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Prolific differentiation of shoot buds of Leucaena leucocephala was induced from the different plant parts viz. cotyledon, hypocotyl and leaf. Adventitious shoot bud formation was recorded with prudent application of N6-2- (isopentenyl) adenosine and 15 % (v/v) coconut water. Coconut water alone was unable to produce any beneficial effect with regard to the shoot bud proliferation but the response was augmented with the increase in concentration of N6-2- (isopentenyl) adenosine. However supra-optimal level of N6-2- (isopentenyl) adenosine was inhibitory. Best response was recorded from the cotyledon explant at 2 mg·dm-3 N6-2- (isopentenyl) adenosine compared to the other two explants. Comparative assessment was undertaken following the same experimental protocol in liquid shake culture. The regenerated shoot buds were subcultured in plant growth regulator-free medium where leafy shoot emergence was recorded. Optimum regeneration of roots was observed in these shoots in presence of 1 mg dm-3 α-naphthalene acetic acid. Plantlets were finally hardened following standard procedures before transplantation to the field. In another experimental set up, the de-embryonated cotytedons regenerated shoot buds via callus formation. The regenerated shoots and plantlets obtained through callus mediated organogenesis could be used for rapid multiplication and also for the genetic improvement of individual clones of Leucaena leucocephala.
The present paper dealt with the annual dynamics of the absorption coefficient of chromophoric dissolved organic matter at 440 nm {aCDOM(440)} during February 2015 to January 2016 in the continental shelf of northern Bay of Bengal (nBoB) for the first time. Sea surface salinity (SSS), chlorophyll-a (Chl-a), total suspended matter (TSM) were also analyzed. It was hypothesized that CDOM should exhibit significant spatial and temporal variability in this region. aCDOM(440) and spectral slope ranged between 0.1002 m1—0.6631 m1 and 0.0071 nm1— 0.0229 nm1 respectively during the entire study period. Higher values of aCDOM(440) were observed in the near shore stations and gradually decreased towards the offshore. Significant seasonal variability of aCDOM(440) was observed between the monsoon and non-monsoon seasons ( p < 0.05). Thus the framed hypothesis was successfully accepted by means of the present study. The CDOM was mainly found to be of allochthonous character in this region. aCDOM(440) portrayed a significant negative linear relationship with SSS (R2 = 0.80; p < 0.05) implying conservative mixing of marine and terrestrial end members. However, examining the spatial variability of the relationship, it was observed that this relationship was significant only in the nearshore stations. While examining the seasonal variability of this relationship, it was found to be most significant during the monsoon (R2 = 0.81; p < 0.05). Thus it was inferred that whenever the SSS gradient was higher, the relationship between aCDOM(440) and SSS was found to be most significant.
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