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Pasture-feeding of Charolais steers influences skeletal muscle metabolism and gene expression

100%

Cassar-Malek I., Jurie C., Bernard C., Barnola I., Micol D., Hocquette J.-F.

Journal of Physiology and Pharmacology. Supplement

2009

tom 60

nr 3

83-90

A cDNA macroarray resource for gene expression profiling in ruminant tissues involved in reproduction and production (milk and beef) traits

68%

Bernard C., Degrelle S., Ollier S., Campion E., Cassar-Malek I., Charpigny G., Dhorne-Pollet S., Hue I., Hocquette J.-F., Le Provost F., Leroux C., Piumi F., Rolland G., Uzbekova S., Zalachas E., Martin P.

Journal of Physiology and Pharmacology. Supplement

2005

tom 56

nr 3

215-224

cDNA arrays have proven to be useful tools to screen gene expression in many animal species including livestock species. A collaborative program was launched to construct a ruminant cDNA collection, representative of three tissues: Muscle, Embryo and Mammary gland, named MEM. This collection gathers clones mainly arising from 3 non-normalised cDNA libraries: a directed bovine muscle library, a 14-day-old bovine embryo library and a goat lactating mammary library. It is made up of 1896 clones (637 muscle, 882 embryo and 377 mammary cDNAs), selected after sequencing and bioinformatic analyses. Amplification products yielded from these clones as well as controls were printed onto Nylon membranes to generate macroarrays. Hybridisation with relevant cDNA targets allowed checking the location of about 50 cDNAs and the specificity of each sub-set of the repertoire. Macroarrays were hybridised with radiolabelled cDNA complex targets from five different tissues (muscle, embryo, mammary gland, adipose tissue and oocyte). Both somatic and germinal complex targets gave valid hybridisation signals with 45 to 80% of the printed probes. This specific cDNA collection now provides a powerful tool for transcriptomic studies with the ultimate objective to better understand physiological and metabolic functions in ruminants. It will be subsequently included into a forthcoming larger collection.

Coal Tits Periparus ater build larger nests than Blue Tits Cyanistes caeruleus and Great Tits Parus major living in the same Mediterranean coniferous woodland habitat

68%

Lambrechts M. M., Haurez J., Bodineau G., Gagliardi G., Maistre M., Perret P., Pihan P., Wilhelm B., Wilhelm J., Bernard C., Blondel J.

Acta Ornithologica

2016

tom 51

nr 1

The size and shape of the nest are species-specific characteristics that are often associated with environmental factors at the time of breeding. Nests are expected to be larger or thicker in colder environments, although the relationships between nest design and weather differ between species. Here we present the results of an analysis of the external height of the nest wall in Paridae that accepted small standardized nesting boxes for breeding. The study populations were monitored in a relatively cold Mediterranean study area. We found that Coal Tits Periparus ater built higher external nest walls than Great Tits Parus major or Blue Tits Cyanistes caeruleus, after controlling for the first-egg date and clutch size which are assumed to reflect aspects of the quality of the nest builders. Our measures of nest size were not closely associated with the average ambient temperature, but nest walls tended to be shallower when there was more rain. Nest-shape asymmetry, as reflected in the difference in the external height of the nest measured closest to and farthest from the nest-chamber entrance, was observed in all three species, but the average asymmetry was highest in Coal Tits. In asymmetric nests, more nest material was added to the side that was closest to the front wall considered to be the coldest and least protected against harsh weather. Thus, nest size characteristics differ between three ecologically similar species inhabiting the same cavity type in the same coniferous woodland habitat, which would imply that different species do not respond in the same way to the same set of environmental factors.

Ograniczanie wyników

2 Journal of Physiology and Pharmacology. Supplement

1 Acta Ornithologica

3 Bernard C.

2 Cassar-Malek I.

2 Hocquette J.-F.

1 Barnola I.

1 Blondel J.

1 Bodineau G.

1 Campion E.

1 Charpigny G.

1 Degrelle S.

1 Dhorne-Pollet S.

1 Gagliardi G.

1 Haurez J.

1 Hue I.

1 Jurie C.

1 Lambrechts M. M.

1 Le Provost F.

1 Leroux C.

1 Maistre M.

1 Martin P.

1 Micol D.

1 Ollier S.

1 Perret P.

1 Pihan P.

1 Piumi F.

1 Rolland G.

1 Uzbekova S.

1 Wilhelm B.

1 Wilhelm J.

1 Zalachas E.

1 2016

1 2009

1 2005

Wyniki wyszukiwania

Pasture-feeding of Charolais steers influences skeletal muscle metabolism and gene expression

A cDNA macroarray resource for gene expression profiling in ruminant tissues involved in reproduction and production (milk and beef) traits

Coal Tits Periparus ater build larger nests than Blue Tits Cyanistes caeruleus and Great Tits Parus major living in the same Mediterranean coniferous woodland habitat