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The investigations were carried out in field experiment conditions on light soil (low content of available magnesium). There were used two forms of magnesium fertilizers: magnesium sulphate and dolomite. Magnesium increased cereals crops and contents of Mg in vegetative and generative parts of tested plants: oat, barley, winter wheat, triticale.
W latach 2005-2007 w gospodarstwie ekologicznym położonym na Pojezierzu Starogardzkim przeprowadzono eksperymenty polowe na glebie mineralnej piaszczystej oraz na glebie murszowatej. Ponieważ wspólną cechą obydwu badanych gleb była niska zasobność w przyswajalny potas, postanowiono określić rolę mineralnego nawożenia potasem na plonowanie owsa. W każdym z lat badań przedplonem dla owsa na glebie murszowatej było pastwisko przemienne, a na glebie mineralnej seradela. Uprawiano oplewioną odmianę owsa Szakal. Glebę nawożono siarczanem potasu w dawce 80 kg K₂O na 1 ha na glebie mineralnej oraz 80 i 120 kg na glebie murszowatej. Określano wielkość plonów ziarna i słomy, a także strukturę plonowania oraz cechy morfometryczne. Okazało się, że pomimo zaliczenia obydwu gleb do kategorii o niskiej zasobności w potas, skala reakcji owsa na nawożenie tym składnikiem była różna. W przypadku gleby mineralnej była na granicy istotności. Plon ziarna na obiektach nawożonych i nienawożonych średnio za 3-letni okres wyniósł odpowiednio 2,54 i 2,76 Mg z ha. W przypadku gleby murszowatej uzyskano wysoce istotną zwyżkę wydajności ziarna. W obiekcie kontrolnym plon ziarna wyniósł zaledwie 0,34 Mg z ha, podczas gdy w wariancie z 80 kg K₂O zebrano 2,64 Mg ziarna z ha, a na 120 kg dawce - 3,78 Mg z ha.
Municipal sewage sludge is an unavoidable byproduct of the contemporary live and business activities of people. Proper handling and utilization of sewage sludge continue to create serious problems in Poland although this waste product is a source of both organic carbon and macronutrients. The present study has been carried out in order to assess the influence of municipal sewage sludge on the content of available forms of Cu, Zn and Mn in soil. A microplot experiment set up according to the random sub-block method was conducted in 2005-2008. The experiment was established on anthropogenic, humic urban soil developed from loamy sand rich in phosphorus and magnesium, but poor in potassium, and alkaline in reaction. The design of the trials comprised 5 doses of municipal sewage sludge from the £yna Municipal Wastewater Treatment Plant in Olsztyn: 0, 70, 140, 210 and 280 Mg ha–1 of fresh matter. The tests have demonstrated that a dose of sewage sludge had a significant effect on the content of available forms of Cu, Zn and Mn in soil. In alkaline soil, however, the observed increase in the content of available forms of Cu, Zn and Mn was not hazardous to the environment, but could improve the plant nutrition with these elements. It is highly probable that the availability of Cu may increase in the second and third year after the application of sewage sludge. The accumulation of soluble Zn in soil started to decrease in the second year, but did not become significantly limited until four years after sewage sludge application. Sewage sludge raised the amount of soluble manganese in soil during the first three years, but in the final year of the experiment the quantity of soluble Mn in soil did not undergo any significant fluctuations.
Corn was grown for green matter in a pot experiment, in which soil was fertilized with composts alone or in combination with 3 g N per pot (urea). The composts were made in wooden boxes, measuring 50×60×60 cm. They were composted for 3.5 months, until the temperature in the composts became stable and equal to the ambient temperature. Rates of the composts were balanced with amounts of added nitroge, such as 6.0 g N per pot. A one-factor experiment was conducted in Kick-Brauckmann pots, kept in a greenhouse at the University of Warmia and Mazury in Olsztyn. After harvest, fresh matter of stems and leaves as well as corn cobs was determined. Next, the plant samples were dried to determine the dry matter content and finally, after mineralisation, the concentration of Cu, Zn and Mn was determined by atomic absorption spectrophotometry. The composts significantly reduced the concentration of zinc and manganese in the vegetative yield of cor (lstems and leaves). Urea applied in combination with the composts very strongly increased the concentration of manganese and, to a lesser degree, the level of zinc and copper in vegetative organs. In corn cobs, the application of urea increased only the content of manganese. Urea had a stronger effect on increasing the weight of cobs rather than the vegetative mass of corn plants. The uptake of Cu, Zn and Mn was more evidently conditioned by the accumulation of these elements in dry matter than by the total weight of corn plants. The extent of the relationship between the uptake of Cu, Zn and Mn and their concentration of corn biomass is expressed the by corresponding correlation coefficients: 0.66, 0.65 and 0.68.
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A large area trial involving four methods of soil fallowing (goat’s rue, traditional fallow, goat’s rue + brome grass, brome grass) was established in the spring 1996. This paper contains the results of our investigations concerning the content of macroelements in plants in the years 2000-2004. The weakest accumulation of nitrogen, potassium and magnesium by mono- and dicotyledonous plants occurred in the traditional fallow field; slightly richer concentration of the macroelements was determined in plants growing on the object sown with brome grass. Goat’s rue, both in a monoculture and mixed with brome grass, stimulated plants to accumulate more nitrogen, phosphorus, potassium and magnesium. Using goat’s rue to raise the content of macroelements in plants can prevent transfer of those elements to lower soil layers.
A response of plants grown in crop rotation to form of magnesium fertilizers (magnesium sulphate or dolomite), dose and frequency of application of dolomite were studied in the field experiment. It was shown, that fertilization of light soil with magnesium increased oat and spring barley yield. Both of tested magnesium fertilizers (magnesium sulphate and dolomite) in the same way influenced on yield level of investigated plants. Also, positive effects of applying magnesium fertilizers were increasing of magnesium, nitrogen and phosphorus in index phase, grain and straw.
The purpose of this study has been to evaluate the effect of foliar application of PAH organic chemical compounds (anthracene, pyrene) on yield, chemical composition and uptake of nutrients by cv. Vilmorin butterhead lettuce (Lactuca sativa L.). Lettuce was grown under minimum and three-fold higher abundance of substrate in nutrients, as determined according to threshold amounts. A pot trial was established in four replicates and performed twice, in the spring of 2008 and 2009, in a greenhouse at the University of Warmia and Mazury in Olsztyn. Lettuce seedlings were planted in pots containing 10 dm3 of mineral substrate. Fertilization (N, P, K, Mg, Na and Cl) was carried out prior to the planting of lettuce. Under the minimum nutrient abundance of the substrate, all the dose of nitrogen was supplied as a pre-sowing treatment, whereas when the abundance in nutrients was raised three-fold, the nitrogen dose was divided (2/3 pre-sowing and 1/3 10 days after planting). Contamination of lettuce plants with anthracene (ANT) or pyrene (PYR) and their mixture started 10 days after planting lettuce. Foliar application of either of the PAHs continued for 25 days until the vegetative growth of lettuce terminated. Determination of the concentration of macronutrients (N, P, K, Mg, Ca and Na) was performed using standard methods on the mineralised (H2SO4+H2O2), previously dried at 60ºC lettuce plant material. The determinations were completed by referring to certified material (CTA-VTL-2). The amount of fresh mass of butterhead lettuce depended primarily on the abundance of the substrate in nutrients. The three-fold increase in the substrate’s abundance in N, P, K, Mg, Na and Cl caused an increment in the yield of lettuce head mass by 13.3%. Foliar application of ANT and PYR caused an increase in the yield of lettuce head mass. The concentration of N, K, Na, Mg, Ca and Mg in lettuce was modified first of all by the abundance of the substrate and, to a lesser degree, by the applied PAHs.
The objective of a study conducted in the years 2001-2003 was to determine the quantitative and qualitative composition of fungal populations colonizing the roots and radical zones – rhizosphere and rhizoplane – of oriental goat’s rue cultivated with and without the application of fertilization. Root samples were collected from particular experimental objects before flowering to isolate fungi according to the methodology described by STRZELCZYK (1968). At the same time the influence of fungi of genus Trichoderma on potentially pathogenic fungi of Botrytis cinerea and genus Fusarium was investigated. The largest fungal colony was found in the rhizosphere, where yeast-like fungi accounted for 80% of all isolates. Potentially pathogenic fungi of genus Fusarium constituted 2% only. They were isolated during all years of the study. Fungi of genus Fusarium were isolated more frequently from the rhizoplane and roots than from the rhizosphere (13.0 and 17.4% respectively). Among the other pathogens there were a few species of: Botrytis cinerea, Sclerotinia sclerotiorum and of genus Ascochyta. Fungi of the order Mucorales were quite common in the rhizoplane (43.3% of all isolates), whereas species of the genera Gliocladium and Trichoderma, antagonistic towards pathogens, accounted for 16.6%. Species belonging to the genera Gliocladium and Trichoderma were isolated more frequently from roots than from rhizoplane (25%), whereas fungi of the order Mucorales were less frequent (15.6%). Mineral fertilization modified the quantitative composition, and to some degree also the qualitative composition, of fungi colonizing the radical zones of oriental goat’s rue. The most isolates were obtained from the combination without fertilization (35.5% of all isolates). Potentially pathogenic fungi were isolated more frequently from the rhizoplane than from the rhizosphere in combination without fertilization and from the roots in the combination without fertilization as well as with fertilization at 80 kg P₂O₅ – and 160 K₂O kg x ha⁻¹. In the test of antagonism the species of genus Trichoderma inhibited growth of the tested pathogens of genus Fusarium and Botrytis cinerea.
Toxic compounds which belong to PAHs are generated during all types of combustion of fuels and other substances as well as a result of natural processes (mineralisation). Products which appear during the above processes eventually reach soil, where they accumulate. The objective of this study has been to evaluate the effect of anthracene and pyrene accumulated in soil on yield, concentration of macronutrients (N, P, K, Mg, Ca, Na) and their uptake by cv. Vilmorin butter lettuce (Lactuca sativa L.) grown under the minimum and 3-fold enriched abundance of substrate. A pot experiment in four replicates was carried out twice in a greenhouse at the University of Warmia and Mazury in Olsztyn, in the spring of 2007 and 2008. Supplementary fertilisation was applied before planting butter lettuce. At the first rate of fertilisation, nitorgen was introduced in a full dose before planting lettuce, but at the triple rate of nutrients in substrate, it was divided into 2/3 of the dose before planting and the remaining 1/3 applied to soil 10 days after planting. Soil contamination with anthracene (ANT) and pyrene (PYR) or their mixture started 10 days after planting lettuce. Soil application of the two PAHs was performed 5 times at five-day intervals until the end of the growing season of lettuce. Determination of macronutrients (N, P, K, Mg, Ca, Na) was accomplished with standard methods after mineralisation (H2SO4 + H2O2) of the plant material dried at 60oC. The determinations were peformed in comparison to certified material (CTA-VTL-2). Increasing the abundance of substrate in nutrients (N, P, K, Mg, Na, Cl) by three-fold led to a 13.7% increase in yield of lettuce heads. The PAH compounds present in the substrate depressed lettuce yield. This tendency was more evident when anthracene rather than pyrene contaminated soil. ANT and PYR depressed the concentration of nitrogen but raised the concentration of calcium in substrate less abundant in nutrients.
The object of the experiment was fodder galega (Galega orientalis Lam.) cultivated in 2001-2003 as field crop on three plots: 1. without fertilization, 2. 40 kg P2O5 × ha-1 and 80 kg K2O × ha-1, 3. 80 kg P2O5-1 × ha and 160 kg K2O × ha-1. During the dry and warm vegetation season of 2002 almost two times fewer isolates were obtained from the leaves than in 2003 that was the most abundant in fungi. Yeasts-like fungi (30% of the total number of isolates) and saprotrophic fungi with dominated species: Acremonium strictum (8.5%), genus Epicoccum (7.8%), Humicola (9.5%) and Penicillium (18.9%) were the fungi most frequently populating the leaves of galega. The share of pathogens in the total number of isolates obtained from the phyllosphere was 10.6%. They were represented by fungi of Ascochyta spp., Botrytis cinerea, genus Fusarium, Phoma medicaginis and Sclerotinia sclerotiorum. Reduction by 1.9 to 4.6% in the number of fungi isolated from the phyllosphere of galega without fertilization as compared to galega cultivated in combinations with fertilization was recorded. Generally, the smallest number of pathogens was recovered from galega fertilized with 40 kg P2O5 × ha-1 and 80 kg K2O × ha-1. B. cinerea most frequently populated galega in combination without fertilization, genus Fusarium fungi in combination without fertilization and with fertilization with 80 kg P2O5-1 × ha and 160 kg K2O × ha-1, while Ascochyta spp. were isolated from galega with fertilization only.
Multi-component fertilizers are increasingly used due to their easy application, solubility and complex composition. A clear advantage of multi-component fertilizers over simple fertilizers is that the former supply a combination of nutrients at a time. The objective of this study was to determine the effect of multi-component fertilizers, Amofosmag 4 and Amofosmag 3, on winter triticale yield, and the content and uptake of macronutrients. A three-year field experiment (2008–2010) was carried out in a randomized block design at the Research and Experimental Station in Tomaszkowo, at the University of Warmia and Mazury in Olsztyn (NE Poland). The experiment comprised three fertilization treatments in four replications: control treatment (simple fertilizers) and two treatments with mixed multi-component fertilizers, Amofosmag 4 and Amofosmag 3. The tested crop was winter triticale cv. Grenado. Wet mineralized plant samples were assayed for the content of: total nitrogen – by the hypochlorite method, phosphorus – by the vanadium-molybdenum method, calcium and potassium – by atomic emission spectrometry (AES), and magnesium – by atomic absorption spectrometry (AAS). In most cases, the application of Amofosmag 4 and Amofosmag 3 increased the yield of winter triticale grain and straw, in comparison with simple fertilizers. The concentrations of the analyzed macronutrients in triticale were similar in all fertilization treatments, thus pointing to a comparable effect of the applied fertilizers, except for the nitrogen content of triticale grain which was highest in plots fertilized with simple fertilizers, compared with the other treatments. Differences in the chemical composition of triticale plants were observed between successive years of the study. The highest total uptake of phosphorus, potassium and magnesium by winter triticale was noted in plots fertilized with Amofosmag 3. Nitrogen uptake was higher in the control treatment, and calcium uptake in the Amofosmag 4 treatment.
The objective of this study was to determine the effect of mixed multi-component fertilizers, Amofosmag 4 and Amofosmag 3, on winter rye yield, and the content and uptake of macronutrients. A three-year field experiment (2008–2010) was carried out in a randomized block design at the Research and Experimental Station in Tomaszkowo, at the University of Warmia and Mazury in Olsztyn. The tested crop was winter rye (Secale Cereale L.) cv. Dańkowskie Diament. The obtained results showed that fertilization multi-component fertilizers, especially Amofosmag 3 has the effect of increasing the yield of grain and straw of winter rye as compared to simple fertilizer. The concentrations of the analyzed macronutrients in winter rye grain and straw varied insignificantly between fertilization treatments. Simple and multi-component fertilizers exerted a comparable effect on the mineral composition of the test crop. Significant differences were observed in this respect between successive years of the study. The highest total uptake of nitrogen, potassium and calcium by rye plants was noted in treatments with simple fertilizers, and somewhat higher phosphorus uptake was observed in the Amofosmag 4 treatment. Total magnesium uptake was similar in all treatments.
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