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Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima

100%

Huang Z., Long H., Jiang Q.-T., Wei Y.-M., Yan Z.-H., Zheng Y.-L.

Journal of Applied Genetics

2010

tom 51

nr 1

9-18

Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS genes from Ae.longissima Schweinf. & Muschl., a species of the section Sitopsis of the genus Aegilops, which is closely related to the В genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the В genome, and the remaining was related to those from A and D genomes of wheat and Ae. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats and Ae. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members of LMW-GS multi-gene famailies. These results suggested that the Sitopsis species of the genus Aegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes from Ae. longissima into wheat.

Characterization of omega-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line

100%

Jiang Q.-T., Wei Y.-M., Andre L., Lu Z.-X., Pu Z.-E., Peng Y.-Y., Zheng Y.-L.

Journal of Applied Genetics

2010

tom 51

nr 4

Sixty-two DNA sequences for the coding regions of omega-secalin (ω-secalin) genes have been characterized from rye (Secale cereale L.), hexaploid and octoploid triticale (× Triticosecale Wittmack), and wheat (Triticum aestivum L.) 1BL/1RS translocation line. Only 19 out of the 62 ω-secalin gene sequences were full-length open reading frames (ORFs), which can be expressed into functional proteins. The other 43 DNA sequences were pseudogenes, as their ORFs were interrupted by one or a few stop codons or frameshift mutations. The 19 ω-secalin genes have a typical primary structure, which is different from wheat gliadins. There was no cysteine residue in ω-secalin proteins, and the potential celiac disease (CD) toxic epitope (PQQP) was identified to appear frequently in the repetitive domains. The ω-secalin genes from various cereal species shared high homology in their gene sequences. The ω-secalin gene family has involved fewer variations after the integration of the rye R chromosome or whole genome into the wheat or triticale genome. The higher Ka/Ks ratio (i.e. non-synonymous to synonymous substitutions per site) in ω-secalin pseudogenes than in ω-secalin ORFs indicate that the pseudogenes may be subject to a reduced selection pressure. Based on the conserved sequences of ω-secalin genes, it will be possible to manipulate the expression of this gene family in rye, triticale, or wheat 1BL/1RS translocation lines, to reduce its negative effects on grain quality.

Ograniczanie wyników

2 Journal of Applied Genetics

2 Jiang Q.-T.

2 Wei Y.-M.

2 Zheng Y.-L.

1 Andre L.

1 Huang Z.

1 Long H.

1 Lu Z.-X.

1 Peng Y.-Y.

1 Pu Z.-E.

1 Yan Z.-H.

2 2010

Wyniki wyszukiwania

Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima

Characterization of omega-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line