) was shown by α-fukosidase, α-mannosidase, β-glucuronidase, both galactosidases (α and β), α-chymotrypsin and trypsin. Our study demonstrated statistically significant differences in esterase activity between the Candida and the Trichosporon and between the Candida and the Geotrichum genera, in lipase esterase activity between the Trichosporon and fungi of the Candida and the Geotrichum genera, in â-galactosidase activity between the Candida and fungi of the Trichosporon and the Geotrichum genera, and in valine arylamidase activity between the Geotrichum and the Candida types. Yeast-like fungi produce a large number of enzymes that affect the course of a fungal infection. Enzyme activity can therefore disrupt the fungus-udder balance in favor of the fungus.
There was determined the antibiotic sensitivity of 1777 bacterial strains isolated from subclinical and clinical mastitis in cows from 20 farms. The assessment was performed by the paper disc method. It was found that Str. agalactiae was most sensitive to ampicillin (93.7%), erythromycin (88.8%), and penicillin (86.7%), Str. dysgalactiae — to chloramphenicol (96.5%), erythromycin (91.8%) and penicillin (84.6%), Str. uberis to chloramphenicol (95.6%), erythromycin (91.8%) and penicillin (88.5%), Staph. aureus to neomycin (87.0%), chloramphenicol (85.8%) and erythromycin (84.7%), Staph. epidermidis to chloramphenicol (98.1%), neomycin (87.6%) and streptomycin (77.7%), Micrococcus spp to neomycin (91.2%) and streptomycin (77.5%), E. coli to streptomycin (78.9%) and neomycin (75.0%). However, 10.1% of the strains under study appeared to be resistant to Nafpenzal.
The aim of the study was to determine IgG (total) concentrations in milk from cows with different forms of mastitis. In 51 samples of milk from black-white breed cows (15 from healthy udders, 12 from subclinical aseptic mastitis, 14 from subclinical septic mastitis and 10 from clinical mastitis) the level of IgG were measured by single radial immunodiffusion. The concentrations of IgG in mammary lacteal secretions from quarters with clinical mastitis (1477,8 mg/l) were significantly higher (p≤0,05) than in the control milk (604,6 mg/l) and in milk from subclinical aseptic (776,9 mg/l) and septic (850,1 mg/l) mastitis. There were no significant differences in concentrations of IgG in milk from aseptic and septic subclinical mastitis.
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