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Samples of hair collected in 2004-2007 from 416 persons living in Central Pomerania were analyzed. The subjects donating hair represented a vast spectrum of age, from a tenmonth- old child to a 75-year-old person. The subjects were selected randomly. Lead and cadmium were determined by atomic absorption spectrophotometry using an ASA-3 spectrometer. The average content of the metals in the hair samples was 3.20 μg g–1 (Pb) and 0.284 μg g–1 (Cd). The highest concentration of lead in human hair (about 3.88 μg g–1) was determined for the age group 61-75 years, and that of cadmium (0.406 μg g–1) – for the age group 26-50 years. The lowest concentrations of these metals in human hair (2.07 and 0.152 μg g–1, respectively) were determined for the age group of 0-15 years. Most hair samples (50%) contained 2.01-4.00 μg g–1 Pb, while 45% of the samples contained 0.001- -0.300 μg g–1 of cadmium. Studying the dependence of the content of lead and cadmium in hair on the gender of subjects, it was discovered that in all age groups males had more lead and cadmium (3.79 and 0.334 μg g–1, respectively) than females (2.63 μg g–1 and 0.236 μg g–1). This study has also demonstrated that the environment affects the content of the analyzed metals in hair. The average value of lead and cadmium concentrations for people living in the country were 2.39 μg g–1 for Pb and 0.214 μg g–1 for Cd, while for the people living in towns and cities, the respective values were 4.17 and 0.361 μg g–1. The present study has demonstrated how nutrition affects lead and cadmium content in human hair. Among the subjects, 17% had been on some kind of a diet, predominatly easily digestible and light foods. The lowest content of these metals (on average, 2.08 μg g–1 Pb and 0.141 μg g–1 Cd) was found in hair of people on a diet, while the hiest levels (3.54 μg g–1 Pb and 0.315 μg g–1 Cd) were determined in people who did not limit consumption of meat and dairy products. Among the analyzed population, 241 persons suffered from chronic disease. The average content of lead and cadmium in hair of healthy subjects was 3.05 μg g–1 Pb and 0.257 μg g–1 Cd, but in patients suffering from arthrosclerosis, allergy and hyperplasia prostate the levels of lead and cadmium in hair reached the upper values of the se limits. Hair of the patients who suffered from cardiovascular disease showed deificiency of these metals (on average, 1.73 μg g–1 Pb and 0.182 μg g–1 Cd).
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Copper in human hair of Middle Pomeranian population

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The scalp hair has been suggested as a potentially valuable tissue, where short-term variation are averged out. Besides, hair has been demonstrated to be a major vehicle for the excretion of toxic metals, which concentration may be to 10 times higher than the amount found in blood or urine. Undoubtedly, hair analysis is a non-invasive method of diagnosis. The content of metals in hair depends on many factors. Hair samples (N = 416) were collected between the years 2003 and 2004 from Middle Pomerania (north Poland) population. Cu was determined by the electro-thermal graphite furnance AAS method. Hair from Middle Pomerania people contented in average 12.7 μg g-1 (range 5.2-32.0 μg g-1). The level of copper in hair was the function of age, sex, obesity, nutrition and disease, but was not the function of stature and weight. The obtained results were confirmed in comparison with the findings of other authors. Hair analysis might soon become a powerful diagnostic tool in monitoring of environmental exposure and studying the nutritional status of individuals.
The aim of this work was the assessment of the age, sex, way of nutrition, environment and health state influence on zinc content in hair of population living in the Middle Pomerania. Within years of 2005-2007 hair coming from 416 persons from a ten months old child to a 75 years old person was analyzed. All people were divided into four age groups: children (0-15 years), youth (16-25), adults (26-50) and the elderly (51-75). 3-centimeters long hair (counting from the skin) were put to chemical analyze. Then dried samples were weighed and mineralized with mixture of nitric and tetraoxochloric acides (mixed in proportion of 5:1). Zink determination was done by method of atomic absorption spectrophotometry using spectrometer ASA-3. Average zinc content in hair of researched population amounted to 167±58 7g g-1. Least of all zinc was ascertained in children hair (av. 125 7g g-1), and most of all in youth (194 7g g-1). In all age groups females had more of this metal (av. 180 7g g-1) than males (156 7g g-1). Average value of zinc for people living in a village was 147 7g g-1, and living in towns and cities 181 7g g-1. The least of zinc we have found at persons being on the meat- or milk-free diet (av. 157 7g g-1) and the most at persons that did not restrict in eating meat and milk products (197 7g g-1). The persons suffering from cardiovascular disease or hyperplasia prostate had adequately 221 and 195 7g g-1 Zn, considerably above the level in hair of without symptoms diseases persons (156 7g g-1). Smaller content of this element had persons suffering from atherosclerosis or hypertension (adequately 132 and 142 7g g-1).
HPV DNA was amplified by the nested-PCR method to identify E6/E7 HPV 16 from 72 deep-frozen (-70°C) specimens of LSCC tumours collected after a total laryngectomy. HPV was identified in 38.9% of the specimens. There was no statistically significant correlation between the presence of HPV and epidemiological, clinical, and pathological features, and recurrence. There was no statistically significant correlation between the presence of HPV and survival. HPV infection is not likely to influence survival rates as an independent prognostic factor in patients with laryngeal cancer. The presence of HPV infection in 38.9% of the cases suggest a possible role in the aetiology of laryngeal cancer and support the role of high-risk types of HPV 16 in LSCC.
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