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1

Rapd analysis of the somatic hybrids Solanum bulbocastanum(+)S.Tuberosum

100%
Boltowicz D., Szczerbakowa A., Wielgat B.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
2

Cytogenetic characteristics of the interspecific somatic hybrids of Solanum villosum (+) S. tuberosum

100%
Tarwacka J., Szczerbakowa A., Wielgat B.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
|
tom 51
|
nr 1
3

Interspecific somatic hybrids Solanum bulbocastanum [plus] S.tuberosum H-8105

100%
Szczerbakowa A., Boltowicz D., Wielgat B.
Acta Physiologiae Plantarum
|
2003
|
tom 25
|
nr 4
4

Molecular analysis of somatic hybrids between Solanum bulbocastanum and Solanum tuberosum

100%
Boltowicz D., Szczerbakowa A., Wielgat B.
Polish Journal of Natural Sciences. Supplement
|
2003
|
tom 01
5

Plant regeneration from the protoplasts of Solanum tuberosum, S.nigrum and S.bulbocastanum

100%
Szczerbakowa A., Borkowska M., Wielgat B.
Acta Physiologiae Plantarum
|
2000
|
tom 22
|
nr 1
6

RAPD analysis of the interspecific somatic hybrid Solanum bulbocastanum [plus] S. tuberosum

100%
Boltowicz D., Szczerbakowa A., Wielgat B.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr 1
7

Cytogenetic analysis of interspecific somatic hybrids between Solanum tuberosum and Solanum nigrum

100%
Boltowicz D., Szczerbakowa A., Wielgat B.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
8

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

100%
Maciejewska U., Skierski J. S., Szczerbakowa A.
Acta Physiologiae Plantarum
|
1999
|
tom 21
|
nr 1
The DNA relative content in nuclei from several Solanum species, which were used as partners for somatic hybridization, were determined using a flow cytometry method. The nuclei were isolated mechanically or via protoplasts from leaves of in vitro grown plants. In the case of S. nigrum as well as S. tuberosum cv. Bzura and dihaploid clone H8105, the nuclei were also obtained from suspension cultured cells by lysis of protoplasts. The source and the method of nuclei isolation affected the pattern of nuclear DNA in the genotypes studied. The mesophyll nuclei showed two distinct peaks on the DNA histograms, whereas the DNA peaks produced by cell suspension nuclei were broad and less distinct. The DNA content in the nuclei, calculated from the DNA histograms of the samples and a DNA standard historgam (Trout Red Blood Cells, having DNA content of 5.05 pg per nucleus), were much lower in mesophyll nuclei than in those obtained from the cell suspension for the same genotypes. The results are discussed in respect of the genetic instability of Solanum genotypes. The usefulness of a flow cytometry approach in somatic hybridization research is also discussed.
9

Somatic hybridization between Solanum nigrum and Solanum tuberosum

100%
Szczerbakowa A., Maciejewska U., Borkowska M., Wielgat B.
Biological Bulletin of Poznań. Supplement
|
1997
|
tom 34
10

Somatic hybridization between the diploids of S. 3 michoacanum and S. tuberosum

88%
Szczerbakowa A., Tarwacka J., Oskiera M., Jakuczun H., Wielgat B.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 5
Interspecific somatic hybrids between a diploid potato clone DG 81-68 susceptible to Phytophthora infestans (Mont.) de Bary and a resistant diploid tuber-bearing species Solanum × michoacanum were generated and analyzed. About 30 regenerants displaying an intermediate morphology were obtained as a result of three separate PEG-mediated fusion experiments. The RAPD analysis confirmed the hybridity of all the regenerants. About 50% of the hybrid plants exhibited vigorous growth and were stable in culture, while the rest of them rooted poorly and grew slowly in vitro. Most of the hybrid clones were at the tetraploid level (70%), while 30% of the clones examined were at the hexaploid level. The S. × michoacanum (+) DG 81-68 hybrids with growth anomalies were aneuploid. The variation in late blight resistance of the hybrid clones was found in detached leaflet tests, with enhanced resistance characteristic for three tetraploid hybrids.
11

Characteristics of the interspecific somatic hybrids Solanum pinnatisectum [plus] S. tuberosum H-8105

88%
Szczerbakowa A., Boltowicz D., Lebecka R., Radomski P., Wielgat B.
Acta Physiologiae Plantarum
|
2005
|
tom 27
|
nr 3A
The morphological, cyto logical and molecular analyses of the plants regenerated after PEG-induced fusion between mesophyll protoplasts from the dihaploid potato clone H-8105 and the wild tuberous disease-resistant species S.pinnatisectum, were performed. A single fusion experiment yielded 313 calli, although only two calli produced shoots. From the rooted shoots, two stable clones (PT-01-1 and PT-01-2) exhibiting different vigor and habitat, were developed. The plants of PT-01-1 clone grew slowly in vitro, produced tubers after transfer to soil but did not set flowers. In contrast, the plants of the vigorous clone PT-01-2 produced both tubers and flowers after transfer to soil. The flower and tuber morphology of PT-01-1 and PT-01-2 regenerants was intermediate in comparison to the parental species. Cyto logical analysis revealed that the PT-01-1 clone was pentaploid and the PT-01-2 clone was tetraploid. The molecular (RAPD) analysis confirmed hybridity of both clones. The preliminary tests on late blight resistance of the hybrids showed no differences with a potato parent.
12

Electrofusion of protoplasts from Solanum tuberosum, S.nigrum and S.bulbocastanum

88%
Szczerbakowa A., Maciejewska U., Pawlowski P., Skierski J. S., Wielgat B.
Acta Physiologiae Plantarum
|
2001
|
tom 23
|
nr 2
13

Organelle DNA analyses in interspecific somatic hybrids of potato

76%
Antonova O., Thieme R., Szczerbakowa A., Fedorina J., Wielgat B., Gavrilenko T.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
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