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In recent years, the need for food safety and animal welfare has stimulated the search for new feeding strategies based on the use of natural feed additives, capable of ensuring the health of animals and excellence in their production performance. This study evaluates the phytochemical composition, antioxidant activity, and anti-hemolytic effects of a new product, Lisosan Reduction, containing the grain lysate Lisosan G and extracts of Picrorhiza and Desmodium. The data obtained were compared with those related to Lisosan G alone. Phytochemical compounds are present in high quantities in both products. The flavonoids, in particular, are doubled in Lisosan Reduction compared to Lisosan G. Lisosan Reduction also showed a greater antioxidant capacity, determined by the ORAC test, compared to Lisosan G. The antioxidant activity of Lisosan G and Lisosan Reduction was also evaluated in an ex-vivo system of human and dog erythrocytes (CAA-RBC). The results of this test show a higher antioxidant effect of the mixture Lisosan Reduction, compared to the lysate alone, both in human and canine samples, with greater effectiveness in human erythrocytes. The anti-hemolytic effects of Lisosan G and Lisosan Reduction were also evaluated, and the results show a stronger effect of Lisosan Reduction, compared with Lisosan G, in both human and dog samples. The results of our study demonstrate that Lisosan G and, in particular, Lisosan Reduction contain several bioactive compounds and have a strong antioxidant activity, suggesting the use of these compounds as feed additives to improve animal health.
Antioxidant activity (AA) of Lisosan G, a food supplement obtained from wheat grains, was evaluated by using the LOX/RNO, ORAC and TEAC methods. Very high AA was found, associated with a high fraction of freely-extractable phytochemicals expected to be readily absorbed in the small intestine and to exert systemic healthy effects. So, a possible beneficial effect of Lisosan G against non-alcoholic hepatic steatosis induced in mice by 90 days of hyperlipidic diet was studied. Feeding Lisosan G to mice with hepatic steatosis caused rapid reversion of liver disorder: just after 7 days the liver resulted free of steatosis, showing triglyceride content and histological properties similar to control group. Contrarily, the switch of hyperlipidic diet towards a standard diet did not rapidly reverse hepatic steatosis, with a recovery observed only after 30 days. Our results shed a first insight on the therapeutic potential of Lisosan G against the hepatic steatosis disorder in animal species.
In order to assess the quality and performance of bagged broccoli-raab, a recently marketed product, several nutritive parameters were determined in novel hybrid and conventional cultivars at pre- and post-packaging stages in the industrial environment. The characterization of shoots and composing organs at post-cut stage included contents of dietary fibre (DF), glycaemic carbohydrates (GC), antioxidant compounds (ACC) and capacity (AOC), which were determined by chromatographic methods and spectrophotometric assays. ACC and AOC were analysed during shelf life of bagged products. Genotype and storage effects were addressed as variability factors at fixed packaging conditions. Contents of DF and GC (39.64–34.57; 7.56–2.21 g/100 g), glucosinolates (37.47–24.63 mg/g SIN), and ACC (total phenolics: 18.64–14.92 mg GAE/g; flavonoids: 34.74–30.96 mg/g CE; flavonols: 14.62–14.08 mg QE/g), and AOC (Oxygen Radical Absorbance Capacity: 354.62–293.25 μmol/g TE; DPPH• scavenging activity: 59.35–46.14) were lower in shoots of the hybrid than marketed cultivar. In both genotypes, AOC was maximal in leaves, followed by florets and stems. The integrated analyses suggested that the hybrid genotype was better suited for fresh consumption and that increased ratio of florets/leaves vs. stem is expected to raise product antioxidant properties. The comparison of unprocessed and bagged products pointed at a value decay of most parameters except for glucosinolates and correlation analyses supported the necessity of performing multiple antioxidant assays to enhance product quality evaluation. As for shelf life, storage time was the major factor affecting antioxidant properties, while genotype and interaction effects were minimal.
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