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The present work is designed to investigate the concentrations of heavy metals (Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb) and physico-chemical properties including pH, conductivity, salinity, total dissolved solid (TDS), dissolved oxygen (DO), and specific gravity that were analyzed in fresh bovine milk samples sold in various towns of District East, Karachi. Statistical techniques, including cluster analysis and principal component analysis (PCA), were performed to organize the recorded data in such a way as to expose the internal structure of multivariate data matrix. Results of PCA revealed that conductivity, salinity, and TDS possessed strong relationships with each other. The levels of heavy metals were assessed by atomic absorption spectroscopy (AAS). The mean concentrations of Cr, Mn, Fe, Co Ni, Cu, Zn, Cd, and Pb were assessed as 0.001-0.232 mg/l, 0.001-0.460 mg/l, 0.001-3.260 mg/l, 0.001-0.108 mg/l, 0.001-0.122 mg/l, 0.001-0.098 mg/l, 2.086-5.498 mg/l, 0.001-0.053 mg/l, and 0.001-1.428 mg/l, respectively. Remarkable numbers of samples were found to be contaminated with Cd and Pb and exceeded the tolerable guidelines set by standards.
Efficacy of two strains of Pseudomonas aeruginosa (Pa-5 and IE-2) and aBacillus subtilis isolate alone or in conjunction with neem cake or Datura fastuosa was tested for the management of three soilborne root-infecting fungi including Macrophomina phaseolina, Fusarium solani and Rhizoctonia solani and the root-knot nematode, Meloidogyne javanica on uridbean. Biocontrol bacteria used in combination with either neem cake or D. fastuosa gave better control of the root-rot and root-knot infection with the enhancement of growth of uridbean compared to the use of either component alone. Neem cake 1 % w/w mixed with P. aeruginosa strain lE-2 caused greatest inhibition of the root-knot development due to M.javanica. P. aeruginosa and B. subtilis used with organic amendment also increased Bradyrhizobium-nodules in the root system.
Soil amendments with powdered seeds of Trigonella foenum - graecum (fenugreek) caused soil suppressiveness against Meloidogyne javanica. Decomposed seeds of fenugreek caused marked reduction in nematode population densities and subsequent root-knot development as compared to the aqueous extract of the seeds indicating that some indirect factors are involved in the suppression of root-knot nematode. Both decomposed seeds and aqueous extracts enhanced plant height and fresh weights of Dat whereas root growth remained uninfluenced. Changes in fungal communities associated with nematode control were studied by comparing population numbers of :ngi in the soil and in internal root tissues (endorhiza) in non-amended and fenugreekanded soils. Acremonium sp., Chaetomium globosum, Fusarium solani, Macropho'na plwseolina and Rhizoctonia solani were found to colonize inner root tissues of ugbean. Acremonium sp., C. globosum and F.solani were isolated in a relatively her frequency from roots growing in the amended soils while M. phaseolina and R.solani colonized greatly in roots growing in non-amended soil. Of the fungi isolated soils, Penicillium brefaldianum caused maximum juvenile mortality of M. javanica whereas F.solani caused greatest inhibition of egg hatch.
The potential of 3 Pseudomonas aeruginosa strains as biocontrol agents of rootinfecting fungi Macrophomina phaseolina, Flusarium solani and Rhizoctonia solani and the root-knot nematode Meloidogyne javanica Was tested on chi!i and uridbean under greenhouse conditions. All the three strains significantly reduced nematode populations in soil, invasion, multiplication and gall formation due to M. javanica. Root infection by fungi was also effectively suppressed following P. aerllginosa application. Bacterial antagonists exhibited better biocontrol and growth promoting activity in 15-day-old plants than did those harvested at 30 or 45 days. Population of the bacterium in the rhizosphere declined rapidly after 15 days of nematode inoculation. Strain Pa-5 showed maximum nodulation in 15-day-old samplings while strain Pa-7 showed highest number of nodules in 30 and 45-day-old uridbean plants.
The potential impact of Pseudomons aeruginosa strain IE-6 as abiological control agent against Meloidogyne javanica at four inoculum densities (0, 250, 500 and 1000 eggs/plant) and Rhizoctonia solani at three inoculum levels (0, 1 and 3 mi culture suspension/kg of soil) was examined on tomato in the greenhouse experiments. The biocontrol bacterium suppressed root infection caused by R. solani and M. javanica on tomato in both sterilized and non-sterilized soils. Root-rot infection increased with the increase in pathogen(s) concentration. P. aeruginosa showed better biocontrol effects at low population levels of M.javanica and R. solani than at higher population densities of the pathogen(s). Root-rot disease severity was more pronounced in sterilized soil compared to the non-sterilized one. Soil infested with high population densities of R. solani (3 mi /kg of soil) and M. javanica (2000 eggs/pot) resulted in complete mortality of tomato seedlings in sterilized soil, whereas some plants were found to survive in non-sterilized soil. There seems to be a correlation between population density of M. javanica and root colonization by R. solani. Root colonization by other three root-infecting fungi inc1uding Macrophomina phaseolina, Fusarium oxysporum and Fusarium solani was also lower in the presence of P. aeruginosa in non-sterilized soil. P. aeruginosa enhanced plant growth in both types of soil.
Effect of various fungicides on the efficacy of Pseudomonas aeruginosa, a plant growth-promoting rhizobacterium in the control of root-infecting fungi such as Macrophomina phaseolina, Fusarium oxysporum and F. solani on four different varieties of wheat was evaluated under field conditions. Bayleton (aj. triadimephon), Bavistin (aj. carbendazym) and Topsin-M (aj. thiophanate-methyl) reduced bacterial survival on wheat seeds whereas Benlate (aj. benomyl) was not effective in this respect. P. aeruginosa used in combination with Benlate showed effective control of soilborne root-infecting fungi along with the enhancement of growth and grain yield of wheat.
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