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Mycotoxins are secondary metabolites of fungi, which may cause diseases in animals or humans. Aflatoxin B₁ is mycotoxin, which is known to frequently contaminate poorly stored food products destinated for human consuption. In nature, there exist microorganisms for which the aflatoxins are non-toxic. Aflatoxins are degraded through the microorganism’s activity and the obtained products are probably utilized in their metabolism or the microorganisms have ability to bind aflatoxins to their surface. Components of herbs and spices have antiaflatoxigenic properties. They inhibit fungal development and subsequent aflatoxin production. The study reviews literature concerning the detoxification of mycotoxins by microorganisms and components of herbs and spices.
The present study investigated the effectiveness of three different disinfectants: preparation H1 (two-component preparation based on hydrogen peroxide); Pedox (multi-component preparation based on peroxyacetic acid) and Savo hypochlorite preparation) against Malassezia pachydermatis. The antifungal activity of disinfectants was tested by quantitative suspension method according to STN EN 1650. The results confirmed 100% effectiveness of these disinfectants at all concentrations and exposure times tested.
The aim of this study was to use the natural pigment produced by Monascus purpureus as a substitute for nitrites in the production of meat products. Two different concentrations of a Monascus purpureus extract (0.5 g.kg⁻¹ and 0.75 g.kg⁻¹) were tested and compared with the control sample (C) containing a nitrite salting mixture without any addition of Monascus purpureus extract. Based upon the results, poultry ham prepared with half the quantity of nitrite salting mixture and 0.5 g.kg⁻¹ of Monascus purpureus extract showed the most desirable colour, flavour and appearance, the best microbiological parameters and the most suitable salt content.
The goal of the work was to monitor the occurrence of helmintologic disorders of dogs in the district of Košice-surroundings. Moreover, the authors wished to evaluate the potential devitalising effect of the following selected chemical substances and disinfective agents: NaOH (5%, 70°C), Savo (10%), Saniten (10%), H1(100%) on eggs of T. canis under laboratory conditions. Using coprologic examination the authors realized that the most common eggs in dog feces are of T. canis (41.4%). Then follow eggs of Trichuris sp. (21.8%), T. leonina (11.5%), eggs from the Ancylostomatidae group (9.2%) and Capillaria sp. (2.3%). Under laboratory conditions, the ovocide effectiveness of NaOH on nonembryonated eggs of T. canis by an exposition of 180 minutes was 23.98 ± 4.33%. The disinfective agent SAVO also had low effectiveness; by an exposition of 180 minutes it devitalized 24.77 ± 5.33% of eggs of T. canis. A higher effectiveness was evidenced by the disinfective agent Saniten. Using the disinfective agent H1, 98.04 ± 1.77% eggs of T. canis were devitalized. Based on the testing of each agent under laboratory conditions the authors can recommend the usage of the disinfective agent Hviezda, as it evidenced the best devitalizing effect on the eggs of T. canis.
Bioprotective lactic acid from bacterial culture FloraCarn L-2u was tested for its biopreservative capacities to improve the quality and safety of cod salad, with additives: NaCl, acetic acid, sugar, mayonnaise, mustard and vegetables. The salad was divided into three portions: one untreated and two treated with a commercial Lactobacillus alimentarius biopreservative, inoculated in different concentrations (0.05 and 0.1g/kg). Lactic acid production, pH, development of the indigenous flora were examined for 14 days of aerobic storage in a refrigerator. The level of indigenous coliform bacteria, Staphylococcus aureus and moulds were reduced. The antibacterial effect of FloraCarn L-2u is attributed to lactic acid produced by this culture.
The composition of mycoflora in storage rooms, and other rooms in a poultry-processing plant, as well as on the surfaces of egg shells was observed. The concentrations of both aflatoxin B₁ and ochratoxin A were determined in the shell eggs at room temperature and humidity, at a higher temperature and humidity, and in the eggs previously contaminated by Aspergillus flavus. We found that there was a reciprocal correlation between the presence of microscopic filamentous fungi in the air and on the working tables (Cladosporium spp. 45.5%, Penicillium spp. 36.4%, Mucor spp. 9.0%). The penetration of mycotoxins through the egg shell was relatively low and the residue limit of aflatoxin B₁ allowed (5 μg.kg⁻¹) was not exceeded in any sample of egg tested. However, the residue limit of ochratoxin A (20 μg.kg⁻¹) was exceeded in one case.
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