Wyniki wyszukiwania

1

Bialka klasy PR10 lubinu zoltego

100%

Sikorski M. M.

Działalność Naukowa PAN. Wybrane Zagadnienia

|

2003

|

tom 15

76-79

2

Structure and expression of yellow lupin genes coding for LIR18A and LIR18B proteins of Betvl family

100%

Sikorski M. M.

Biological Bulletin of Poznań. Supplement

|

1995

|

tom 32

3

Expression of Lupinus luteus cDNA coding for PR10 protein in Escherichia coli: Purification of the recombinant protein for structural and functional studies

100%

Sikorski M. M.

Acta Biochimica Polonica

|

1997

|

tom 44

|

nr 3

The cDNA clones coding for two pathogenesis-related protein homologues of PR10 class, LlPR10.lA and LlPR10.1B, were identified in yellow lupin expression library of uninfected roots. The contribution of PR10 proteins to the overall mechanism of plant defence still remains unknown. In order to elucidate the structure and function of lupin PR10.1A protein, a substantial quantity of the protein was produced in an E. coli expression system using plasmids of pET-se- ries: pET-3a and pET-15b, carrying the T7 promoter. Both plasmids with sub- cloned LlprlO.la gene were overexpressed in E. coli, strain BL21(DE3)pLysS. The recombinant L/PR10.1A protein, overproduced in bacterial cells transformed with the pET-3a/LlprlO.la plasmid, was purified to homogeneity from the insoluble "inclusion bodies" by ammonium sulphate fractionation and two sequential chromatographic steps: ion-exchange chromatography on DE 52 cellulose followed by size exclusion chromatography on Superdex 75 FPLC column. The (His)e LlPR10.lA protein overproducted in E. coli cells harbouring the pET-15b/LlprlO.la plasmid was purified by chromatography on Ni2+- charged His.Bind Resin. Western blot analysis with rabbit serum containing anti-£/PR10.1AN antibody revealed identical immunochemical properties of the two recombinant polypeptides and native LIPR10.1A protein. The recombinant protein produced in pET-3a plasmid was renatured from its insoluble form, concentrated up to 22 mg/ml and submitted to crystallisation. However, the LIPR10.1A protein expressed in pET-15b plasmid precipitated from the solution when at a higher concentration (10 mg/ml). This preparation was used at a lower concentration as an antigen for the preparation of polyclonal antibodies for immunochemical studies.

4

Struktura i organospecyficzna ekspresja genow kodujacych roslinne bialka klasy PR10

63%

Sikorski M. M., Biesiadka J.

Biotechnologia

|

1999

|

nr 3

13-35

5

Expression of Lupinus luteus nodulin-45 gene in E.coli cells. Mutagenesis of coding sequence by PCR method

63%

Sikorski M. M., Rozek M.

Acta Biochimica Polonica

|

1994

|

tom 41

|

nr 2

6

Identification by affinity chromatography of wheat germ ribosomal proteins which interact with RNA

51%

Przybyl D., Sikorski M. M., Legocki A. B.

Acta Biochimica Polonica

|

1981

|

tom 28

|

nr 2

7

DNA microarray application for gebe expression profiling in plants

51%

Wloszczak W., Handschuh L., Figlerowicz M., Sikorski M. M.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr Supplement 1

8

RNAi-mediated gene silencing in legumes

51%

Handschuh L., Florek B., Sikorski M. M., Figlerowicz M.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

9

Structural and functional characteristics of two novel members of pathogenesis-related multigene family of class from yellow lupine

45%

Handschuh L., Femiak I., Kasperska A., Figlerowicz M., Sikorski M. M.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 4

783-796

PR-10 proteins (pathogensis-related), ubiquitous within the plant kingdom, are usually encoded by multigene families. To date we have identified 10 homologous pr-10 genes in a yellow lupine cDNA library. Here, the structure and expression of two newly identified yellow lupine pr-10 genes (LlYpr10-2b and LlYpr10-2f) are presented. Many potential regulatory sites were found in both gene promoters including common ones as well as those unique for each gene. However, promoter deletion analysis in transgenic tobacco plants revealed similar patterns of reporter gene (gus) expression. Shortened fragments of both gene promoters studied caused high GUS activity in leaves (along vascular bundles), stamen stigma, anthers and pollen grains. When conjugated with longer LlYpr-10.2 promoter fragments, GUS was additionally present in petal edges. Only a long fragment of the LlYpr10-2b gene promoter caused GUS expression in the stem. In yellow lupine the pr-10.2 genes are present in all studied organs, but their level of expression depends on the stage of development and is affected by wounding, oxidative stress and salicylic acid treatment. Silencing of the Llpr-10.2b gene in 4-week-old yellow lupine plants did not lead to any visible symptoms, which suggests that the function of the silenced gene is supplemented by its close homologues, still present in the studied plants.

10

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

New insights into the signaling and function of cytokinins in higher plants

39%

Ciesielska A., Ruszkowski M., Kasperska A., Femiak I., Michalski Z., Sikorski M. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2012

|

tom 93

|

nr 4

11

Coordinated synthesis of leghemoglobin and root protein R18 in yellow lupin

33%

Sikorski M. M., Szybiak-Strozycka U., Strozycki P., Golinska B., Madrzak C. J., Kamp R. M., Wittmann-Liebold B., Legocki A. B.

Acta Biochimica Polonica

|

1989

|

tom 36

|

nr 1

12

Structural and thermodynamic aspects of plant-hormone binding by CSBP

33%

Pasternak O., Bujacz G. D., Yasuyuki F., Hashimoto Y., Sikorski M. M., Jaskolski M., Jelen F., Otlewski J.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr Supplement 1

Ograniczanie wyników

Bialka klasy PR10 lubinu zoltego

Structure and expression of yellow lupin genes coding for LIR18A and LIR18B proteins of Betvl family

Expression of Lupinus luteus cDNA coding for PR10 protein in Escherichia coli: Purification of the recombinant protein for structural and functional studies

Struktura i organospecyficzna ekspresja genow kodujacych roslinne bialka klasy PR10

Expression of Lupinus luteus nodulin-45 gene in E.coli cells. Mutagenesis of coding sequence by PCR method

Identification by affinity chromatography of wheat germ ribosomal proteins which interact with RNA

DNA microarray application for gebe expression profiling in plants

RNAi-mediated gene silencing in legumes

Structural and functional characteristics of two novel members of pathogenesis-related multigene family of class from yellow lupine

New insights into the signaling and function of cytokinins in higher plants

Coordinated synthesis of leghemoglobin and root protein R18 in yellow lupin

Structural and thermodynamic aspects of plant-hormone binding by CSBP