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Role of nitric oxide in the control of the gastric motility within the nucleus ambiguus of rats

100%

Sun H.-Z., Zhao S.-Z., Ai H.-B.

Journal of Physiology and Pharmacology

2012

tom 63

nr 6

Highly efficient Agrobacterium-based transformation sysyem for callus cells of the C3 halophyte Suaeda salsa

100%

Zhao S.-Z., Ruan Y., Sun H.-Z., Wang B.-S.

Acta Physiologiae Plantarum

2008

tom 30

nr 5

Genetic manipulation technologies have been limited in the halophyte Suaeda salsa L. due to the lack of an efficient transformation system. Here, we examined factors affecting transformation and developed an efficient transformation system at the cell level using S. salsa hypocotyl as starting material. S. salsa hypocotyl explants from 10-day-old seedlings were precultured for 2 days on a hygromycin (hyg)-free callus induction medium (CIM) and then inoculated with Agrobacterium tumefaciens suspension at a concentration of 0.5 at OD600 for 5–10 min. After cocultivation with A. tumefaciens for 4 days in the dark, followed by selection on carbenicillin (carb) for 3 days, explants were placed on CIM containing 10 mg l⁻¹ hyg and 500 mg l⁻¹-carb with three to four consecutive subcultures for up to 45 days. β-Glucuronidase assays showed an average transformation frequency of 62.89%. Gene integration was confirmed by polymerase chain reaction analysis and Northern blot analysis. To our knowledge, this is the first study to show Agrobacterium-mediated transformation in the C3 halophyte S. salsa.

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Hydrogen sulfide modulates gastric acid secretion in rats via involvement of substance P and nuclear factor-kappaB signaling

51%

Sun H.-Z., Gong X.-Y., Wu L., Wang X.-X., Nie Y.-N., Shang R., Wang H., Li Y.-C., Sun Q.-F., Gao P.-F., Bi J.-X.

Journal of Physiology and Pharmacology

2018

tom 69

nr 3

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Acta Physiologiae Plantarum

3 Sun H.-Z.

2 Zhao S.-Z.

1 Ai H.-B.

1 Bi J.-X.

1 Gao P.-F.

1 Gong X.-Y.

1 Li Y.-C.

1 Nie Y.-N.

1 Ruan Y.

1 Shang R.

1 Sun Q.-F.

1 Wang B.-S.

1 Wang H.

1 Wang X.-X.

1 Wu L.

1 2018

1 2012

1 2008

Wyniki wyszukiwania

Role of nitric oxide in the control of the gastric motility within the nucleus ambiguus of rats

Highly efficient Agrobacterium-based transformation sysyem for callus cells of the C3 halophyte Suaeda salsa

Hydrogen sulfide modulates gastric acid secretion in rats via involvement of substance P and nuclear factor-kappaB signaling