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Antimicrobial activity of leaf and flowering cones of Abies cilicica ssp. cilicica (Pinaceae) crude extracts

100%
Saleh B., Al-Mariri A.
Herba Polonica
|
2016
|
tom 62
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nr 1
Introduction: Abies cilicica ssp. cilicica is an endemic species grown naturally in Syria. This taxon could be a potential and promising source of antimicrobial activity. Objective: Methanolic, ethanolic and acetonic extracts of leaf (LE) and flowering cones (FE) of A. cilicica ssp. cilicica plant were evaluated for their antimicrobial activity against 3 bacteria (Enterococcus faecalis, Proteus vulgaris and Klebsiella pneumoniae) and two fungi (Candida albicans and Aspergillus niger) pathogens. Methods: A. cilicica antimicrobial activity was evaluated by disc-diffusion method (zone of inhibitions-ZIs), activity index (AI) and minimum inhibitory concentrations (MICs) (Microdilution broth susceptibility assay). Similarly, ciprofloxacin (10 mg/ml) and nystatin (5 μg/ml) antibiotics were used as a standard for the antimicrobial activity of bacteria and fungi isolates, respectively. Results: Data revealed that the acetone FE with highest ZIs and lowest MICs showed the strongest antimicrobial activity against tested pathogens, while, ethanol LE was the lowest potent extract against all tested microorganisms. P. vulgaris bacteria was considered as the most sensitive isolate by showing the lowest MICs value of 0.52 μg/ml, whereas, A. niger fungus was pronounced as the most tolerant isolate by expressing the highest MICs value of 26.67 μg/ml. Conclusion: The current investigation proved the potential activity of A. cilicica extracts as an antibacterial and an antifungal source grown naturally in Syria.
2
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In vitro leaves and twigs antimicrobial properties of Psidium guajava L. (Myrtaceae)

80%
Saleh B., Al-Halab L., Al-Mariri A.
Herba Polonica
|
2015
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tom 61
|
nr 3
The current study aimed to investigate antimicrobial activity of methanol, ethanol, acetone, ethyl acetate and hot water (leaves and twigs fractions) Psidium guajava L. crude extracts against three bacteria and two fungi pathogens. Antimicrobial activity expressed by disc-diffusion assay (zone of inhibitions - ZIs), activity index (AI) and minimum inhibitory concentration (MICs) that were measured as reported in many investigations. Based upon the estimated ZIs, AI and MICs values, hot water twigs <1 cm diameter extract was the most potent against all tested microorganisms. The MICs value ranged between 4 and 7.2 mg/ml for bacteria, while, it was between 14.5 and 37.3 mg/ml for fungi. Moreover, ethyl acetate had the lowest antimicrobial activity compared to the other tested solvents. From the results obtained herein, it could be concluded that P. guajava serve as antibacterial and antifungal agent.
3
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Antimicrobial activity of Ficus sycomorus L. (Moraceae) leaf and stem-bark extracts against multidrug resistant human pathogens

80%
Saleh B., Hammoud R., Al-Mariri A.
Herba Polonica
|
2015
|
tom 61
|
nr 1
The present work was conducted to investigate antibacterial activity of methanol and acetone in leaf (LE) and stem-bark (SBE) of Ficus sycomorus L. crude extracts against sensitive and resistant species of Staphylococcus aureus and Acinetobacter baumannii pathogens. Antimicrobial activity expressed by disc-diffusion method (zone of inhibitions – ZIs), minimum inhibitory concentrations (MICs) and minimum bactericidal concentration (MBC) were measured as reported for many investigations. Similar study with 6 commercial antibiotics as a reference drug was undertaken. Based upon the estimated ZIs, MIC and MBC values, acetone LE exhibited higher antimicrobial activity than that of methanol one. Otherwise, standard antibiotics have lower effectiveness (ZIs, MICs and MBC) on all tested bacteria as compared to the SBE and LE. The highest antibacterial activity was recorded in sensitive A. baumannii isolate with MICs 2.5, 4.9 mg/ml and MBC 3.8, 9.7 mg/ml for acetone LE and SBE, respectively. Our data indicated that the lowest antibiotics antibacterial activity was recorded for resistant A. baumannii pathogen. It was lower than those of the both plant fractions extracts.
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