Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

CNS remyelination-from mechanism to medicine

100%

Franklin R. J. M.

Acta Neurobiologiae Experimentalis

2015

tom 75

nr Supl.

Remyelination, the process by which new myelin sheaths are restored to demyelinated axons, represents one of the most compelling examples of adult multipotent stem cells contributing to regeneration of the injured CNS. This process can occur with remarkable efficiency in multiple sclerosis (MS), and in experimental models, revealing an impressive ability of the adult CNS to repair itself. However, the inconsistency of remyelination in MS, and the loss of axonal integrity that results from its failure, makes enhancement of remyelination an important therapeutic objective. There is now compelling evidence that ageing is the major contributor to the declining efficiency of remyelination and that this is largely due to a failure of stem cell differentiation. This talk will review recent studies we have undertaken aimed at obtaining a detailed understanding of the mechanisms of regulating differentiation during remyelination and hence identifying novel therapeutic targets.

Dissecting of microenviromental nishes for OPC differentiation

39%

Ulanska J., Zhao C., Pohl H., Suter U., Kaminska B., Franklin R. J. M., Zawadzka M.

Acta Neurobiologiae Experimentalis

2011

tom 71

nr S

New myelinating oligodendrocytes during CNS remyelination are derived from proliferating oligodendrocyte precursor cells (OPC). Using cre/lox technology for OPC fate mapping we have previously demonstrated that these cells give rise not only to oligodendrocytes but also astrocytes and Schwann cells in response to demyelination. We have demonstrated that Schwann cells, derived form OPC, occupied almost exclusively the tissue around blood vessels in astrocyte-deficient areas. It suggests that the peri-vascular microenvironmental niche is the critical determinant of this alternative fate choice phenomenon. The aim of present study was to identify and characterise the microenviromental factors and their downstream cellular effectors determining the fate of adult CNS precursor cells which could be modulated in order to control the remyelination process. We used a well-defined demyelination model that involves bilateral stereotaxic injection of ethidium bromide into the brain white matter of adult rats. At 6 and 10 days after lesion animals were sacrificed and the fresh frozen sections were subjected to immunohistochemical detection of endothelial cell markers, to establish the area of vascular and non-vascular niches. Tissue was then microdissected from pre-defined niches by using infrared and ultraviolet laser capture microdissection system. The microdissected cells represent the in vivo state of the cells at the time of cells recruitment and early differentiation. The tissue was collected as a separate niche pools and RNA was extracted for global gene expression analysis. The major outcome of present work is identification of microenviromental factors and quantitative spatial and temporal profile of their expression in the specific niche. Our data serves as a starting point to explore in details the molecular pathways involved in multipotentiality of precursor cells induced by mixed extrinsic microeviromental signals.

Ograniczanie wyników

2 Acta Neurobiologiae Experimentalis

2 Franklin R. J. M.

1 Kaminska B.

1 Pohl H.

1 Suter U.

1 Ulanska J.

1 Zawadzka M.

1 Zhao C.

1 2015

1 2011

Wyniki wyszukiwania

CNS remyelination-from mechanism to medicine

Dissecting of microenviromental nishes for OPC differentiation