Wyniki wyszukiwania

1

Caspase-like proteases and their role in programed cell death in plants

100%

Piszczek E., Gutman W.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 5

The investigations performed over recent few years have proved the existence of caspase-like proteases in plants. Three groups of caspase-like proteases: metacaspases, legumain family proteases (VPEs) and saspases have been identified and characterized in plants so far. A considerable amount of evidence supports the role of these enzymes in programmed cell death (PCD) occurring during plant development, their organ senescence as well as hypersensitive response (HR) after pathogen attack. Current knowledge of these enzyme molecular and biochemical structures is summarized in the paper. The homology of caspase-like proteases to animal caspases has been also indicated. Some future perspectives of research concerning the signal pathway during PCD, the regulation of activity and mode of action of these proteases are presented in the article.

2

Receptory jądrowe PPAR jako działania leków w zaburzeniach metabolicznych

80%

Stolarczyk M., Gutman W., Derlacz R. A.

Postępy Biochemii

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2011

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tom 57

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nr 2

3

The construction and characteristics of a BAC library for Cucumis sativus L. 'B10'

71%

Gutman W., Pawelkowicz M., Woycicki R., Piszczek E., Przybecki Z.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 1

74-91

Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Cellular and Molecular Biology Letters

1 Postępy Biochemii

3 Gutman W.

2 Piszczek E.

1 Derlacz R. A.

1 Pawelkowicz M.

1 Przybecki Z.

1 Stolarczyk M.

1 Woycicki R.

1 2011

1 2008

1 2007

Caspase-like proteases and their role in programed cell death in plants

Receptory jądrowe PPAR jako działania leków w zaburzeniach metabolicznych

The construction and characteristics of a BAC library for Cucumis sativus L. 'B10'