A bacteriocin divercin was produced in batch cultures by Carnobacterium divergens. The bacteria were grown in the liquid MRS media containing enzymatic hydrolysates of whey, casein or malt roots as organic nitrogen sources. The divercin production was greatly affected by the medium composition. The highest divercin activity (12,800 AU/mL), measured by the critical dilution assay using C. piscicola as an indicatory microorganism, was obtained in the medium containing whey, casein hydrolysates, and glucose. Omission of glucose in this medium delayed the divercin biosynthesis only for 5 h. The medium developed was more efficient than the MRS medium used commonly for the bacteriocin production.
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