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The occurrence of black-coated individuals in wolf Canis lupus Linnaeus, 1758 populations is not surprising itself, but their presence in populations recovering from a severe numerical decline has been considered a possible sign of crossbreeding with the domestic dog. In the northern Apennines (Italy), black wolves occur at a non-negligible frequency. In a 3300 km area, 22% of wolves observed and 23% of all dead wolves found were represented by animals with a completely black coat. One 'black' wolf belonging to the studied population was analysed by a set of microsatellite loci, and no trace of hybridization was found in its ancestry. This result induced us to consider the occurrence of a black phenotype in this area possibly derived from a natural combination of wolf alleles in coat colour determining genes, and not necessarily as the result of crossbreeding with the domestic form.
A coarse scale analysis was carried out of factors affecting rendezvous site selection and fidelity to pup raising areas in wolfCanis lupus Linnaeus, 1758 packs inhabiting the north eastern Apennines, Italy. From 1993 to 2004, 44 rendezvous sites were identified, and compared with random sites for variables related to topography, habitat, and human presence. Rendezvous sites were significantly more frequent inside protected areas and at a significantly greater distance from paved roads and villages than random sites. Moreover, they were located on a significantly steeper slope and at a higher absolute elevation than random sites, whereas we did not observe selection of a particular aspect. Deciduous forests were selected, whereas open areas were not used. Canopy cover above 70% was positively selected. Distance from protected areas border and paved roads, and presence of deciduous forest proved the main factors affecting rendezvous site distribution. In four cases we observed the reuse of the same rendezvous sites for two consecutive years. Some packs tended to locate their rendezvous sites in small portions of their territory, thus implying that some areas are more suitable for pup raising.
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