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Biological activity of benzene and its metabolites of Chlorella vulgaris cultures in fibroblasts cultures from mouse liver

100%

Wardas M., Wardas W., Majnusz U.

Polish Journal of Environmental Studies

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1992

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tom 01

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nr 2

Our objective was the assessment of algal medium harmfulness for in vitro fibroblasts. The algal medium was from Chlorella cultures (Beijerinck 1890) grown in the presence of benzene, which was added before the inoculation of culture. The medium contained, besides the benzene residues, its various metabolites formed during culture, among them phenol and catechol. Its addition to fibroblast cultures resulted in a decrease in their growth intensity and protein content in fibroblasts as well as an increase in DNA content and 14C-thymidine incorporation intensity to fibroblasts. The latter effect was probably connected with the inhibition of cell-division and DNA damage reparations. The obtained results indicate that Chlorella algae, besides other hydrobionts, take part in the forming of the benzene-like pollution toxicity in water habitat.

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The effect of benzene, phenol and catechol an the run of the logarithmic phase of growth of Chlorella vulgaris, Beijerinck 1890 cultures

100%

Wardas W., Wardas M., Majnusz U., Sochacka J.

Polish Journal of Environmental Studies

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1995

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tom 04

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nr 3

We estimated the toxic effect of benzene, phenol, and catechol on the logarithmic phase of growth of Chlorella vulgaris, Beijerinck 1890 cultures; the compounds show various degrees of toxicity to the algae. A concept was developed concerning each compound's toxicity index EC50/(T2-T1) The concept assumes the calculation of the index as well as benzene, phenol, and catechof-ináuced inhibition of test cultures growth f(T2-T1,) in T2-T1, interval which corresponds in our experiment to the logarithmic phase of control culture growth. f(T2-T1,) was proved to remain in linear relation to the initial concentration of the investigated substances in culture medium. Also, it was found that the values of EC50/(T2-T1), calculated by probit methods, are characterized by confidence intervals comparable to those determined for EC50/96 and EC50/Tmax (the indexes were determined at hour 96 and Tmax, respectively)

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Toksyczność wybranych węglowodorów względem glonów Chlorella w hodowlach laboratoryjnych

88%

Wardas W., Wardas M., Mazurek U., Waszkiewicz E., Majnusz U.

Roczniki Państwowego Zakładu Higieny

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1987

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tom 38

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nr 6

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The toxicity of benzene, phenol and catechol related to Chlorella vulgaris, Beijerinck 1980 in laboratory cultures under various conditions

88%

Majnusz U., Wardas W., Sochacka J., Pawlowska-Goral K., Lipska I.

Polish Journal of Environmental Studies

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1994

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tom 03

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nr 3

An inhibition of growth of Chlorella vulgaris, Beijerinck 1980 laboratory cultures carried out under different conditions caused by benzene, phenol and catechol was investigated. Each of the investigated substances was added to the culture medium at 4 or 5 different concentrations, directly before inoculation. The cultures were carried up to the stationary phase of growth. The density of alga cell suspension in the culture medium was determined with the use of a Bürker hemocytometer as well as EC50/96 and EC 50/Tmax values. It was stated that under some conditions it was possible to estimate the toxicity of the investigated substances, either in open cultures (flasks with microbiological corks, type I cultures) or closed (flasks with ground corks, type II cultures), but in this second case with the presence of NaHCO3 as an additional source of C02. The results presented in this paper indicate a relatively high toxicity of benzene and its investigated metabolites, phenol and catechol, as well as its dependence on conditions in the investigated cultures.

Ograniczanie wyników

3 Polish Journal of Environmental Studies

1 Roczniki Państwowego Zakładu Higieny

4 Majnusz U.

4 Wardas W.

3 Wardas M.

2 Sochacka J.

1 Lipska I.

1 Mazurek U.

1 Pawlowska-Goral K.

1 Waszkiewicz E.

1 1995

1 1994

1 1992

1 1987

Biological activity of benzene and its metabolites of Chlorella vulgaris cultures in fibroblasts cultures from mouse liver

The effect of benzene, phenol and catechol an the run of the logarithmic phase of growth of Chlorella vulgaris, Beijerinck 1890 cultures

Toksyczność wybranych węglowodorów względem glonów Chlorella w hodowlach laboratoryjnych

The toxicity of benzene, phenol and catechol related to Chlorella vulgaris, Beijerinck 1980 in laboratory cultures under various conditions