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1

Effect of enterocin 4231 in Slovak fermented salami Puchov after its experimental inoculation with Listeria innocua Li1

100%
Laukova A., Turek P.
Acta Scientiarum Polonorum. Technologia Alimentaria
|
2011
|
tom 10
|
nr 4
Background. Enteroccocci occur and may compete well in fermented sausages and Enterococcus faecium represents that species of the lactic acid bacteria which can be found in the fermented sausages. The representatives of this species can produce bacteriocins with predominant anti-listerial effect. Therefore, the effect of enterocin (Ent) 4231 produced by Enterococcus faecium CCM 4231 strain with probiotic properties was tested in a dry fermented salami Puchov (Slovak product) experimentally inoculated with L. innocua Li1 strain (107 cfu/ml). Material and methods. The bulk salami mixture was prepared in the pilot plant and 2.5 kg for each of three trials were transferred to the laboratory for the experiments. Three independent trials were conducted, each comprising then five salami samples (0.500 g). Trial A (reference control) involved only untreated salami mixture. Trial B represented salami mixture inoculated with Listeria innocua Li1 (107 cfu/ml). For trial C, Ent 4231 possessing activity 6400 AU/ml was added into the salami mixture inoculated with L. innocua Li1 (LilEnt). The mixtures were stuffed into collagen casings and the fiat shape salamis were transferred back to the pilot plant and treated according to conditions typical for this product and stored for 4 weeks. Results. The initial number of L. innocua Li1 in the inoculated salami mixture was 104 cfu/ml. Aft er Ent 4231 addition, the count of Listeria detected in the salami samples inoculated with Li1 and treated with Ent 4231 was 3.64 ±0.14 cfu/ml; difference 0.40 logarithmic cycles was noted between Li samples and Li /Ent samples. On day 2, the difference 1.86 log cycles was noted between Li1 and IM Ent samples. Although, in weeks 3 and 4, slight increase in Li1 cells was determined in Li salamis, the difference in the detection of Li1 cells in Li salamis and Li/Ent samples was even higher than that immedially after Ent addition (difference 2.30; 2.48 log cycles). Bacteriocin activity itself was not recovered from Li/Ent salamis. The pH of the all salamis was almost at the same level. Water activity and water content were not influenced. Conclusion. Addition of Ent 4231 during processing of salami Puchov experimentally inoculated with L. innocua Li1 has lead to decrease of Li1 cell growth, although the bacteriocin activity of Ent itself was not possible to detect in salami samples. The pH value, water activity, as well as sensory character of the final products were not negatively influenced.
2
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Escherichia coli strains from ostriches and their sensitivity to antimicrobial substances

100%
Scerbova J., Laukova A.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 2
Ostriches are bred especially for their high-quality meat. There is a lack of knowledge concerning the ostrich’s microflora. Escherichia coli is a commensal microorganism of the poultry intestine, ostriches included. However, some strains may become pathogenic. This study was therefore undertaken to detect coliform bacteria in ostrich faeces and to test their antibiotic profile and sensitivity to enterocins. Faeces (n=54, 18 mixture samples from 3 different age groups of 140 ostriches) were sampled to isolate coliform bacteria. The counts of coliform bacteria varied from 5.69 ± 2.4 log10 CFU/g to 5.73 ± 2.4 CFU/g. Pure colonies were identified using MALDI-TOF MS mass spectrometry and confirmed by phenotypization. Seventy-one strains were allotted to the species E. coli. Sixty-four of those 71 strains caused hemolysis. They were mostly polyresistant to antibiotics. Thirty-two poly-resistant strains of E. coli were sensitive to enterocins. These strains were most sensitive to Ent 9296 (26 strains). Moreover, Ent EM41 produced by E. faecium EM41 (isolated from ostrich faeces) inhibited the growth of 20 strains, reaching activity of 100 AU/ml. Our results indicate the possibility of enterocins being used for prevention/reduction of coliforms. Of course, in vivo studies are also being processed.
3

Use of bacteriocin preparation with anti-microbial activity in Saint-Paulin cheese

100%
Laukova A., Vlaemynck G.
Bulletin of the Veterinary Institute in Puławy
|
2003
|
tom 47
|
nr 2
4

Isolation of faecal Enterococcus faecium strains from rabbits and their sensitivity to antibiotics and ability to bacteriocin production

100%
Simonova M., Laukova A.
Bulletin of the Veterinary Institute in Puławy
|
2004
|
tom 48
|
nr 4
5

Antibiotic resistance of lactic acid bacteria from canine faeces

100%
Strompfova V., Laukova A.
Bulletin of the Veterinary Institute in Puławy
|
2004
|
tom 48
|
nr 3
6

A note on the influence of microgravity on the microbial endoecosystem of Japanese quail

81%
Laukova A., Boda K., Kmet V.
Journal of Animal and Feed Sciences
|
1993
|
tom 01
|
nr 3-4
295-301
7

Testing of probiotic and bacteriocin-producting lactic acid bacteria towards Eimeria sp.

81%
Strompfova V., Laukova A., Marcinakova M., Vasilkova Z.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 2
8
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Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

81%
Kandricakova A., Laukova A., Strompfova V.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 3
With an increasing number of pheasants as gamebirds being reared each year, these species are becoming a more prominent part of the workload of many veterinary practices. Only limited information can be found concerning the microflora of common pheasants. A significant part of the obligate microflora consists of lactic acid bacteria, including enterococci. In this study, faeces were sampled from 60 pheasants aged 16-17 weeks. Enterococcal counts reached 5.48±1.9 (log10) CFU/g. Strains (17) were taxonomically classified to the genus Enterococcus using the Maldi-Tof identification system; they were allotted to the species E. hirae (58.8%), E. faecium (23.5%) and E. faecalis (17.7%) by highly probable species identification or by secure genus identification/probable species identication. Species allocation was also confirmed using conventional biochemical tests. Most strains formed β-hemolysis. Gelatinase active phenotype was found in three E. faecalis strains. Enterococci were β-glucuronidase negative, mostly trypsin negative with slight or moderate production of α-chymotrypsin. EH52b and EF42 strains possessed the highest potential for pathogenicity. Average value of lactic acid was 1.78±0.33 mmo/L. Most strains were tetracycline resistant (82.4%). Polyresistant E. faecalis strains with positive gelatinase phenotype and possessing virulence factor genes confirmed using PCR (gelE, efaAfs, ccf cob, cpd) were sensitive to enterocins (activity 1600-25 600 AU/mL).
9
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Assessment of biofilm formation by faecal strains of Enterococcus hirae from different species of animals

81%
Bino E., Laukova A., Kandricakova A., Nemcova R.
Polish Journal of Veterinary Sciences
|
2018
|
tom 21
|
nr 4
10
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Domestic fowl of ducks, a source of faecal bioactive Enterococcus hirae strains

81%
Laukova A., Bino E., Scerbova J.
Polish Journal of Veterinary Sciences
|
2019
|
tom 22
|
nr 3
11

Occurrence of structural enterocin genes among silage enterococci

81%
Marcinakova M., Simonova M., Strompfova V., Laukova A.
Bulletin of the Veterinary Institute in Puławy
|
2005
|
tom 49
|
nr 4
12

Effect of enterocin a on the intestinal epithelium of Japanese quails infected by Salmonella Duesseldorf

81%
Cigankova V., Laukova A., Guba P., Nemcova R.
Bulletin of the Veterinary Institute in Puławy
|
2004
|
tom 48
|
nr 1
13
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Distribution of CD4 and CD8 T cells in the small intestine of mice after probiotic treatment and Trichinella spiralis infection

71%
Dvoroznakova E., Buckova B., Hurnikova Z., Revajova V., Laukova A.
Annals of Parasitology
|
2016
|
tom 62
|
nr Suppl.
14

Hygiene of pasteurized milk depending on psychrotrophic microorganisms

71%
Burdova O., Baranova M., Laukova A., Rozanska H., Rola J. G.
Bulletin of the Veterinary Institute in Puławy
|
2002
|
tom 46
|
nr 2
15

Effect of potential probiotic activity of Enterococcus faecium EE3 strain against Salmonella infection in Japanese quails

71%
Marcinakova M., Strompfova V., Boldizarova K., Laukova A., Gancarcikova S.
Bulletin of the Veterinary Institute in Puławy
|
2004
|
tom 48
|
nr 4
16

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

61%
Sevcikova Z., Blanar J., Laukova A., Revajova V., Strompfova V., Levkut M.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 3
17
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Sensitivity to antimicrobials of faecal Buttiauxella spp. from roe and red deer (Capreolus capreolus, Cervus elaphus) detected with MALDI-TOF mass spectrometry

61%
Laukova A., Pogany Simonova M., Kubasova I., Miltko R., Belzecki G., Strompfova V.
Polish Journal of Veterinary Sciences
|
2018
|
tom 21
|
nr 3
18

Staphylococcal species detected in free-living trouts of East Slovakian water sources and their relation to antimicrobials

61%
Laukova A., Kandricakova A., Strompfova V., Chacornac J. P., Leroy S., Zitnan R.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 2
The aim of the study was to isolate and identify staphylococci from the intestinal samples of 24 trouts from East Slovakian waters. Moreover, their relation to antimicrobials was tested. The count of staphylococci in the trouts reached in average 4.0 × 10¹ colony forming units per gram. Twenty-two strains were identified by validated species-specific oligonucleotide array targeting the manganese-dependent superoxide dismutase-sodA gene. The identified strains were allotted to five species (Staphylococcus warnen, S. haemolyticus, S. epidermidis, S. hominis, S. pasteuri) clustered to three groups according to 16S rRNA sequences (S. epidermidis group, S. haemolyticus group, S. warneri group). These species belong to coagulase-negative staphylococci. All strains were sensitive to eight antibiotics out of 14 tested; the majority of strains were also sensitive to the remaining six antibiotics with the inhibitory zones from 13 to 41 mm. The strains were also sensitive at least to three enterocins of nine tested. Strains SW24/2, SHo 19/2, SHo20/l, SP19/1 were sensitive to eight of nine enterocins. All strains were sensitive to Ent A, P=EK13, and Ent EM41 with activity 100-6400 AU/mL. Strains SHo19/2 and SP19/1 were sensitive to Ent 2019 with activity up to 25600 AU/mL.
19

Polymerase chain reaction detection of caecal bacteria in case of preventive application of Enterococcus faecium EK13 against Salmonella enterica subsp. Enteritidis in chickens

61%
Herich R., Laukova A., Strompfova V., Revajova V., Levkut M., Pistl J.
Journal of Animal and Feed Sciences
|
2005
|
tom 14
|
nr 1
20
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Combined administration of bacteriocin-producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits

51%
Simonova M. P., Laukova A., Chrastinova L., Placha I., Strompfova V., Cobanova K., Formelova Z., Chrenkova M.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 4
The effect of Enterococcus faecium CCM7420 (EF) – enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.
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