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1

In vitro hormone-regulated growth and floral induction of Cuscuta reflexa: a parasitic angiosperm

100%
Das P., Kar M., Sahoo S.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 3
The role of different growth regulators in callus induction, shoot regeneration, floral induction and chlorophyll content of the obligatory parasitic plant Cuscuta reflexa has been studied. Callus development was excellent from the nodal part of the shoot explants in modified Murashige and Skoog (MMS) media supplemented with 2 mg L⁻¹ benzyl adenine (MMS1c). Supplementation of 2 mg L⁻¹ naphthalene acetic acid (NAA) along with MMS1c (MMS2c) was responsible for estimable shoot induction and development in callus. 2,4-Dichloro acetic acid (2,4-D) played a crucial role in the floral induction of C. reflexa in vitro. MMS supplemented with 2 mg L⁻¹ NAA and 2 mg L⁻¹ 2,4-D (MMS3b) supported floral induction after shooting in vitro. MMS supplemented with 3 mg L⁻¹ 2,4-D (MMS4a) rapidly induced flower directly from the stem explants without showing any elongation of shoot. MMS1c along with MMS3b (MMS5a) showed callus proliferation followed by shoot elongation and floral induction. In vitro MMS5a grown plants show a sharp increase in the chlorophyll contents. Cytokinin treatment further increases the chlorophyll level of the plant.
2

Temporal variation of metal concentrations of creek sediment samples

100%
Duman F., Urey E., Kar M.
Polish Journal of Environmental Studies
|
2013
|
tom 22
|
nr 5
The aim of our study was to investigate the changes of metal concentrations in stream sediments depending on station and sampling period variation. Sediment samples were collected seasonally from six separate stations selected along Kilicozu Creek (Kirsehir, Turkey), and the Pb, Cr, Cu, Ni, Zn, and Cd concentrations were determined. Stations were selected on the basis of pollution gradient. It was observed that the metal concentration means per annum were Zn> Cr> Ni> Cu> Pb> Cd. Seasonal highest values of heavy metals were observed as follows; Pb (14.4 µg·g⁻¹), Ni (43 µg·g⁻¹), and Cd (6.2 µg·g⁻¹) in autumn, Cr (55.7 µg·g⁻¹) and Zn (71.9 µg·g⁻¹) in summer, and Cu (42.5 µg·g⁻¹) in spring. One-way ANOVA results also showed that there were significant metal concentration changes between stations. Potential metal contamination risk was determined for studied sediment samples. It was observed that Cd contamination exceeded the limit values in this stream sediment. According to the reference values, Zn or Pb contamination in the creek sediment has not reached the effective level. The findings of this study may be useful for further biomonitoring studies.
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