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Bile acid transport in hypercholesterolemic resistant rabbits

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Izzat N. N., Overturf M., Weisbrodt N. W., Loose D. S.

Journal of Physiology and Pharmacology

2009

tom 60

nr 3

79-84

We examined bile acid transport and expression of the apical sodium-dependent bile acid transporter (ASBT) in ileal preparations to determine if alterations in bile acid excretion contributed to a hypercholesterolemia-resistant phenotype in rabbits (CRT/mlo). Taurocholate transport was not different between normal (NR) and CRT/mlo rabbits fed regular diet. However, feeding cholesterol-enriched diet reduced taurocholate transport significantly in CRT/mlo rabbits (0.53±0.06 pmol/µg protein) compared to regular diet (0.95±0.14 pmol/µg protein), but had no effect in NR rabbits. Cholesterol-enriched diet increased ASBT mRNA in CRT/mlo (2.6 ± 0.7 to 5.4 ± 0.1); no significant changes occurred in NR. Some CRT/mlo rabbits carry a polymorphism in ASBT at amino acid 333 (P333L). In transfected HEK293 cells, TC transport of P333L allele was significantly lower (0.08 ± 0.01 vs 0.13 ± 0.01 pmol/µg protein/15 sec, P< 0.05). This allele was not found in NR rabbits. The data suggest that the phenotype of the CRT/mlo rabbit is due to changes in bile acid transport as well as bile acid metabolism.

Ograniczanie wyników

1 Journal of Physiology and Pharmacology

1 Izzat N. N.

1 Loose D. S.

1 Overturf M.

1 Weisbrodt N. W.

1 2009

Wyniki wyszukiwania

Bile acid transport in hypercholesterolemic resistant rabbits