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1

Safety science - quality improvement of Tussilago farfara via biotechnology

100%
Kopp B., Wawrosch C.
Herba Polonica
|
2007
|
tom 53
|
nr 2
2

The effect of transcutaneous nerve stimulation [TENS] on gastric electrical activity

71%
Furgala A., Thor P. J., Kolasinska-Kloch W., Kopp B., Laskiewicz J.
Journal of Physiology and Pharmacology. Supplement
|
2001
|
tom 52
|
nr 2
3
Content available remote

The effect of transcutaneous nerve stimulation (TENS) on gastric electrical activity

61%
Furgala A., Thor P. J., Kolasinska-Kloch W., Krygowska-Wajs A., Kopp B., Laskiewicz J.
Journal of Physiology and Pharmacology
|
2001
|
tom 52
|
nr 4,1
TENS became widely accepted method of treatment pain syndromes in clinical practice. Lately has been shown that its affects also gastrointestinal tract by releasing NANC neurotransmitter VIP. The aim of this study was to evaluate the effects of TENS on gastric myoelectric activities measured by electrogastrography (EGG). Eighteen healthy men (mean age 23 1.7) were included in the study. Healthy volunteers were divided on 3 groups each 6 persons: with normogastria occurring at 94.5 7% of recording time — group A, with predominant bradygastria (36.6 ± 14%) — group B and with tachygastria (33 14%) — group C. In fasted condition EGG (Synectics, Sweden) was recorded with skin electrodes. TENS 15 min was performed with use of Sinus 5 stimulator (6 Hz, 0.1ms duration, intensities 10 — 20mA, Zimmer, Germany). Stimulating electrodes were placed on non-dominant hand. Results: None of the subjects during TENS reported any side effects or symptoms, during the all studies. In group A in the fasting recordings, after TENS, an decrease of the normal values in the range 2 — 4cpm down to 78.5 ± 21% of recording time (p = 0.03) occurred. The dominant frequency in the bradygastric region increased up to 17.7 7% of the total recording. In group B TENS decreased bradygastria level from 36.6 14% to 20.6 15% (p = 0.02). TENS did not significantly affect tachygastria in group C. Amplitude of the EGG signal after TENS in group B and C increased by 40 and 150% respectively (p < 0.05). Significant decrease of the amplitude was observed in group A (13%). We conclude that TENS by activating centrally mediated somato-visceral reflexes affects gastric electrical activity. Our results suggest that TENS may be useful in treatment of the gastric dysrhythmia.
4

Effects of root colonization by symbiotic arbuscular mycorrhizal fungi on the yield of pharmacologically active compounds in Angelica archangelica L.

51%
Zitterl-Eglseer K., Nell M., Lamien-Meda A., Steinkellner S., Wawrosch C., Kopp B., Zitterl W., Vierheilig H., Novak J.
Acta Physiologiae Plantarum
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2015
|
tom 37
|
nr 02
The major effects of arbuscular mycorrhizal fungi (AMF) colonization on plant growth, monoterpenoids and coumarins were determined in two genotypes of angelica (Angelica archangelica L., Apiaceae). Genetically uniform experimental plants were used, which was achieved by in vitro propagation of two genotypes (individual plants). The effects of Glomus mosseae (Funneliformis mosseae, BEG 12), Glomus intraradices (Rhizophagus intraradices, BB-E) and the AMF mixture Symbivit were tested against a negative (without AMF and without additional phosphorus) and a positive (KH2-PO4 without AMF) control in five plants per treatment. All in all 50 plants were investigated. Fifteen monoterpenoid and seven coumarin compounds were quantitatively determined in the rhizome and coarse root fractions by means of GC/MS using an internal standard. The sum of the monoterpenoids and coumarins was calculated. Concentrations of compounds rather slightly decreased upon inoculation with AMF when compared to the control. In contrast, biomass increased showing the highest amounts for plants treated with G. mosseae. These results finally caused a marked increase in yield for G. mosseae treated plants compared to the control. Phosphorus treatment led to the lowest yield with significant results for coumarins and in particular for osthole compared to G. mosseae treatment. This is also evidence of an independent effect from an increased phosphorus uptake due to root colonization by AMF. In summary, the results showed a marked increase in yield of all investigated compounds through treatment with G. mosseae compared to the control group, even if the level of significance was just barely missed presumably on account of the small sample size.
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