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The study addressed different biological aspects of Juniperus oxycedrus subsp. macrocarpa, including female cone production, biometric analysis of mature cones and seeds, and their viability. The results indicate that the proportion of aborted female cones was very high. In the four populations studied, cone diameter and weight and seed length and weight were closely related. The distribution of numbers of seeds with and without embryos in mature cones was examined, as well as the frequency of numbers of seeds with embryos. The general trend in all populations was three seeds per mature cone, of which only one or two showed embryos. Tetrazolium tests showed the percentage of viable seeds to be lower.
Juniperus oxycedrus subsp. macrocarpa is an endangered species in southwest Spain, with seed dormancy as found in other species of the same genus. This study employed different experiments to determine a method to improve the seedling emergence in this species. Three types of seedling emergence trials were performed: (a) untreated seeds under greenhouse conditions, (b) untreated seeds under natural conditions, and (c) treated seeds under greenhouse conditions, with different acids (sulphuric, hydrochloric and nitric) for 10 and 30 min, followed or not by cold stratification for 3 months. In all trials, seeds derived from both mature and immature cones were used to verify which one produced higher seedling emergence. Previously, seed viability was verified and a proper substrate for greenhouse sowing was selected. The best percentage of seedling emergence was obtained in the "a” and "b" trials. In trial "a", seeds derived from immature cones germinated significantly better than mature ones. Chemical scarification of seeds with or without cold stratification yielded less seedling emergence than the other trials.
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