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1

Przeglad metod wykorzystywanych do wykrywania i charakterystyki szczepow Pasteurella multocida i Bordetella bronchiseptica

100%
Markowska-Daniel I., Stepniewska K.
Medycyna Weterynaryjna
|
2009
|
tom 65
|
nr 03
166-170
Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bbr) are important pathogens of humans and many species of animals. At present, several classic and modern techniques are applied for their detection and identification. The review presents an overview of the diagnostic methods that are the most widely used in laboratories, with special emphasis placed on highlighting their advantages and limitations. The choice of the technique depends on the aim of the study. The most often used are classical methods such as microbiological, serological, biochemical, and - in the case of atrophic rhinitis - likewise the morphometric analysis of the cross-section of a turbinated bone. The above-mentioned techniques are not accurate enough because most of the test results must be confirmed by more precise and sensitive techniques. The development of a wide spectrum of molecular techniques has facilitated the undoubted identification of Pm and Bbr. The widest used is PCR basing on the amplification of the gene encoding dermonecrotoxin of Pm and Bbr. Other molecular techniques could be useful for describing the connection among individual components of bacterial cells and their ability to develop the disease. Moreover they could also be used to describe the degree of phylogenetical relationship among field strains. Such information could be used to make a prognosis regarding the appearance of diseases on a particular area. Taking into account the significance of the rapid and precise detection of the pathological agents for immediate and accurate therapy, the continuation of studies aimed at the developing of rapid and sensitive diagnostic techniques is fully recommended and justified.
2

Occurrence of genes encoding virulence factors in Bordetella bronchiseptica strains isolated from infected and healthy pigs

100%
Stepniewska K., Markowska-Daniel I.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The objective of the study was to determine genotypie profiles of Bordetella bronchiseptica (Bbr) strains, based on the occurrence of genes encoding virulence factors, such as flagella (flα), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR. 209 tested Bbr strains were obtained from Polish swine herds with different health status (with progressive atrophic rhinitis - PAR, suspected for PAR, and unknown). In total, seven different Bbr genotypes were determined. In 39.2% of Bbr isolates all three genes were present. In 41.1% of the isolates only two genes were detected. The most common genotype dnt+bfrZ-flα+ was present in 60 (28.5%) Bbr strains, 65% of them were obtained from farms with PAR. Twenty five (12%) Bbr isolates were identified as dnt-bfrZ+flα+ genotype and, as above, they were more frequently isolated from clinical cases of disease (84%). Among 31 (14.8%) strains only flα gene was evident, and in nine (4.3%) only dní gene was present. There were no Bbr strains with bfrZ gene only. These results confirm the heterogenicity among Bbr strains.
3

The Eurasian Reed Warbler (Acrocephalus scirpaceus) breeding in Egypt - a new evidence?

100%
Stepniewska K., Ozarowska A.
The Ring
|
2012
|
tom 34
4

Phenotypic and genotypic characterisation of Pasteurella multocida strains isolated from pigs in Poland

100%
Stepniewska K., Markowska-Daniel I.
Bulletin of the Veterinary Institute in Puławy
|
2013
|
tom 57
|
nr 1
A total of 319 Pasteurella multocida (Pm) strains isolated from pigs in Poland were examined. Phenotypic characterisation included: biochemical tests (to determine species, subspecies, and biovar), capsular typing, and antimicrobial susceptibility. Genotypie characterisation included detection of the toxA gene by PCR. All tested Pm strains were classified as Pm subsp. multocida: 87.2% biovar 3, 10.7%-2 and 0.9%-12. One strain was classified as biovar 1. Three strains of Pm did not suit any of the biovars. Using capsular ty ping methods, 77% of Pm strains isolated from nasal swabs belonged to type D and 33% to type A. Among Pm strains isolated from internal organs, 59.5% belonged to ty pe A and 40.5% to type D. All the isolates showed a high susceptibility to ß-lactams: ampicillin and amoxicilin with clavulonic acid (97.8%), penicillin (86.7%), doxicilline (100%), oxytetracycline (97.8%), and tetracycline (93.2%). It was found that all strains were susceptible to norfloxacin, 97.8% to enrofloxacin, and 95.6% to SxT. 24.4% and 15.6% of the strains were resistant to linco-spectin and tiamulin, respectively. The presence of toxA gene was confirmed by PCR in 20.8% of the strains isolated from nasal swabs and 29.1% of isolates from internal organs.
5

Evaluation of PCR test for detection of dermonecrotoxin of Bordetella bronchiseptica

100%
Stepniewska K., Markowska-Daniel I.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The aim of the study was the evaluation and optimisation of PCR test for the detection of dermonecrotoxin gene (DNT) of Bordetella bronchiseptica. For the optimisation of the test, vaccine strain B16 was used. The optimisation procedure included: estimation of optimal Mg²⁺ concentration, annealing temperature, numbers of cycles, as well as sensitivity. The specificity of PCR test was checked with DNA of other pathogens existing in pigs' respiratory tract. The elaborated test was specific and sensitive to detect DNT gene of B. bronchiseptica, in both clinical samples, as well as in pure culture of the bacteria.
6

Występowanie mieszanych zakażeń układu oddechowego w krajowych stadach świń

81%
Markowska-Daniel I., Kwit K., Stepniewska K., Czyzewska E.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2013
|
tom 08
|
nr 1
7
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Antibiotic susceptibility of bacteria isolated from respiratory tract of pigs in Poland between 2004 and 2008

81%
Markowska-Daniel I., Urbaniak K., Stepniewska K., Pejsak Z.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
29-36
Antibiotic susceptibility of bacteria isolated from nasal swabs and lungs of pigs, to 16 commonly used antibiotics, was determined by disc diffusion test, ß-lactams showed the best activity against Streptococcus suis (S. suis) (> 99% of susceptible strains). The lowest sensitivity of S. suis was evidenced to: tylosin, tetracycline and neomycin (50%, 40% and 25%, respectively). Isolates of Escherichia coli (E. coli) demonstrated the highest susceptibility to cephalosporin (85% strains), gentamicin and norfloxacin (over 74%). The lowest susceptibility of E. coli was demonstrated to tiamulin and penicillin (11.3% and 1.9%, respectively). Over 80% of Actinobacillus pleuropneumoniae (App) strains were susceptible to all antibiotics tested. The highest resistance of App, but demonstrated by below 20% of tested isolates only, was evidenced to neomycin and LxS. Isolates of Pasteurella multocida (Pm), Haemophilus parasuis (Hps) and Arcanobacterium pyogenes (A. pyogenes) were highly susceptible to the most antibiotics included in the analysis. The comparison of the in vitro susceptibility of pathogens to the chemotherapeutics used on Polish farms for the therapy of bacterial infection of pigs within the last five years and the last 10 years, showed an increasing percent of E. coli and S. suis strains resistant to commonly used antibiotics. It is also shown that Pm, Hps, App and A. pyogenes isolates were continuously susceptible to the most chemotherapeutics applied.
8

Progresywne zakaźne zanikowe zapalenie nosa świń - rozpoznawanie, profilaktyka i leczenie

81%
Markowska-Daniel I., Stepniewska K., Samorek M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2008
|
tom 03
|
nr 2
9

zzzn u świń - zapomniana choroba?

81%
Pejsak Z., Jablonski A., Stepniewska K.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2008
|
tom 03
|
nr 4
10

Migration dynamics and directional preferences of passerine migrants in Azraq (E Jordan) in spring 2008

81%
Stepniewska K., El-Hallah A., Busse P.
The Ring
|
2011
|
tom 33
|
nr 1-2
11

Wykrywanie Pasteurella multocida i Bordetella bronchiseptica - czynnikow etiologicznych zakaznego zanikowego zapalenia nosa swin przy pomocy testow PCR

81%
Markowska-Daniel I., Stepniewska K., Pejsak Z.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 01
45-50
Dermonecrotoxigenic (DNT) strains of Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bbr), etiological agents of atrophic rhinitis (AR) of swine, are usually detected by conventional microbiological methods. Currently the main tasks in the diagnosis of infectious diseases is to detect the specific genes of pathogens directly from clinical samples within the shortest possible period of time by using sufficiently specific and sensitive methods. In this study two PCR tests were developed for the detection of genes encoding DNT in Pm and Bbr directly in nasal swabs. They were used for examining 481 nasal swabs collected from pigs raised in 56 farms, suspected of AR or displaying clinical symptoms of PAR. Additionally, the usefulness of PCR in the routine diagnosis of AR was compared with standard microbiological and serological methods. DNT+ Pm were directly evidenced in 74 samples (15.4%) from 27 tested herds (48.2%) while DNT+ Bbr were detected in 196 swabs (40.7%) from 41 farms (73.2%). Using the standard microbiological examination, the presence of bacterial cultures of the morphology typical of Pm was observed in 114 tested samples (23.7%) and those typical of Bbr, in 156 samples (32.45%). In the PCR test performed to confirm the presence of the DNT- -encoding gene in Pm isolates, positive results were obtained in 26 samples (22.8%), while among isolated Bbr strains, 95 out of 156 (60.1%) possessed the gene encoding DNT. In the ELISA test, 22 out of 26 isolates (84.6%) were DNT+. In the case of DNT- isolates of PM, the results of ELISA were in full agreement with PCR results. In total, PAR was detected in 27 farms (48.2%), NPAR in 21 farms (37.5%), and 8 farms (14.8%) were diagnosed as free from AR since no positive results were obtained by either method. Summarizing, the results of serological, bacteriological and molecular examinations clearly demonstrated the usefulness of PCR in the routine diagnosis of AR directly from clinical material.
12
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Phenotypic and genotypic characterization of Bordetella bronchiseptica strains isolated from pigs in Poland

81%
Stepniewska K., Urbaniak K., Markowska-Daniel I.
Polish Journal of Veterinary Sciences
|
2014
|
tom 17
|
nr 1
A total of 209 Bordetella bronchiseptica (Bbr) strains isolated from pigs were examined. Phenotypic study included: biochemical characterization (motility, catalase, oxidase, urease activity, nitrate reduction and growth on MacConkey agar) and antimicrobial susceptibility (disc diffusion method). Genotypic studies based on detection of three genes encoded virulence factors, such as: flagella (fla), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR. Most of the Bbr strains tested had a homogeneous biochemical profile. 97.6% of them provided suitable results in biochemical tests. All Bbr isolates tested showed high resistance to penicillin (100%), linco-spectin (100%) and ceftiofur (97.9%). Over 57% and 43% of Bbr strains were resistant to ampicillin and amoxicillin, respectively. All Bbr isolates showed high sensitivity to most chemotherapeutics used such as enrofloxacin (97.9%), tetracycline (97.9%), oxytetracycline (97.9%), amoxicillin with clavulonic acid (95.8%), florfenicol (90.4%), and gentamicine (77.6%). Over of 94% of Bbr strains were moderately susceptible to norfloxacine. Molecular analysis confirmed that almost all evaluated Bbr strains (94.7%) possessed the fla gene. A lower percentage of isolates had the dnt gene (72.7%) and the lowest percentage of strains (51.7%), had the bfrZ gene.
13

Analiza lekowrażliwości krajowych szczepów bakteryjnych wyizolowanych z układu oddechowego świń w latach 2004–2008

81%
Urbaniak K., Stepniewska K., Markowska-Daniel I.
Pamiętnik Puławski
|
2009
|
tom 151
|
nr 1
14

Dynamics of autumn migration of the Acrocephalus warblers through the Polish Baltic coast

81%
Kozlowska A., Stepniewska K., Stepniewski K., Busse P.
The Ring
|
2009
|
tom 31
|
nr 2
15

Współczesne poglądy na temat epidemiologii progresywnego i nieprogresywnego zakaźnego zanikowego zapalenia nosa świń

81%
Markowska-Daniel I., Samorek M., Stepniewska K.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2008
|
tom 03
|
nr 1
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Liczebność ptaków wodnych na Zatoce Gdańskiej w okresie maj 2019–kwiecień 2020

81%
Meissner W., Stepniewska K., Bzoma S., Kosmicki A.
Ornis Polonica
|
2020
|
tom 61
|
nr 3
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Liczebność ptaków wodnych na Zatoce Gdańskiej w okresie wrzesień 2017 - kwiecień 2018

71%
Meissner W., Stepniewska K., Kosmicki A., Kozakiewicz M., Sciborski M.
Ornis Polonica
|
2018
|
tom 59
|
nr 2
18

Opracowanie testu PCR do wykrywania genu kodujacego dermonekrotoksyne Pasteurella multocida w wymazach z nosa swin

71%
Markowska-Daniel I., Jablonski A., Nowak A., Stepniewska K., Pejsak Z.
Medycyna Weterynaryjna
|
2009
|
tom 65
|
nr 08
542-545
Atrophic rhinitis is a disease of high economic impact for pig production, caused by Pasteurella multocida (P.m.) strains able to produce dermonecrotoxin (DNT). In general the diagnosis of pigs’ infection with P.m. is based on the direct detection of bacteria from nasal swabs and their identification as well as on the detection of DNT specific antibodies by using an ELISA test. The aim of the study was the elaboration of a PCR test for the detection of dermonecrotic strains of P.m. directly in nasal swabs. The optimalization of the process includes: estimation of Mg²⁺ concentration, the temperature of primer hybridization and the number of cycles. The specificity and sensitivity of the test was estimated for both bacterial culture and nasal swabs. The optimal conditions were as follow: Mg²⁺ concentration - 2.5 m M/reaction; the temperature of primers hybridization - 56°C; and the number of cycles - 50. The sensitivity of the test for both the culture and nasal swabs was 2.5 × 10³ cfu/ml. The test was also highly specific - the product of 501 bp was detected only in the samples containing DNA of P.m. Summarizing, the elaborated test was specific and sensitive enough to be used for routine detection of P.m. DNT strains in clinical samples and for the elimination of the carriers from the herd.
19

Lekowrażliwość wybranych krajowych szczepów bakteryjnych wyizolowanych od chorych i padłych świń w latach 2004 - 2010

61%
Zmudzki J., Grzesiak A., Nowak A., Urbaniak K., Stepniewska K., Markowska-Daniel I., Pejsak Z.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2012
|
tom 07
|
nr 1
20
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Liczebność ptaków wodnych na Zatoce Gdańskiej w okresie wrzesień 2018–kwiecień 2019

61%
Meissner W., Stepniewska K., Kaszak S., Kosmicki A., Janczyszyn A., Kozakiewicz M.
Ornis Polonica
|
2019
|
tom 60
|
nr 3
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