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Evaluation of the potential yield and primary symptoms of Xanthomonas campestris pv. campestris infection in Asian vegetables grown in the Czech Republic

100%

Kopta T., Penazova E., Jurica M., Pokluda R.

Acta Agrobotanica

2018

tom 71

nr 4

Selected cultivars of Asian brassicacean vegetables were evaluated for their yield potential and susceptibility to bacterial infection. Chinese broccoli, two cultivars of Chinese cabbage (‘Dwarf milk cabbage’ and improved ‘Tahtsai’), and mizuna were grown in the conditions of the Czech Republic. Morphological and yield parameters for the field conditions (plant height, plant diameter, and marketable weight) were verified. In addition, genotypes were tested for resistance to black rot [Xanthomonas campestris pv. campestris (Xcc)] at the planting stage. The results show that a very promising genotype of mizuna, which yielded best (1,478 g per plant). Chinese cabbage (cultivar ‘Dwarf milk cabbage’) also showed high yield values (2,839 g per plant), especially when compared to the reference value for Napa cabbage. Chinese Cabbage 1 (‘Dwarf milk cabbage’) and mizuna also showed a low susceptibility to infection by bacterial black rot at the planting stage. Both cultivars reached Level 2 (median) of infection, which corresponds to the extent of the symptoms on 25% of the leaf surface.

Evaluation of different methods of DNA extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves

88%

Penazova E., Eichmeier A., Cechova J., Baranek M., Pokluda R.

Acta Scientiarum Polonorum. Hortorum Cultus

2015

tom 14

nr 6

The bacterium Xanthomonas campestris pathovar campestris (Xcc) as the causal agent of black rot of cruciferous plants causes considerable losses in agricultural yield all over the world. The control of black rot is difficult as well as the determination of Xccon the basis of morphological parameters or by pathogenicity testing. Ten different possibilities for extraction bacterial DNA followed by PCR detection method were tested to optimize PCR protocol. On the basis of ISTA validated method, three sets of primers UBP 1052F-BACR, DLH 120-125 and ZUP 2309-2310 were used. The results of measured concentration and quality of DNA and efficacy for PCR amplification were compared. Finally, three approaches for DNA extraction within Xanthomonas campestris pv. campestris detection protocol were recommended – commercial kits used for isolation from tissues by Macherey-Nagel and MO BIO and kit intended for microbial cultures by MO BIO.

Ograniczanie wyników

1 Acta Agrobotanica

1 Acta Scientiarum Polonorum. Hortorum Cultus

2 Penazova E.

2 Pokluda R.

1 Baranek M.

1 Cechova J.

1 Eichmeier A.

1 Jurica M.

1 Kopta T.

1 2018

1 2015

Wyniki wyszukiwania

Evaluation of the potential yield and primary symptoms of Xanthomonas campestris pv. campestris infection in Asian vegetables grown in the Czech Republic

Evaluation of different methods of DNA extraction for detection of bacterium Xanthomonas campestris pv. Campestris in cabbage leaves