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Expression of bone morphogenetic proteins-2/4 in neural stem cells and their lineages

100%

Lu H.-Z., Hu J.-G.

Acta Neurobiologiae Experimentalis

2009

tom 69

nr 4

Neural stem cells (NSCs) are multipotent progenitor cells that possess the ability to self-renew and generate different neural cell types. Bone morphogenetic proteins (BMPs) play critical roles in the determination of the fate of NSCs. Although some reports delineate BMP expression in the early developing central nervous system (CNS), they deal mainly with its expressions in restricted areas. However, little information is available for BMP expression in NSCs and their lineages. In this study, based on an in vitro model in which NSCs can self-renew and differentiate into astrocytes, neurons or oligodendrocytes, we mimicked the environment of NSC development in vivo. Using an antibody which can recognize BMP-2 and BMP-4, we showed that BMP-2/4 is only expressed in astrocytes. Inflammatory cytokines (tumor necrosis factor-α and/or interferon-γ) do not influence the expression pattern of BMPs in NSCs and their lineages. These results suggest that astrocytes might be one source of BMPs during the differentiation of NSCs and in the inflammatory environment after CNS injury.

Activation of PKCα is required for migration of C6 glioma cells

61%

Hu J.-G., Wang X.-F., Zhou J.-S., Wang F.-C., Li X.-W., Lu H.-Z.

Acta Neurobiologiae Experimentalis

2010

tom 70

nr 3

The PKC signaling pathway has been implicated in diverse cellular functions. Here, we sought to investigate the role of PKCα/β in C6 glioma cell migration. We found that both PKCα and PKCβ were expressed by C6 glioma cells, but only PKCα was markedly activated in serum-treated C6 cells. Gö6976, a PKC α/β specific inhibitor, was found to cause a dose-dependent reduction of PKCα activation and cell migration induced by serum in C6 cells. These results collectively indicated that the PKCα signaling pathway is necessary for glioma cell migration. Our findings may provide an insight into a better understanding to the malignant progression of gliomas.

Ograniczanie wyników

2 Acta Neurobiologiae Experimentalis

2 Hu J.-G.

2 Lu H.-Z.

1 Li X.-W.

1 Wang F.-C.

1 Wang X.-F.

1 Zhou J.-S.

1 2010

1 2009

Wyniki wyszukiwania

Expression of bone morphogenetic proteins-2/4 in neural stem cells and their lineages

Activation of PKCα is required for migration of C6 glioma cells