Cathepsin LI (CatLl) is one of the major molecules in the excretory-secretory products of Fasciola hepatica and is secreted by all stages of the developing parasite; it is involved in tissue penetration, immune evasion, feeding and pathogenesis. Our aim in this study was to clone and characterise the F. hepatica CatLl gene from a Turkish isolate. This is the first report of cDNA encoding CatL 1 protein of F. hepatica from a Turkish isolate. Phylogenetic analysis based on the CatLl showed that the Turkish isolate is genetically related to Asiatic isolates. The cathepsin LI gene may be used for DNA vaccination and recombinant protein derived from the gene can be used for serological diagnosis against F. hepatica in Turkey.