Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 10

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
The influence of time and storage conditions on the stability of specific antibodies against EBLV was determined. The titer of antibodies was tested by use of the immunodiffusion test and ELISA. The sera in the lyophilized form with the addition of 0.01% sodium azide and 0.01% merthiolate were stored at + 4°C, — 20°C and at — 20°C after freezing and thawing. Sera with the above preparations stored at + 4°C were tested every three days for three weeks and the remaining sera every months for one year. The titers of the specific antibodies did not change their values in sera stored at + 4°C for 21 days, at — 20°C for 6 months. The most significant changes in serum titers were observed after several thawings and refrigerations.
Using the PCR method the proviral sequence of the bovine leukaemia virus was detected in the peripheral blood leukocytes of cattle from an infected herd. BLV antibodies were determined by the ELISA and AGID tests. PCR amplification was performed with one set of 20-mer oli- gonucleotyde primers that should produce a 364 bp fragment of BLV-DNA located in the gag gene region. The reaction products were analyzed in 1 A°/< agarose, blatted to Hybond-N filter by the Southern method and probed with an 8.3 kb Sac I fragment of BLV, labelled with di- goxigenin -dVTP. In 23 animals examined by PCR the presence of proviral DNA was found in all serologically positive animals and also in three serologically negative animals. BLV-DNA was not detected in only one seropositive individual. The presented findings indicate that the determination of the proviral DNA of BLV using the PCR method is more sensitive than serological tests and it should be beneficial for the diagnosis of cattle infection with BLV.
Тестами ID и ELISA определяли появление антител против вируса энзоотического лейкоза крупного рогатого скота в сыворотке крови 28 новорожденных телят, которых поили молозивом зараженных коров. У 25 телят положительные реакции показались результатом присутствия антител молозива, которые удерживались максимально до б месяца после рождения, хотя у 70% телят они исчезали через 3—4 месяца. После затухании антител молозива у 3 телят этой группы доказали присутствие эндогенных антител между 202—248 днями после рождения. У 3 телят, зараженных внутриматочно, антитела присуствовали песь период наблюдения. Во втором эксперименте тестом ELISA определяли антитела сыворотки крови 516 телят. У телят в возрасте 3 месяцев констатировали их у 5,9% (8/136), а у 6-месячных телят у 10,5% (40/380). Дискутируют возможность массового исследования телят в возрасте 3—4 месяцев, изоляции положительных реагентов и повторного исследования в возрасте 5—6 месяцев и 8—9 месяцев.
The present studies were aimed at determining the relation between the findings obtained by means of serological tests and the specific molecular probe. Serological tests were performed according to the methods recommended by the Polish Ministry of Agriculture; ELISA was run with „Bioveta” and „Rhone Merieux” kits and the AGID test was performed with EBL antigen made in our laboratory. The molecular probe was prepared from the previously cloned provirus DNA of EBL virus. The EBL provirus was detected in 28 samples taken from 44 randomly selected cows in three herds on which a leukaemia eradication programme was in process. Three sera out of 28 positive reacting animals were negative in AGID test and only one serum in ELISA. The results indicate that the use of a specific molecular probe has some advantages in the diagnosis of latent virus infections. Besides, it can be applied in the studies on the pathogenesis of enzootic bovine leukaemia.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.