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1

Choroby stawow o podlozu immunologicznym u psow i kotow - cz.I

100%
Madej J. P.
Weterynaria w Praktyce
|
2006
|
tom 03
|
nr 2
84
2

Choroby stawow o podlozu immunologicznym u psow i kotow - cz.II

100%
Madej J. P.
Weterynaria w Praktyce
|
2006
|
tom 03
|
nr 3
6-8
3

Mechanizmy obrony przeciwnowotworowej - przyczyny niepowodzeń

63%
Madej J. A., Madej J. P.
Medycyna Weterynaryjna
|
2015
|
tom 71
|
nr 09
Neoplastic transformation is accompanied, among others, by the inhibition of apoptosis. Four mechanisms that allow cells to avoid apoptosis are well known: loss of APAF1 (apoptosis-inducing factor 1), up-regulation of caspase inactivating apoptosis inhibitors, expression of FasL (Fas ligands) on the cell surface, and production of caspase 8 inhibiting protein (FLIP – FADD – like interleukin – 1β converting proteins). Moreover, the roles of RB1 and TP53 genes in the neoplastic process have been described. Tumor cells can show mutations of genes responsible for the expression of proteins regulating the cell cycle and of proteins controlling apoptosis, e.g. TP53 protein. Neoplastic cells seek to avoid not only apoptosis, but also necrosis, inflammatory cells, and the reaction of the immunological system. The types of neoplastic antigens, i.e. TSA (tumor specific antigens) and TAA (tumor associated antigens), as well as the possibilities of their recognition by the organism burdened with neoplasm, have been described. Furthermore, the mechanisms of weakening the immunological response by neoplastic cells and the role of inflammatory cells and inflammatory response in destroying neoplasms (mainly the cellbased response i.e. cells such as NK, LAK, Tc, Th, and macrophages) have been presented. Macrophages have received special attention because of the role of macrophages associated with tumors (e.g. mammary gland cancer in dogs) in causing an abnormal (non-canonical) course of the Wnt pathway. Paradoxically, the immune response sometimes promotes neoplastic growth by the so-called immune facilitation effect. After the first stage (i.e. the limitation of apoptosis), the necrosis of neoplastic cells and inflammatory infiltration occur, followed by unlimited neoplastic progression due to insufficient immunological anti-neoplastic cell reaction.
4

Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue

51%
Ceccopieri C., Skonieczna J., Madej J. P.
Journal of Veterinary Research
|
2021
|
tom 65
|
nr 1
5

Accessory genital glands in the New Zealand White rabbit: a morphometrical and histological study

51%
Skonieczna J., Madej J. P., Bedzinski R.
Journal of Veterinary Research
|
2019
|
tom 63
|
nr 2
6

Budowa i rola DBP [Vitamin D-Binding Protein] w stanach fizjologii i patologii u zwierzat i ludzi

51%
Madej J. P., Boratynski J., Nowacki W.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 09
987-991
The vitamin D-binding protein (DBP), also known as group-specific component or Gc globulin, demonstrates a pleiotropic effect in human and animal bodies. DBP binds vitamin D and its derivatives. It plays a key role in the extracellular actin-scavenger system (EASS) through the ability of actin binding. DBP is a precursor of the macrophage-activating factor in the inflammation-primed macrophage activation cascade. It binds various cells to the surface of and can significantly enhance the leukocyte chemotactic activity for C5a and C5a des Arg. Anti-human DBP serum shows a cross-reactivity with the serum of the various species in double immunodiffusion or immunoelectrophoresis tests. The polymorphism of DBP in humans and animals indicates a species-characteristic pattern in PAGE and IEF. Gc globulin may be used as a non-specific marker of diseases and some clinical states of patients.
7

Similarity of vitamin D-binding protein (DBP) between various animal species in double immunodiffusion and western blotting

51%
Madej J. P., Boratynski J., Nowacki W.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2007
|
tom 10
|
nr 3
8

Przebieg procesu zapalnego w narządach immunologicznie uprzywilejowanych

51%
Madej J. A., Madej J. P., Kandefer-Gola M.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 06
Organs in which inflammation goes by different rules through protecting them against damage are called immunologically privileged. These include the central nervous system, the front chamber of the eye (excluding the cornea), the liver, the kernel, the pregnant uterus, hamster cheek pouches and hyaline cartilage. The brain immunological privilege is due to fact that it has no lymphatic vessels and contains a small number of T-lymphocytes and macrophages, while a blood-brain barrier is present. In the brain, there is a long-term graft survival of allo-and xenografts, such as the adrenal medulla tissue or fetal tissue allografts. The anterior chamber of the eye is also an immunologically privileged area, which is often a convenient place for grafts of experimental tumors in animals. Inside the testes tolerance of autoantigens present in the germ cells is attained, which is maintained by the presence of incomplete barriers limiting access to reproductive cell antigens of immune cells and antibodies. In other parts of the body such a response to an antigen is not found, which makes these organs very interesting from the point of view of pathogenesis and immunity.
9

Oznaczanie testem ELISA poziomu DBP w surowicy klaczy po porodzie

51%
Madej J. P., Borkowski J., Boratynski J., Nowacki W.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 05
707-712
The vitamin D-binding protein (DBP), also known as Gc-globulin, plays a role as vitamin D and its metabolites carrier; actin scavenger, a precursor of macrophage activating factor (DBP-MAF), binds fatty acids located on the surface of many cell types and enhances chemotactic activity of C5 and C5a des Arg. In the authors’ studies, in order to assess DBP level in mares sera, the ELISA test was developed and standardized. The aim of this study was likewise to compare the level of Gc protein in sera of mares after delivery as compared to non-pregnant mares. The sera derived from 77 full blood mother-mares taken at 36-48 h after delivery, sera of 6 mares that had not taken part in reproduction and sera of 3 geldings the Wielkopolska breed. The authors demonstrated that two days after delivery the DBP concentration in mother-mares sera was significantly higher (p = 0.001291 < α = 0.05) than in non-pregnant mares. It was also proved that DBP level in mother-mares sera had no relation to age or number of deliveries.
10

Parallel manifestation of various types of tumours in canine testes

51%
Nowaczyk R., Nowak M., Madej J. P.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2007
|
tom 10
|
nr 3
11
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An immunohistochemical analysis of lymphocytic infiltrations in canine skin cancers

51%
Madej J. A., Madej J. P., Dzimira S., Nowak M.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 1
Lymphocytic infiltrations located in the extracellular matrix often accompany canine skin cancer. They can be characterised as an inflammatory infiltration and/or a second tumour – lymphoma. The aim of this study was an immunohistochemical analysis of a lymphocytic infiltration which accompanies spontaneous skin cancer. Twenty basal cell carcinoma, 20 non-keratinizing squamous cell carcinoma, 20 keratinizing squamous cell carcinoma and 8 sebaceous gland carcinoma samples which were accompanied by a lymphocytic infiltration and/or secondary lymphatic follicles were verified histopathologically. The expression of bcl-2, CD3, CD79α, Ki-67, MCM-3 and MCM-7 in the lymphocytic infiltration was evaluated. Four types of lymphocytic infiltrations were found: I – diffuse bcl-2⁺, II – diffuse bcl-2⁻, III – follicular bcl-2⁺/⁻ where the centre was bcl-2⁻, and the marginal zone of the follicles and the extrafollicular area were bcl-2⁺ and IV – aggregated bcl-2⁺, where the centre and periphery were bcl-2⁺. The I and IV type corresponds to lymphoma, II type is non-neoplastic immune response and III type suggest reactive follicular hyperplasia. The proliferation of lymphocytes which demonstrated the expression of neoplastic markers (I and IV), suggests preneoplastic phase (pseudolymphoma) or lymphoma – the second independent tumour. A high proliferative index of the follicular blc-2⁺/⁻ follicular infiltration indicates an increased immunological response of the host against skin cancer.
12
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Morphology of immune organs after very virulent plus strain of Marek’s disease virus infection in vaccinated hens

51%
Madej J. P., Wozniakowski G., Gawel A.
Polish Journal of Veterinary Sciences
|
2016
|
tom 19
|
nr 2
Marek’s disease (MD) outbreaks in poultry flocks may be associated with overriding of vaccine immune protection by very virulent (vvMDV) or very virulent plus (vv+MDV) strains. This paper presents the study on lymphoid organ morphology in the latent phase of MD caused by vv+MDV which break post-vaccinal protection in hens. We also immunohistochemically examined B and T populations as well as B/T and CD4+/CD8+ ratio of lymphocytes in lymphatic organs and, as a background, in MD lymphomas from non-lymphatic organs. The number of antigen expressed cells was evaluated as a percentage of positive cells in the one power field. Organ samples were collected from 24 dead reproductive hens (Ross 308 line) in age between 35-56 weeks, infected with vv+MDV. The hens originated from farms with MD outbreaks, despite earlier routine vaccination with CVI988/Rispens + HVT. The control organ samples originated from 15 clinically healthy hens at the same age and line, subjected to the same vaccination schedule. The number of CD3+, CD8+ and TCRγδ+ cells was significantly lower in MDV infected thymus, spleen and cecal tonsils in comparison to that found in the control organs. The proportion of CD4+ was also distinctly reduced in the thymus and limited in the spleen of MDV infected hens. This study revealed that infection with field vv+MDV isolates might break post-vaccinal protection and influence the central and peripheral immune system. The decrease in CD8+ and TCRγδ+ cell number in the thymus, spleen and cecal tonsils suggests that primarily these cells are involved in cell-mediated cytotoxicity against MDV transformed cells during latency.
13

Involvement of CD8plus cells in protective mechanisms in canice mammary adenocarcinomas [short communication]

51%
Nowak M., Madej J. P., Rossowska J., Madej J. A.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 3
The study aimed at demonstration of involvement of CD8+ cells in protective mechanisms in canine mammary gland carcinomas. Material for the studies involved metastases to lungs sampled at autopsy from crossbreed bitches aged 11 to 13 years. The tumours were verified histopathologically as adenocarcinomas. The presence of CD8+ cells infiltrating the examined tumour tissues was estimated in cryo-sections by immunofluorescence methods. Parental tumours free of metastases were accompanied by slight infiltration of CD8+ cells. In the case of metastasing tumours, and especially in their pulmonary metastases, the increased number of CD8+ cells was observed. Taken together, metastasing potential of canine mammary adenocarcinomas was positively correlated with the number of CD8+ cells in the tumour stroma.
14

Correlation between expressions of hypoxia -inducible factor (HIF-1α), blood vessels density, cell proliferation, and apoptosis intensity in canine fibromas and fibrosarcomas

45%
Madej J. A., Madej J. P., Dziegiel P., Pula B., Nowak M.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 1
The study aimed to demonstrate the expression of hypoxia-inducible factor (HIF-1α) in soft tissue mesenchymal tumours (fibroma and fibrosarcoma) in dogs. An attempt was made to correlate the obtained results with density of blood vessels (expression of von Willebrand Factor, vWF), expression of Ki-67 proliferation antigen, and with intensity of apoptosis in studied tumours. The study was performed on paraffin sections of 15 fibromas and 40 fibrosarcomas sampled from 55 female dogs aged 6 to 16 years. Immunohistochemical staining against HIF-1α, vWF, and Ki-67 was performed. Apoptosis was detected with the use of TUNEL reaction. A significantly higher HIF-1α expression was noted in fibrosarcomas in comparison to fibromas (P < 0.0001). HIF-1α expression in fibromas manifested strong positive correlation with tumour vascularity (r = 0.67, P = 0.007). Moreover, HIF-1α expression in fibrosarcomas manifested a moderate positive correlation with tumour malignancy grade (r = 0.44, P = 0.004), tumour vascularity (r = 0.52, P < 0.001), Ki-67 antigen expression (r = 0.42; P = 0.007), and TUNEL- positive cells (r = 0.37, P = 0.017). Expression of HIF-1α was detected in 86.7% of fibroma type tumours and in 100% of fibrosarcomas. In all studied tumours expression of HIF-1α manifested positive correlation with the density of blood vessels, and in fibrosarcomas it correlated also with malignancy grade, intensity of Ki-67 expression, and with intensity of apoptosis in tumour cells.
15

The relationship between concentrations of vitamin D-binding protein (DBD) in serum and colostrum of mares and in serum of their foals in the neonatal period

39%
Madej J. P., Nowacki W., Boratynski J., Borkowski J., Wlodarczyk-Szydlowska A., Musial E.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 4
Vitamin D-binding protein (DBP) participates in the actin scavenger system, it is a carrier of vitamin D and its derivatives, it manifests the capacity to bind mainly monounsaturated and saturated fatty acids, it binds to the surface of several cells and enhances chemotactic activity of C5a of the complement. The present study was aimed at answering the question whether serum DBP level in mares is related to levels of this protein in colostrum and in serum of its progeny. For this purpose, sera from 77 mares, colostra from 72 mares and sera from 69 Thoroughbred foals were collected. Mother’s age, number of deliveries experienced in the past, month of delivery, feeding of foals with colostra were recorded. Blood of the foals was sampled from the umbilical vein during delivery (0h) and 36-48 h after delivery from the external jugular vein, colostra of the mares were obtained after delivery and blood of the mares was sampled 36-48 h after delivery. Concentration of DBP was estimated by a self-designed ELISA. In the present study, DBP concentrations in newborn’s serum were found independent of their concentrations in mother’s serum, her age and number of parities experienced in the past. Colostrum DBP level was found to be lower than that in the mare’s serum and was not correlated to the concentration of this protein in mare’s serum. There was no effect of colostrum feeding on DBP level in the foal serum. These results indicate that serum DBP concentration in newborn foals depends on factors which act directly on the foal. Because of the lack of correlation between plasma and colostrum concentrations of DBP, it can be assumed that DBP is synthesised in the mammary gland and/or specific transport mechanisms exist in the mammary gland.
16
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Podocyturia jako nowy marker w diagnostyce uszkodzeń ciałek nerkowych w medycynie weterynaryjnej

33%
Szczepankiewicz B., Paslawska U., Slawuta P., Madej J. P., Nowak M., Bachor R., Marycz K., Gebarowski T., Czyzewska-Buczynska A., Szewczuk Z.
Życie Weterynaryjne
|
2019
|
tom 94
|
nr 10
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