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For the period of 4 years, in quarter-time cycle, samples had been taken from surface microlayers and subsurface water of the lakes: Gardno, Jasień and Dołgie Wielkie, by two techniques (Garret net and glass plate). In each talked over layers we have analyzed contents of calcium, general alka-linity, conductance and water reaction (pH). We have also determined the ability of surface mi-crolayer to accumulate these components and we have compared it to subsurface water ability and verified whether this ability depends on the kind of the lake.
The surface microlayer is a thin layer found at the contact zone of two environments forming an ecotone at the atmosphere–hydrosphere interface. It is the boundary layer of extreme importance for both environments. In the years 2000–2004 surface water microlayer of an eutrophic, deep lake (577 ha, max. depth 32.2 m, Lake Jasień in Northern Poland) was analyzed (in four seasons each year) in terms of their capacity to accumulate lead, cadmium and manganese. The following surface water layers were collected using the different devices (plate method, net method) and compared: a microlayer of approx. 100 μm in thickness (SM), a microlayer of approx. 240 μm in thickness (PM) and subsurface water (about 20 cm below surface) (SSW). The highest concentrations of analyzed metals were observed in the microlayer of 100 μm, characterized by enrichment factor (EF-ratio of surface to subsurface values) of 2.61 for cadmium, 2.55 for manganese and 1.57 for lead, respectively. Enrichment factors for these metals are much higher than in respective microlayers of brackish lake situated close and studied before. Seasonal changes in concentrations of analyzed elements were correlated with changes in their concentrations in subsurface water. Statistical analysis of results showed that during a year variation in Cd and Mn accumulation in analyzed layers was significant. In contrast, variation in Pb accumulation was statistically non-significant. It means that in the surface microlayer of lake water a seasonal cycle is observed for changes in heavy metal concentrations and this variability in concentrations of analyzed substances is not accidental, but definitely periodical in character. The accumulation of analyzed heavy metals in the surface microlayers of the studied deep eutrophic lake seems to be a regular phenomenon.
Samples of hair collected in 2004-2007 from 416 persons living in Central Pomerania were analyzed. The subjects donating hair represented a vast spectrum of age, from a tenmonth- old child to a 75-year-old person. The subjects were selected randomly. Lead and cadmium were determined by atomic absorption spectrophotometry using an ASA-3 spectrometer. The average content of the metals in the hair samples was 3.20 μg g–1 (Pb) and 0.284 μg g–1 (Cd). The highest concentration of lead in human hair (about 3.88 μg g–1) was determined for the age group 61-75 years, and that of cadmium (0.406 μg g–1) – for the age group 26-50 years. The lowest concentrations of these metals in human hair (2.07 and 0.152 μg g–1, respectively) were determined for the age group of 0-15 years. Most hair samples (50%) contained 2.01-4.00 μg g–1 Pb, while 45% of the samples contained 0.001- -0.300 μg g–1 of cadmium. Studying the dependence of the content of lead and cadmium in hair on the gender of subjects, it was discovered that in all age groups males had more lead and cadmium (3.79 and 0.334 μg g–1, respectively) than females (2.63 μg g–1 and 0.236 μg g–1). This study has also demonstrated that the environment affects the content of the analyzed metals in hair. The average value of lead and cadmium concentrations for people living in the country were 2.39 μg g–1 for Pb and 0.214 μg g–1 for Cd, while for the people living in towns and cities, the respective values were 4.17 and 0.361 μg g–1. The present study has demonstrated how nutrition affects lead and cadmium content in human hair. Among the subjects, 17% had been on some kind of a diet, predominatly easily digestible and light foods. The lowest content of these metals (on average, 2.08 μg g–1 Pb and 0.141 μg g–1 Cd) was found in hair of people on a diet, while the hiest levels (3.54 μg g–1 Pb and 0.315 μg g–1 Cd) were determined in people who did not limit consumption of meat and dairy products. Among the analyzed population, 241 persons suffered from chronic disease. The average content of lead and cadmium in hair of healthy subjects was 3.05 μg g–1 Pb and 0.257 μg g–1 Cd, but in patients suffering from arthrosclerosis, allergy and hyperplasia prostate the levels of lead and cadmium in hair reached the upper values of the se limits. Hair of the patients who suffered from cardiovascular disease showed deificiency of these metals (on average, 1.73 μg g–1 Pb and 0.182 μg g–1 Cd).
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Copper in human hair of Middle Pomeranian population

51%
The scalp hair has been suggested as a potentially valuable tissue, where short-term variation are averged out. Besides, hair has been demonstrated to be a major vehicle for the excretion of toxic metals, which concentration may be to 10 times higher than the amount found in blood or urine. Undoubtedly, hair analysis is a non-invasive method of diagnosis. The content of metals in hair depends on many factors. Hair samples (N = 416) were collected between the years 2003 and 2004 from Middle Pomerania (north Poland) population. Cu was determined by the electro-thermal graphite furnance AAS method. Hair from Middle Pomerania people contented in average 12.7 μg g-1 (range 5.2-32.0 μg g-1). The level of copper in hair was the function of age, sex, obesity, nutrition and disease, but was not the function of stature and weight. The obtained results were confirmed in comparison with the findings of other authors. Hair analysis might soon become a powerful diagnostic tool in monitoring of environmental exposure and studying the nutritional status of individuals.
The aim of this study was to compare ecophysiological basis for developing feral pigeons (Columba livia f. urbana) in various environments of Northern Poland. We examined heavy metals contents, lipid and protein peroxidation, antioxidant enzymes activity in individuals growing and feeding in the different polluted regions. Pigeons from urban area possessed high maintenance of cadmium in the blood, but low lead in comparison to birds from rural area. Our results suggest that increased level of heavy metals (Pb and Cd) in the blood of pigeons from different regions of Northern Poland tended to affect negatively initiate lipid peroxidation and increased oxidative modified protein content. Our results suggest that increased level of oxidative stress in birds is dependent upon environmental pollution. Statistical analysis (ANOVA and GLM) has shown that colony localization (urban or rural areas) modified antioxidative defense system, level of lipid and protein peroxidation, and blood total antioxidant activity.
In July 2000 the diurnal cycle of analyses was conducted in order to determine variation in the concentrations of nitrogen and calcium compounds as well as dissolved gases in surface microlayers of a shallow coastal barrier lake located at the Baltic Sea coast. Water for analyses was collected in a diurnal cycle for the period of 24 h from lake Dołgie Wielkie. The analyses were conducted on the surface microlayer with a thickness of approx. 100 μm, a layer with a thickness of approx. 250 6m and a layer of subsurface water from a depth of 15 cm. Based on the analyzed data, information was collected on the migration of nitrogen compounds between the analyzed layers. Changes were observed in nitrogen forms reduced to oxidized forms and vice versa.
This paper presents results of chemical and bacteriological examinations of surface and subsurface water layers of estuarine lake Gardno. The obtained data indicate that there are substantial differences in chemical compound concentrations and also bacteria number and activity between the water layers under investigation. Particularly great differences between microlayers and subsurface water refer to concentrations of organic phosphorus and nitrogen. It was found that bacteria number was greater in surface water layers than in subsurface water. Significant differences among the studied chemical and bacteriological parameters were revealed between particular sites across lake Gardno. The number of freshwater, brackish and marine bacteria in the water of lake Gardno was up to chlorides concentration.
Higher nutrients concentrations were observed into the surface microlayer then in the subsurface of estuarine, shallow lake water. The thinner the surface microlayer, which means the closer it is to the water-atmosphere contact zone, the higher are concentrations of organic and inorganic phosphorus, as well as organic and ammonia nitrogen. Mean values of enrichment coefficients were usually positive. The highest values of these coefficients were found for organic phosphorus and organic nitrogen in relation to phosphates, ammonium nitrogen and nitrate nitrogen.
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Heavy metals in some peat soils of Middle Pomerania

45%
The content of heavy metals in peat soils of north part of Słupsk Region was low. Enrichment factors for Cd, Zn and Mn indicates on their anthropogenic origin in studied peat soils, and for Cr and Ni - natural origin. However copper may be of anthropogenic or natural origin depending on location. The farther from sea peat soils are situated the higher concentration of those metals is.
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The aim of this work was the assessment of the age, sex, way of nutrition, environment and health state influence on zinc content in hair of population living in the Middle Pomerania. Within years of 2005-2007 hair coming from 416 persons from a ten months old child to a 75 years old person was analyzed. All people were divided into four age groups: children (0-15 years), youth (16-25), adults (26-50) and the elderly (51-75). 3-centimeters long hair (counting from the skin) were put to chemical analyze. Then dried samples were weighed and mineralized with mixture of nitric and tetraoxochloric acides (mixed in proportion of 5:1). Zink determination was done by method of atomic absorption spectrophotometry using spectrometer ASA-3. Average zinc content in hair of researched population amounted to 167±58 7g g-1. Least of all zinc was ascertained in children hair (av. 125 7g g-1), and most of all in youth (194 7g g-1). In all age groups females had more of this metal (av. 180 7g g-1) than males (156 7g g-1). Average value of zinc for people living in a village was 147 7g g-1, and living in towns and cities 181 7g g-1. The least of zinc we have found at persons being on the meat- or milk-free diet (av. 157 7g g-1) and the most at persons that did not restrict in eating meat and milk products (197 7g g-1). The persons suffering from cardiovascular disease or hyperplasia prostate had adequately 221 and 195 7g g-1 Zn, considerably above the level in hair of without symptoms diseases persons (156 7g g-1). Smaller content of this element had persons suffering from atherosclerosis or hypertension (adequately 132 and 142 7g g-1).
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