Antioxidant activity (AA) of Lisosan G, a food supplement obtained from wheat grains, was evaluated by using the LOX/RNO, ORAC and TEAC methods. Very high AA was found, associated with a high fraction of freely-extractable phytochemicals expected to be readily absorbed in the small intestine and to exert systemic healthy effects. So, a possible beneficial effect of Lisosan G against non-alcoholic hepatic steatosis induced in mice by 90 days of hyperlipidic diet was studied. Feeding Lisosan G to mice with hepatic steatosis caused rapid reversion of liver disorder: just after 7 days the liver resulted free of steatosis, showing triglyceride content and histological properties similar to control group. Contrarily, the switch of hyperlipidic diet towards a standard diet did not rapidly reverse hepatic steatosis, with a recovery observed only after 30 days. Our results shed a first insight on the therapeutic potential of Lisosan G against the hepatic steatosis disorder in animal species.
In order to assess the quality and performance of bagged broccoli-raab, a recently marketed product, several nutritive parameters were determined in novel hybrid and conventional cultivars at pre- and post-packaging stages in the industrial environment. The characterization of shoots and composing organs at post-cut stage included contents of dietary fibre (DF), glycaemic carbohydrates (GC), antioxidant compounds (ACC) and capacity (AOC), which were determined by chromatographic methods and spectrophotometric assays. ACC and AOC were analysed during shelf life of bagged products. Genotype and storage effects were addressed as variability factors at fixed packaging conditions. Contents of DF and GC (39.64–34.57; 7.56–2.21 g/100 g), glucosinolates (37.47–24.63 mg/g SIN), and ACC (total phenolics: 18.64–14.92 mg GAE/g; flavonoids: 34.74–30.96 mg/g CE; flavonols: 14.62–14.08 mg QE/g), and AOC (Oxygen Radical Absorbance Capacity: 354.62–293.25 μmol/g TE; DPPH• scavenging activity: 59.35–46.14) were lower in shoots of the hybrid than marketed cultivar. In both genotypes, AOC was maximal in leaves, followed by florets and stems. The integrated analyses suggested that the hybrid genotype was better suited for fresh consumption and that increased ratio of florets/leaves vs. stem is expected to raise product antioxidant properties. The comparison of unprocessed and bagged products pointed at a value decay of most parameters except for glucosinolates and correlation analyses supported the necessity of performing multiple antioxidant assays to enhance product quality evaluation. As for shelf life, storage time was the major factor affecting antioxidant properties, while genotype and interaction effects were minimal.
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