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Somaclonal variation in winter wheat [Triticum aestivum L.]: frequency, occurrence and inheritance

100%

Cheng X. Y., Gao M. W., Liang Z. Q., Liu G. Z.

Journal of Applied Genetics

1998

tom 39

nr 1

Plants were regenerated from immature embryo cultures of 35 winter wheat genotypes. General responses of regenerated plants were investigated and a total of 7142 R₂ spike lines from 1593 R₁ plants were assessed in the field for somaclonal variants in 1985/86, 1986/87 and 1987/88. Selected variants were studied for their possible genetic inheritance. From regenerated plantlets, 81% survived and 63% produced fertile plants. Forms with reduced plant height, length of spike and other morphological abnormalities were found in this progeny. Populations of R₁ plants were highly variable due mainly to the physiological disturbances resulting from the in vitro process. Overall somaclonal variation frequencies were 14.2% per plant basis and 5.3% per R₂ spike basis. The variants were similar in the three different R₂ generations with predominant variants being negative in plant height, maturity, awns, spike type and plant type. Both uniform R₂ variant families and spike lines were found in addition to the segregating variants which constituted the majority. On average, in a variant family or line, 18% and 14% of their component lines and plants were variants, respectively. Inheritability was demonstrated for the uniform variant families and spike lines as well as segregated variants. Of those 134 selections, about 70% were classified as inheritable. Both recessive and dominant gene mutations at one, two or three loci were evident in some variants as suggested by the segregating data.

Primary culture of cat intestinal epithelial cells in vitro and the cDNA library construction

64%

Zhao G. H., Liu Y., Cheng Y. T., Zhao Q. S., Qiu X., Xu C., Xiao T., Zhu S., Liu G. Z., Yin K.

Acta Parasitologica

2018

tom 63

nr 2

Ograniczanie wyników

1 Acta Parasitologica

1 Journal of Applied Genetics

2 Liu G. Z.

1 Cheng X. Y.

1 Cheng Y. T.

1 Gao M. W.

1 Liang Z. Q.

1 Liu Y.

1 Qiu X.

1 Xiao T.

1 Xu C.

1 Yin K.

1 Zhao G. H.

1 Zhao Q. S.

1 Zhu S.

1 2018

1 1998

Wyniki wyszukiwania

Somaclonal variation in winter wheat [Triticum aestivum L.]: frequency, occurrence and inheritance

Primary culture of cat intestinal epithelial cells in vitro and the cDNA library construction