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A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures

100%

Dechaux C., Boitel-Conti M.

Acta Biologica Cracoviensia. Series Botanica

2005

tom 47

nr 1

Hairy root culture plays an important role in investigation of alkaloid production in culture in vitro. The complexity of scientific work and the production capacity limitations associated with whole plant systems and unorganized cells can be overcome using culture of hairy roots. This paper presents an example of a strategy to produce secondary metabolites from plants, in which we investigated the alkaloid pathway, modified gene expression, and cultivated and optimized hairy root growth in a bioreactor. Datura innoxia whole plant was transformed by Agrobacterium rhizogenes, and the obtained hairy roots were studied for their tropane alkaloid production. Optimization of medium led to an increase of scopolamine production in hairy root culture of Datura innoxia by increasing the produced biomass, but genetic engineering seems to be the best way to increase the accumulation of scopolamine. These root cultures were not able to stably overaccumulate scopolamine. The involvement of putative enzymatic regulation of the tropane alkaloid pathway is discussed.

Comparison of growth properties, alkaloid production and water uptake of two selected Datura hairy root lines

61%

Lanoue A., Boitel-Conti M., Dechaux C., Laberche J. C., Christen P., Sangwan-Norreel B.

Acta Biologica Cracoviensia. Series Botanica

2004

tom 46

185-192

Two selected hairy root lines of Datura, D. innoxia and D. candida x D. aurea were compared for their performance in growth and tropane alkaloid productivity in shake flasks. Specific consumption rate and biomass yield on sucrose and nitrates during the phase of maximum growth were calculated versus changes in liquid medium volume during culture. Growth parameters were found to be better in D. innoxia hairy root cultures than in D. candida x D. aurea. Overall productivity of hyoscyamine in D. innoxia (2.1 mg l-1 d-1) was double that in D. candida x D. aurea (1 mg l-1 d-1). However, hybrid transgenic roots produced 0.5 mg l-1 d-1 of scopolamine. The relationship between water uptake and root growth was studied. During the first days of culture, water was released into the culture medium and then reabsorbed in the root tissue. After that, water uptake on a biomass basis in D. innoxia and D. candida x D. aurea transformed root cultures reached 0.74 and 0.53 ml g-1 FW, respectively. The correlation between biomass accumulation and liquid medium volume could be used as a simple and inexpensive method for indirect estimation of root growth.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

2 Boitel-Conti M.

2 Dechaux C.

1 Christen P.

1 Laberche J. C.

1 Lanoue A.

1 Sangwan-Norreel B.

1 2005

1 2004

Wyniki wyszukiwania

A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures

Comparison of growth properties, alkaloid production and water uptake of two selected Datura hairy root lines