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Effective population control of Japanese wild boar (Sus scrofa leucomystax) requires reliable information about population dynamics. Fertility rate is the fundamental component of reproduction to evaluate population dynamics. However, little is known regarding the fertility rate of Japanese wild boar. The traditional hunting practices make it difficult to obtain pregnant females and calculate the fertility rate by checking fetuses as is performed in other countries. Therefore, we focused on the corpora albicans (CA) as the CA remains in the ovaries of postpartum females after pregnancy. This study aimed to evaluate the utility of CA and estimate the fertility rate of Japanese wild boars using CA. Histological analysis of ovaries enabled us to discriminate type 1 CA, which remains for 1 year after breeding. Type 1 CA is a superior indicator compared with lactation in the non-pregnancy season because it allows verification of postpartum females over a long period. The fertility rate was calculated by the combination of pregnant and postpartum females using fetuses and type 1 CA from April to November. The fertility rate of the females captured after the second pregnancy season was 90.3 % during the pregnancy period and 100 % during the non-pregnancy period. The high fertility rate of adult females suggests that intensive adult female harvesting is needed. Our new method to determine fertility rates contributes to developing a monitoring system to adequately control Japanese wild boar population.
We analyzed seasonal and sexual fluctuations in kidney mass (KM) and kidney fat mass (KFM) as indices of condition in Hokkaido sika deer Cervus nippon yesoensis Heude, 1884. For 76 male and 132 female sika deer, seasonal fluctuations in KM and KFM were given by fitted sine wave growth curves. Although the kidney fat index (KFI) is used frequently to evaluate animal condition, we reject it because it is based on the assumption that kidney mass is proportional to body mass in all seasons. Our data did not support this assumption. KFM is a better indicator of Hokkaido sika deer condition than KFI. Although sex-based differences in cervid KFM are said to reflect differences in reproductive cycles, the seasonal similarities in sika deer KFM levels may represent adaptations to the long severe Hokkaido winter. Because in our study deer populations were at low densities and had high pregnancy rates, our sine wave growth models can be regarded as reference for fat level fluctuations in Hokkaido sika deer.
Two nematode species of the genus Philometra Costa, 1845 (only females), P. ocularis sp. nov. and P. managatuwo Yamagutl, 1941 were recorded from the ocular cavity and the gonad, respectively, of the serranid fish (grouper) Epinephelus septemfasciatus (Thunberg) from the coast of the Tsushima Island, Nagasaki Prefecture, Japan. The new species is mainly characterized by the presence of four conspicuously large, crescent-shaped, fleshy cephalic papillae of the external circle and two small, subterminal papilla-like projections on the caudal end. Philometra cephalus is transferred to the genus Philometroides Yamaguti, 1935 as P. cephalus (Ramachandran, 1975) comb. nov. The finding of P. managatuwo represents the second record of this species and a new host record.
Hypothetical chloroplast reading frame 5 (ycf5) is encoded by the chloroplast genome and potentially specifies a protein of 313 amino acids in tobacco. A northern blot analysis showed that ycf5 transcripts accumulated at substantial levels in the chloroplasts, but not in the proplastids of nonphotosynthetic cultured cells. In an attempt to determine the function of ycf5, we constructed mutant alleles for the targeted deletion of ycf5. The mutant allele was introduced into the tobacco chloroplast genome by biolistic chloroplast transformation to replace the corresponding wild-typeycf5 alleles by homologous recombination. Homoplasmic ycf5-knockout tobacco plants displayed a pale-green phenotype. An analysis of the transient increase in chlorophyll fluorescence suggested that the electron flow around photosystem II (PSII) was completely impaired in the ycf5-deficient transformants. These findings indicate that ycf5 is indeed a functional gene, and that its gene product is involved in the generation of functional PSII units. This is the first report of the isolation and characterization of ycf5-knockout mutants in higher plants.
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