The purpose of the studies was to establish the current viral agents in etiology and pathogenesis of diarrhoea diseases in dogs maintained as pets in a metropolitan zone. Close laboratory examinations were carried out on 100 alive or dead dogs with more or less intensive diarrhoea. 60 dogs were examined in 1995 and 40 dogs in the next year. 20 healthy dogs (10 in each season) were used as a control group. The feaces and blood samples were collected from live dogs as well as fragments of digestive tracts derived from dead animals were used as the material for the studies. The examinations for parvoviruses were performed using the ELISA test (Canine Parvovirus Test-On Site Biotech, Uppsala). Slidex-Rota-Kit (Bio Merieux, Paris) was applied for diagnosis of rotavirus infections. Direct immunofluorescence technique was used to demonstrate CPV, CCV, CAV and CDV antigens. The serological examinations for detection of specific antibodies anti-CDV, anti-CCV and anti-CAV were performed in 15 dogs using indirect immunofluorescency. The specific antibodies anti-CPV were found in 20% of the examined dogs (titres from 1:160 to 1:640), anti-CAV in 46% (1:40-1:1280) and anti-CCV in 40% (1:20-1:640), respectively. In living dogs examined by means of direct methods, 25% positive results for CPV infection were noted in the first season and 32% in the next one, as well as 6% and 5% for rotaviruses, respectively. In 54 of the cases analised in the 1995 CPV antigen was demonstrated in postmortem samples of digestive tracts. In the same season CAV was found in 18% and CCV - in 3.5% of the samples of the small intestine as well as CDV in 7% of the cases in the stomach. In the 1996 season, positive results for CPV, CAV, CCV and CDV were observed respectively in 36%, 9,0%, 18-55% and 9-18%.
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