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Complex investigations into the metabolism of T. spiralis infected muscles showed, among others, an increase in the metabolism of high energy phosphorus compounds (Garbuliński et al., 1965). The results obtained with P32 held true for the whole muscular tissue containing both infected and non infected fibres. To conform these finding and reach more exact data about the localization of the occuring processes, autoradiographic method was recurred to. P32 was injected to normal and T. spiralis infected mice 11, 19 days or 8 weeks later; the animals were sacrificed 15 and 30 minutes, 1, 3, 6, and 24 hours since isotope injection, mean values of emulsion darkenings over the morphologic elements under examination being calculated from sections of diaphragmal muscles. A high larval metabolic rate was demonstrated in all periods examined. The isotope was also found to accumulate in the cuticle, less at 11 (greater permeability) than at 19 days or 8 weeks of infection. The penetration of high energy phosphorus compounds was mediated by the cyst formed round the parasite. The substance of the infected muscular fibre, contained, in the later period, between the cyst and the larval body, showed all the time an increased metabolic rate, higher than that of normal muscular fibres. Presumably, a significant role is played by the muscular fibre nuclei that not only persist but increase in size. In later phase of infection their P32 accumulation showed a higher percentage than that of normal fibres. The autoradiographic method has corroborated the data obtained with biochemical procedure and provided additional informations about localization of the occurring processes.
Przeprowadzone badania dotyczyły zmian w syntezie DNA, RNA i białek w komórkach jelita cienkiego myszy w warunkach długotrwałej, trzymiesięcznej intoksykacji kadmem i selenem oraz ustalenia, czy selen modyfikuje zmiany wywołane działaniem kadmu.
Background: The one of the most recent imaging technology is X-ray microtomography which allows non-invasive three-dimensional visualisation of structures. It also offers the opportunity to conduct a comprehensive quantitative analysis of the tested objects such as measuring the shares of the various phases, determining the material density and distribution of the size of pores and particles. The aim of the paper was to present an overview on the applicability and relevance of X-ray microtomography in the study of mineralised tissues of the teeth. Materials and methods: The article is based on the most recent and significant literature and own observations. Results: The use of X-ray microtomography in dentistry has recently increased and includes, inter alia, the assessment of the density of minerals in enamel and dentin, the detection of demineralisation in an artificially and a naturally induced caries, the automatic measurement of the depth of cavities in dentin, the measurement of the amount of removed dentin in preparation of carious lesions by various methods, the assessment of microleakage around fillings and fissure sealants, cortical bone density measurement, evaluation of root canal morphology, comparison of the accuracy of root canal working and filling by various methods. Conclusions: X-ray microtomography offers within the analysis of mineralised tissues — complex structures of bone, teeth and biomedical materials, turn out to be indispensable since it opens new opportunities for cognitive and implementation research. (Folia Morphol 2017; 76, 2: 143–148)
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