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Detection and identification of six Cryptospordium species in livestock in Slovakia by amplification of SSU and GP60 genes with the use of PCR analysis

100%

Danisova O., Valencakova A., Petrincova A.

Annals of Agricultural and Environmental Medicine

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2016

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tom 23

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nr 2

2

Application of specific primers in the diagnosis of Encephalitozoon spp.

100%

Valencakova A., Balent P., Novotny F., Cislakova L.

Annals of Agricultural and Environmental Medicine

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2005

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tom 12

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nr 2

321-323

In our experiment, 3 species-specific primer pairs cultivated in cell lines were used: Encephalitozoon cuniculi-specific primer pairs (ECUNF and ECUNR), Encephalitozoon hellem-specific primer pairs (EHELF and EHELR), and Encephalitozoon intestinalis-specific primer pairs (SINTF and SINTR). The PCR products were estimated to be 550 bp in E. cuniculi, 547 bp in E. hellem and 545 bp in E. intestinalis respectively, which can prove the precision and reliability of this method in the species identification of the genus Encephalitozoon. All 3 primer pairs were species-specific and none of them amplified gene sequences from other Encephalitozoon spp.

3

First detection and genotyping of human-associated Microsporidia in wild waterfowl of Slovakia

100%

Malcekova B., Valencakova A., Molnar L., Kocisova A.

Acta Parasitologica

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2013

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tom 58

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nr 1

A total of 47 avian faecal samples of wild waterfowl (great cormorant — Phalacrocorax carbo, great crested grebe — Podiceps cristatus, white stork — Ciconia ciconia) trapped in the eastern Slovakia were screened for the presence of human pathogenic microsporidia by microscopy and real-time SYBR Green PCR method using species primers and sequenced. Microscopic analysis showed presence in 32 samples (29 cormorants, 3 dippers). Microsporidial DNA (Encephalitozoon cuniculi genotype I) was identified in 19 faeces samples (40.4%) namely cormorants in 17 out of 40, one dipper of 5 and a stork out of 2. The present work describes three new host species of the bird population in microsporidium Encephalitozoon cuniculi genotype I which confirms the theory of low specificity of this species.

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First report of Enterocytozoon bieneusi and Encephalitozoon intestinalis infection of wild mice in Slovakia

88%

Danisova O., Valencakova A., Stanko M., Luptakova L., Hasajova A.

Annals of Agricultural and Environmental Medicine

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2015

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tom 22

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nr 2

5

First report of Blastocystis spp. subtypes in ZOO animals in Slovakia, Central Europe

88%

Danisova M., Valencakova A., Kandracova P., Tomko M., Sucik M.

Annals of Agricultural and Environmental Medicine

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2022

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tom 29

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nr 1

6

Encephalitozoon cuniculi infection in rabbits and laboratory mice in Eastern Slovakia

88%

Balent P., Halanova M., Sedlakova T., Valencakova A., Cislakova L.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 2

7

Immunosuppressive effect of Encephalitozoon cuniculi

88%

Valencakova A., Revajova V., Balent P., Lesnik F., Levkut M.

Bulletin of the Veterinary Institute in Puławy

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2003

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tom 47

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nr 1

8

Serological screening of selected microsporidia in HPV-positive women

76%

Adam J., Valencakova A., Halanova M., Danisova O., Gorbarova K., Cislakova L.

Acta Parasitologica

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2015

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tom 60

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nr 1

9

Detection of CHLAMYDIA PSITTACI in feral pigeons (COLUMBA LIVIA DOMESTICA) in Slovakia and their characterisation

76%

Cechova L., Halanova M., Kalinova Z., Cislakova L., Halan M., Valencakova A.

Annals of Agricultural and Environmental Medicine

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2016

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tom 23

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nr 1

10

Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health

76%

Halanova M., Valencakova A., Kvac M., Sak B., Kvetonova D., Balent P., Cislakova L.

Annals of Agricultural and Environmental Medicine

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2013

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tom 20

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nr 4

11

Serological screening of occurrence of antibodies to Encephalitozoon cuniculi in humans and animals in Eastern Slovakia

76%

Halanova M., Cislakova L., Valencakova A., Balent P., Adam J., Travnicek M.

Annals of Agricultural and Environmental Medicine

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2003

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tom 10

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nr 1

12

Chlamydiosis in farmed chickens in Slovakia and zoonotic risk for humans

64%

Cechova L., Halanova M., Babinska I., Danisova O., Bartkovsky M., Marcincak S., Marcincakova D., Valencakova A., Cislakova L.

Annals of Agricultural and Environmental Medicine

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2018

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tom 25

|

nr 2

Ograniczanie wyników

Detection and identification of six Cryptospordium species in livestock in Slovakia by amplification of SSU and GP60 genes with the use of PCR analysis

Application of specific primers in the diagnosis of Encephalitozoon spp.

First detection and genotyping of human-associated Microsporidia in wild waterfowl of Slovakia

First report of Enterocytozoon bieneusi and Encephalitozoon intestinalis infection of wild mice in Slovakia

First report of Blastocystis spp. subtypes in ZOO animals in Slovakia, Central Europe

Encephalitozoon cuniculi infection in rabbits and laboratory mice in Eastern Slovakia

Immunosuppressive effect of Encephalitozoon cuniculi

Serological screening of selected microsporidia in HPV-positive women

Detection of CHLAMYDIA PSITTACI in feral pigeons (COLUMBA LIVIA DOMESTICA) in Slovakia and their characterisation

Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health

Serological screening of occurrence of antibodies to Encephalitozoon cuniculi in humans and animals in Eastern Slovakia

Chlamydiosis in farmed chickens in Slovakia and zoonotic risk for humans