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1

Immunogold silver staining method for light microscopic detection of peripheral blood lymphocyte surface antigens with monoclonal antibodies

100%
Levkutova M., Levkut M., Bajova V.
Folia Histochemica et Cytobiologica
|
1992
|
tom 30
|
nr 3
103-105
2

Larval toxocarosis in mice: immunoreactivity after multiple high dose infections

100%
Aldawek A. M., Levkut M., Revajova V., Dvoroznakova E.
Acta Parasitologica
|
2002
|
tom 47
|
nr 3
Immunological responses of mice to threefold infection with infective Toxocara canis were studied up to 42 days. Mice of the experimental group were infected orally with 1000 infective eggs on days 0, 12, and 20 of the experiment (a total of 3000 eggs/mouse). The proliferative activity of spleen lymphocytes, production of specific antibodies in the serum, leukocyte counts, and T cells subpopulations in the blood and spleens were examined. The spleen T cells were significantly inhibited to 21 day post infection (dpi) while B cells were stimulated. An increased level of specific antibodies persisted almost the whole experiment. Leukocytosis with a dominant lymphocytosis was evident. In the peripheral blood the number of both CD8⁺ and CD4⁺ cells was significantly increased. Contradictory, a significant increase of the spleen CD8⁺ cells with a significant decrease of CD4⁺ cells was observed. In conclusion, the multiple high dose infection resulted in variable changes in the immune effector mechanisms. Some components were stimulated while others were inhibited which may suggest that immunosuppression is not the only major outcome of larval toxocarosis.
3

Histiocytic Ulcerative Gastroenterocolitis and Dermatoneoplasm in Boxer Dog - Clinical Case Report

100%
Kozak M., Levkut M., Revajova V., Magic D.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 12
4

Monoclonal antibodies recognizing bovine leukocyte antigens used in immuno-scanning electron microscopy

100%
Levkut M., Ponti W., Levkutova M., Quirici N.
Folia Histochemica et Cytobiologica
|
1995
|
tom 33
|
nr 3
5

Mast cells and eosinophils in rat mammary gland tumours induced by N-Nitroso-N-methylurea

88%
Kissova V., Sevcikova Z., Revajova V., Herich R., Levkut M.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 3
The aim of the study was to evaluate the distribution and number of mast cells and eosinophils in rat mammary gland tumours induced by N-Nitroso-N-methylurea. The highest density of mast cells was found in cystic papillary adenocarcinomas of grade II. Eosinophils were detected only in the cystic papillary adenocarcinoma of grades I and II, in non-invasive cribriform adenocarcinoma and comedo-type carcinoma. Mast cell populations were observed perivascularly in the tumour stroma, in the host tumour interface, as well as in necrotic areas of neoplasms. Mast cells were observed to be intact according to their morphological changes, collectively referred to as degranulation. The obtained results indicate that mast cells and eosinophils play an important role in tumour micro-environment formation. The increased density of these cells in experimentally-induced rat mammary gland tumours suggests a poor prognosis in these cancers. Our results also confirmed that rat mammary gland tumours are good models for the study of breast cancers.
6

Surface analysis of IgM-expressing bovine lymphocytes in BLV-infected cattle by immuno-scanning electron microscopy

88%
Levkut M., Ponti W., Levkutova M., Turin L., Poli G.
Folia Histochemica et Cytobiologica
|
1996
|
tom 34
|
nr 1
7

Device for quantitative collection of chicken excrements in balance studies

88%
Gresakova L., Boldizarova K., Kuricova S., Levkut M., Leng L.
Journal of Animal and Feed Sciences
|
2003
|
tom 12
|
nr 2
8

Immunosuppressive effect of Encephalitozoon cuniculi

88%
Valencakova A., Revajova V., Balent P., Lesnik F., Levkut M.
Bulletin of the Veterinary Institute in Puławy
|
2003
|
tom 47
|
nr 1
9

Changes in integrin-positive cells and T cell subpopulations in the peripheral blood and intestine of calves fed soya protein

76%
Brna M., Revajova V., Zitnan R., Levkut,Jr. M., Baran M., Levkut M.
Journal of Animal and Feed Sciences
|
2010
|
tom 19
|
nr 3
In order to examine the relation of known intestinal lesions to changes in T-cell phenotypes and integrin expression, 16 male 10-day-old Holstein calves were divided into two groups. For 28 days of the experiment, eight males were fed NutriMilk in which 50% of the crude protein was soya protein, and eight control animals, with NutriMilk containing only milk casein. The animals fed soya protein showed shorter jejunal villi with a corrugated surface and deeper crypts compared with the control calves. A higher density of CD8+ cells in the intestinal mucosa and a decrease of these cells in peripheral blood were found in calves fed soya protein. The number of CD11b-positive cells was decreased in the peripheral blood of calves fed soya protein. Lower expression of integrin could be related to the appearance of non-mature polymorphonuclear cells. It is not clear if the decrease in CD11b expression on blood cells could also be influenced by milk replacer, i.e. soya protein.
10

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

76%
Sevcikova Z., Blanar J., Laukova A., Revajova V., Strompfova V., Levkut M.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 3
11

Polymerase chain reaction detection of caecal bacteria in case of preventive application of Enterococcus faecium EK13 against Salmonella enterica subsp. Enteritidis in chickens

76%
Herich R., Laukova A., Strompfova V., Revajova V., Levkut M., Pistl J.
Journal of Animal and Feed Sciences
|
2005
|
tom 14
|
nr 1
12
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Prevalence of antibodies to Encephalitozoon cuniculi in horses in Israel

76%
Levkutova M., Hipikova V., Faitelzon S., Benath G., Paulik S., Levkut M.
Annals of Agricultural and Environmental Medicine
|
2004
|
tom 11
|
nr 2
Infection with the intracellular microsporidium Encephalitozoon cuniculi can cause a serious disease - encephalitozoonosis in various animals and people. Several species of mammals, including the horse, were seem to be potential sources of encephalitozoonosis for animal as well as human hosts. The disease diagnosis is based on clinical signs, pathological findings, and the detection of E. cuniculi or circulating antibodies directed against the parasite. This study investigates the seroconversion to E. cuniculi in horses admitted to the Veterinary Teaching Hospital of the Hebrew University of Jerusalem and 3 different private horse-riding farms across Israel. Antibodies to E. cuniculi were determined using the IFA test in the sera from 102 horses. Of 72 asymptomatic horses, 60% were seropositive and 19% of the positive samples showed a titter of 1:512. Of 30 horses with various clinical signs, 80% were seropositive and 68% of the positive samples showed a titer of 1:512. High titers were associated with colic and neurological signs. This could prove to be interesting if the high percentages of prevalence of antibodies level in horses are an indication of health risk in humans.
13

Prevalence of anti-microsporidial antibodies in randomly examined dogs in Eastern Slovakia

76%
Herich R., Levkutova M., Kokincakova T., Reiterova K., Hipikova V., Levkut M.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 05
Sixty-eight dogs were examined for the presence of Encephalitozoon spp. antibodies. Twenty-one dogs (30.9%) were healthy without any clinical signs of diseases. Forty-seven animals (69.1%) developed clinical symptoms of diseases such as chronic otitis externa, conjunctivitis, upper respiratory tract inflammation, status epilepticus, pyodermatitis, skin hypersensitivity, demodicosis, flea allergy. Different detection methods of encephalitozoonosis including IFAT (Indirect Immunofluorescence Antibody Assay), in vitro cultivation, SDS PAGE electrophoresis, Western blot and PCR were applied. There were 33 (48.5%) positive reacting sera to E. cuniculi II (mouse type) antigen using IFAT, including 9 positive samples obtained from clinically healthy dogs. Sixteen samples with the antibody titer equal to 1 : 256 were then tested by Western Blot. Most of the samples reacted with E. cuniculi II and III antigens. The presence of E. intestinalis antibodies was lower and just a few samples reacted with E. hellem antigen. The electrophoretic analysis of the encephalitozoon strains used as antigens confirmed that they differ primarily in the molecular size. The strain of type II (mouse) expressed a double strip at 54 and 58 kDa level. The strain of type III (dog) expressed a wide strip at 59 kDa. E. cuniculi types II and III are more related in protein structure in comparison to the other analyzed strains. When PMP1/PMP2 primers were used in PCR, the size of the amplified product was 268 bp for E. cuniculi and 270 bp for E. intestinalis. A species-specific primer pair for E. cuniculi ECUNF/ECUNR gave a 549 bp fragment and V1/SI-500 primers specific for E. intestinalis gave a 370 bp fragment.
14

Antioxidant enzyme activities in liver tissue of chickens fed diets supplemented with various forms and amounts of selenium

64%
Holovska K., Holovska K., Boldizarova K., Cekonova S., Lenartova V., Levkut M., Javorsky P., Leng L.
Journal of Animal and Feed Sciences
|
2003
|
tom 12
|
nr 1
15

Eimeria colchici and immunocompetent cells in specific and non-specific hosts

64%
Goldova M., Revajova V., Pistl J., Letkova V., Levkut M., Wagshal E., Csizsmarova G., Looszova A.
Acta Parasitologica
|
2001
|
tom 46
|
nr 1
16

Influence of Encephalitozoon cuniculi experimental infection on antirabies immunity in a mouse model

52%
Franka R., Svrcek S., Suli J., Benisek Z., Madar M., Hipikova V., Levkutova M., Zavadova J., Ondrejkova A., Ondrejka R., Levkut M., Revajova V.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 3
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