Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 2

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Transplantation of stem cells is currently investigated as an option for treatment of stroke. In this study we transplanted adult human cells NNC1 derived from brain biopsy after experimental stroke in rats. Transient ischemia in rats was performed by the fi lament model of middle cerebral artery occlusion (fMCAO). About 3×105 NNC1 cells were transplanted near to the necrotic area 7 days after fMCAO and the brain of treated rats was investigated 4 weeks after transplantation. Rats were immunosuppressed by daily injections of cyclosporine A, prednisolone and azathioprine. Grafted cells were localized by anti-human specifi c antibodies HuNu and MTC02. Cells appeared to be located dispersed, however there was almost no cell-migration. The number of localized NNC1 cells 4 weeks after transplantation was rather limited indicating a reduced survival rate which was probably caused by host versus graft rejection processes. The latter is refl ected by activation of Ox42 and ED1 positive microglia/macrophages. Also, we detected CD8 and TCR positive T-cells infi ltrating to the necrotic area. Immunocytochemical analysis revealed expression of nestin and βIII-tubulin by grafted NNC1. Interestingly, some βIII tubulin expressing NNC1 were found to be located in blood vessels. In conclusion, both the survival and the capacity to neuronal differentiation of grafted NNC1 cells are limited.
The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.