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Composition of volatile in micropropagated and field grown aromatic plants from Tuscany Islands

100%

Pistelli L., Noccioli C., D`Angiolillo F., Pistelli L.

Acta Biochimica Polonica

2013

tom 60

nr 1

Aromatic plant species present in the natural Park of Tuscany Archipelago are used as flavoring agents and spices, as dietary supplements and in cosmetics and aromatherapy. The plants are usually collected from wild stands, inducing a depletion of the natural habitat. Therefore, micropropagation of these aromatic plants can play a role in the protection of the natural ecosystem, can guarantee a massive sustainable production and can provide standardized plant materials for diverse economical purposes. The aim of this study is to compare the volatile organic compounds produced by the wild plants with those from in vitro plantlets using headspace solid phase micro-extraction (HS-SPME) followed by capillary gas-chromatography coupled to mass spectrometry (GC-MS). Typical plants of this natural area selected for this work were Calamintha nepeta L., Crithmum maritimum L., Lavandula angustifolia L., Myrtus communis L., Rosmarinus officinalis L., Salvia officinalis L. and Satureja hortensis L. Different explants were used: microcuttings with vegetative apical parts, axillary buds and internodes. Sterilization percentage, multiplication rate and shoot length, as well as root formation were measured. The volatile aromatic profiles produced from in vitro plantlets were compared with those of the wild plants, in particular for C. maritimum, R. officinalis, S. officinalis and S. hortensis. This study indicated that the micropropagation technique can represent a valid alternative to produce massive and sterile plant material characterised by the same aromatic flavour as in the wild grown plants.

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Establishment of highly efficient Agrobacterium rhizogenes-mediated transformation for Stevia rebaudiana Bertoni explants

100%

Michalec-Warzecha Z., Pistelli L., D'Angiolillo F., Libik-Konieczny M.

Acta Biologica Cracoviensia. Series Botanica

2016

tom 58

nr 1

Leaves and internodes from Stevia rebaudiana Bertoni plants growing in different conditions were used for transformation with two strains of Agrobacterium rhizogenes: ATCC 15384 and LBA 9402. Hairy roots formation was observed and the percentage of the transformed explants depended on the type of explant, time of inoculation and inoculum concentration. Inoculation of explants from ex vitro and in vitro plants with LBA 9402 strain led to higher efficiency of transformation than inoculation with ATCC 15384 strain. Growth rate of hairy roots in liquid culture was assessed under light and dark conditions. It was found that the growth of hairy roots decreased significantly under light conditions. Transformation of hairy roots growing in different culture conditions was confirmed at the molecular level using PCR method with primers constructed against rolB and rolC genes from A. rhizogenes.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Acta Biologica Cracoviensia. Series Botanica

2 Pistelli L.

1 D'Angiolillo F.

1 D`Angiolillo F.

1 Libik-Konieczny M.

1 Michalec-Warzecha Z.

1 Noccioli C.

1 2016

1 2013

Wyniki wyszukiwania

Composition of volatile in micropropagated and field grown aromatic plants from Tuscany Islands

Establishment of highly efficient Agrobacterium rhizogenes-mediated transformation for Stevia rebaudiana Bertoni explants