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1

Etylen a reakcje obronne roslin

100%
Handschuh L.
Biotechnologia
|
1999
|
nr 3
86-93
2

Mikromacierze DNA w diagnostyce medycznej

51%
Handschuh L., Magacz T., Figlerowicz M.
Biotechnologia
|
2009
|
nr 2
95-112
3

Quantitative analysis of yellow lupine csbp genes expression

51%
Molinska A.-N., Handschuh L., Wloszczak W.-M., Sikorski M.-M.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
4

Zastosowanie mikromacierzy DNA w genomice strukturalnej i funkcjonalnej

51%
Zmienko A., Handschuh L., Goralski M., Figlerowicz M.
Biotechnologia
|
2008
|
nr 4
39-53
5

Mikromacierze DNA - analiza danych

51%
Stepniak P., Handschuh L., Figlerowicz M.
Biotechnologia
|
2008
|
nr 4
68-87
6
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The methods of normalization used in the analysis of two-color microarrays

51%
Siatkowski I., Zyprych J., Handschuh L., Figlerowicz M.
Colloquium Biometricum
|
2009
|
tom 39
7

DNA microarray application for gebe expression profiling in plants

51%
Wloszczak W., Handschuh L., Figlerowicz M., Sikorski M. M.
Acta Biochimica Polonica
|
2006
|
tom 53
|
nr Supplement 1
8

RNAi-mediated gene silencing in legumes

51%
Handschuh L., Florek B., Sikorski M. M., Figlerowicz M.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
9

Structural and functional characteristics of two novel members of pathogenesis-related multigene family of class from yellow lupine

45%
Handschuh L., Femiak I., Kasperska A., Figlerowicz M., Sikorski M. M.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr 4
783-796
 PR-10 proteins (pathogensis-related), ubiquitous within the plant kingdom, are usually encoded by multigene families. To date we have identified 10 homologous pr-10 genes in a yellow lupine cDNA library. Here, the structure and expression of two newly identified yellow lupine pr-10 genes (LlYpr10-2b and LlYpr10-2f) are presented. Many potential regulatory sites were found in both gene promoters including common ones as well as those unique for each gene. However, promoter deletion analysis in transgenic tobacco plants revealed similar patterns of reporter gene (gus) expression. Shortened fragments of both gene promoters studied caused high GUS activity in leaves (along vascular bundles), stamen stigma, anthers and pollen grains. When conjugated with longer LlYpr-10.2 promoter fragments, GUS was additionally present in petal edges. Only a long fragment of the LlYpr10-2b gene promoter caused GUS expression in the stem. In yellow lupine the pr-10.2 genes are present in all studied organs, but their level of expression depends on the stage of development and is affected by wounding, oxidative stress and salicylic acid treatment. Silencing of the Llpr-10.2b gene in 4-week-old yellow lupine plants did not lead to any visible symptoms, which suggests that the function of the silenced gene is supplemented by its close homologues, still present in the studied plants.
10

Choroby rozrostowe krwi - analiza transkryptomu z zastosowaniem mikromacierzy DNA

45%
Handschuh L., Lewandowski K., Kazmierczak M., Komarnicki M., Figlerowicz M.
Biotechnologia
|
2009
|
nr 3
22-43
11

Mikromacierze DNA - zasady projektowania sond

45%
Formanowicz P., Urbaniak R., Handschuh L., Formanowicz D., Figlerowicz M.
Biotechnologia
|
2008
|
nr 4
54-67
12

The genetic landscape of pre-state Iron Ages societies of the East Central Europe

39%
Stolarek I., Handschuh L., Juras A., Marcinkowska-Swojak M., Kozlowski P., Figlerowicz M.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
13

Impact of DNA microarray data transformation on gene expression analysis - comparison of two normalization methods

39%
Schmidt M. T., Handschuh L., Zyprych J., Szabelska A., Olejnik-Schmidt A. K., Siatkowski I., Figlerowicz M.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 4
Two-color DNA microarrays are commonly used for the analysis of global gene expression. They provide information on relative abundance of thousands of mRNAs. However, the generated data need to be normalized to minimize systematic variations so that biologically significant differences can be more easily identified. A large number of normalization procedures have been proposed and many softwares for microarray data analysis are; available. Here, we have applied two normalization methods (median and loess) from two packages of microarray data analysis softwares. They were examined using a sample data set. We found that the number of genes identified as differentially expressed varied significantly depending on the method applied. The obtained results, i.e. lists of differentially expressed genes, were consistent only when we used median normalization methods. Loess normalization implemented in the two software packages provided less coherent and for some probes even contradictory results.In general, our results provide an additional piece of evidence that the normalization method can profoundly influence final results of DNA microarray-based analysis. The impact of the normalization method depends greatly on the algorithm employed. Consequently, the normalization procedure must be carefully considered and optimized for each individual data set.
14

Levels of NPM1 alternative transcripts in acute myeloid and lymphoblastic leukemia

33%
Handschuh L., Wojciechowski P., Kazmierczak M., Marcinkowska-Swojak M., Luczak M., Lewandowski K., Komarnicki M., Blazewicz J., Figlerowicz M., Kozlowski P.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
15

Half the costs keep the quality. Targeted enrichment for the Next Generation Sequencing of ancient genomes

33%
Marcinkowska-Swojak M., Stolarek I., Zenczak M., Handschuh L., Philips A., Juras A., Kozlowski P., Figlerowicz M.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
16

Bioinformatic construction of PUM1 and PUM2 RNA-Regulons in TCam-2 cell line

27%
Smialek M. J., Sajek M., Swiercz A., Ilaslan E., Janecki D. M., Kotecki M., Kusz-Zamelczyk K., Wozniak T., Handschuh L., Figlerowicz M., Jaruzelska J.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
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