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1

1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5

100%
Dabrowski S., Pietrewicz-Kubicz E., Zablotna E., Dlugolecka A.
Acta Biochimica Polonica
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2012
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tom 59
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nr 3
 1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
2

Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol

100%
Dabrowski S., Zablotna E., Pietrewicz-Kubicz D., Dlugolecka A.
Acta Biochimica Polonica
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2012
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tom 59
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nr 3
 Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
3

Właściwości prozdrowotne imbiru

100%
Glibowski P., Dlugolecka A., Grden A., Toczek K.
Bromatologia i Chemia Toksykologiczna
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2017
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tom 50
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nr 2
4

Single-stranded DNA-binding proteins [SSBs] - sources and applications in molecular biology

100%
Kur J., Olszewski M., Dlugolecka A., Filipkowski P.
Acta Biochimica Polonica
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2005
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tom 52
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nr 3
Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination, and repair in bacteria, archaea and eukarya. The SSBs share a common core ssDNA-binding domain with a conserved OB (oligonucleotide/oligosaccharide binding) fold. This ssDNA-binding domain was presumably present in the common ancestor to all three major branches of life. In recent years, there has been an increasing interest in SSBs because they are useful for molecular biology methods and for analytical purposes. In this review, we concentrate on recent advances in the discovery of new sources of SSBs as well as certain aspects of their applications in analytical sciences.
5

Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library

76%
Cieslinski H., Bialkowska A., Tkaczuk K., Dlugolecka A., Kur J., Turkiewicz M.
Polish Journal of Microbiology
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2009
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tom 58
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nr 3
199-204
In this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. Screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip 1) out of 11000 E. coli clones. This clone harbored a plasmid, pLip 1, which has an insert of 4722 bp that was completely sequenced from both directions. Further analysis of the insert showed three open reading frames (ORFs). ORF2 encoded a protein (Lip 1) of 469 amino acids with 93% identity to the uncultured Pseudomonas sp. lipase LipJ03. Amino acid sequence comparison and phylogenetic analysis indicated that Lip 1 lipase was closely related to family I subfamily 3. Furthermore, we present a three-dimensional model of lipase Lip 1 which was generated based on the two known structures of mesophilic lipases from Pseudomonas sp. MIS 38 (PML lipase, PDB; 2Z8X) and Seiratia marcescens (SML lipase, PDB: 2QUB). Finally, we report the results of comparisons between lipase Lip 1 and mesophilic lipases and point out similarities and differences in the catalytic site and in other parts of the analyzed structures.
6

Physicochemical and biological characterization of soils from the vicinity of the Arctowski Polish Antarctic Station

76%
Bialkowska A. M., Grzelczyk A., Dlugolecka A., Cieslinski H., Kalinowska H., Kur J., Turkiewicz M.
Biotechnology and Food Science
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2012
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tom 76
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nr 1
7

Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions

76%
Dlugolecka A., Cieslinski H., Bruzdziak P., Gottfried K., Turkiewicz M., Kur J.
Polish Journal of Microbiology
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2009
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tom 58
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nr 3
211-218
In this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties. However, the differences concerning thermal activity were observed. The optimal temperature for recombinant esterase in the presence of urea (1 M) was about 15°C lower in comparison with enzyme isolated from the native source. Furthermore, the EstA was found to be more thermolabile in denaturant conditions. The differences were presumably caused by slightly changed protein structure in the presence of urea. The preservation of activity of EstA dissolved in buffer containing 8M urea suggests that the protein structure is retained and it does not undergo dramatic changes due to high urea concentration. This thesis was confirmed with FT-IR data.
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