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The objective of this study was to investigate the effects of dietary n-6:n-3 polyunsaturated fatty acid (PUFA) ratios on the oxidative stress of the liver in goslings. A total of 160 healthy Yangzhou geese was randomly divided into 4 groups, and each group was fed on diets with different n-6:n-3 PUFA ratios: A (12:1), B (9:1), C (6:1), and D (3:1), respectively. The liver was separated and antioxidant functions were evaluated on days 42, 56, and 70. The results showed that the activities of total superoxide dismutase, glutathione peroxidase, and catalase decreased when the geese aged, and were highest on d 42 in each group (P<0.05). The activities of these enzymes increased as the n-6:n-3 PUFA ratios decreased and were highest in the 3:1 group at all tested ages (P<0.05). It was also observed that the levels of H2 O2, malondialdehyde (MDA), and nitric oxide (NO) in liver tissue tended to increase with age, and were relatively higher on d 70. Furthermore, the levels of H2 O2, MDA, and NO decreased as n-6:n-3 PUFA ratios decreased and were lowest at 3:1 and 6:1 at all studied ages (P<0.05). Overall, diets containing comparatively low n-6:n-3 PUFA ratios, such as 3:1 or 6:1, could increase antioxidant function and decrease hepatocyte damage in liver tissue.
This study was conducted to investigate the effects of n-6:n-3 polyunsaturated fatty acid (PUFA) ratios on serum protein and immunoglobulins in growing geese. One hundred and sixty healthy Yangzhou geese were randomly divided into 4 groups: diets were designed as 12:1, 9:1, 6:1, and 3:1, respectively, by varying dietary n-6:n-3 PUFA ratios. Protein and immunoglobulin levels were measured on d 42, d 56, and d 70. The experimental results showed that total protein was highest in group 6:1 on d 42 (P<0.05), albumin, in group 12:1 on d 56 (P<0.05), and globulin, in group 6:1 on d 42 (P<0.05). No significant difference was detected in the albumin/globulin ratio (A/G ratio) among groups (P>0.05); however, the numerical value of the A/G ratio of group 3:1 was higher than in any of the other groups at all sampling ages. On d 56 and 70, IgA and IgM were highest in group 3:1, whereas IgG was highest in group 6:1, and these values were significantly higher than in the other groups (P<0.05). Overall, diets containing low n-6:n-3 PUFA ratios could increase the serum A/G ratio, IgA, IgG, and IgM, in the Yangzhou goose on days 56 and 70.
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