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The hemagglutinating activity of Pseudomonas aeruginosa strains isolated from humans and animals

100%

Wolska K., Swiderska E., Jakubczak A.

Polish Journal of Veterinary Sciences

2006

tom 09

nr 2

The presence and type of adhesins occurring in Pseudomonas aeruginosa strains were determined by hemagglutination test with a 3% suspension of normal and trypsin-treated human group A erythrocytes, with or without the addition of sugar inhibitors (D-mannose, D-glucose, L-fucose, D-galactose, D-fructose, lactose, N-acetylneuraminic acid, N-acetylglucosamine and N-acetylgalactosamine). This study showed that a low percentage of Pseudomonas aeruginosa strains caused the agglutination of normal erythrocytes. Trypsin treatment of erythrocytes did not affect the hemagglutinating properties, indicating that hemagglutination was not dependent upon a trypsin-sensitive protein on the erythrocytes surface. Most of the studied strains agglutinated RBCs at 37°C. A great variability in the inhibiting activity on studied strains was observed among the carbohydrates tested. These results demonstrated the predominant role of N-acetylglucosamine, N-acetylgalactosamine and N-acetylneuraminic acid for Pseudomonas aeruginosa adhesion to RBCs.

Ograniczanie wyników

1 Polish Journal of Veterinary Sciences

1 Jakubczak A.

1 Swiderska E.

1 Wolska K.

1 2006

Wyniki wyszukiwania

The hemagglutinating activity of Pseudomonas aeruginosa strains isolated from humans and animals