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  1. 14 Medycyna Weterynaryjna

  2. 12 Polish Journal of Veterinary Sciences

  3. 4 Annales Universitatis Mariae Curie-Skłodowska. Sectio DD:Medicina Veterinaria

  4. 3 Bulletin of the Veterinary Institute in Puławy

  5. 2 Journal of Elementology

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  1. 46 Puchalski A.

  2. 29 Urban-Chmiel R.

  3. 28 Wernicki A.

  4. 18 Dec M.

  5. 9 Mikucki P.

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  1. 1 2022

  2. 3 2019

  3. 1 2018

  4. 1 2017

  5. 4 2015

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1

Use of oromucosally administered interferon-alpha in the prevention and treatment of animal diseases

100%
Dec M., Puchalski A.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 2
175-186
Interferon-a (IFN-a) is well known as a clinically effective antiviral and antineoplastic therapeutic agent. It has also been shown to have immunoregulatory properties. IFN-a stimulates a cell-mediated innate immune response and then participates in the transition of the initial host innate response to an effective adaptive immune response. IFN-a is produced in small quantities in nasal secretions during viral infections, prompting many authors to suggest that low-dose oromucosal administration of IFN-a effectively mimics nature. Moreover, the injectable high-dose interferon therapy currently approved for various human disorders causes numerous side effects. By contrast, oromucosal administration of IFN-a is not associated with toxic effects. Another distinct advantage is ease of administration: the IFN can be dissolved in drinking water or administered by nebulization to the oral or nasal cavity. This review describes the current state of knowledge concerning orally administered IFN-a, of both human and animal origin, as a prophylactic or therapeutic agent in veterinary medicine. We present the effects of IFN-a in such animals as cattle, pigs, horses, cats, dogs and chickens, and attempt to explain its mechanism of action following oromucosal administration. It is hoped that this review of the medical literature on the use of IFN-a in animals will give practitioners a better understanding of the challenges and benefits of using this interesting cytokine in clinical practice.
2

Potential interaction between the Cepaea nemoralis wild snail and Citrobacter spp. bacteria

100%
Kowalczyk-Pecka D., Puchalski A.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 06
The aim of this study was to determine the effect of various dietary supplements on the level of selected unsaturated fatty acids, with potential antibacterial properties, in the hepatopancreas of Cepaea nemoralis snails, and to perform a preliminary phenotypic characterization of Citrobacter braakii strains occurring in the intestinal tract of these snails, which are environmental reservoirs for these bacteria. Gas chromatography was used for fatty acid analysis of the snails. Biochemical characteristics of Citrobacter braakii isolates and their resistance profiles for six chemiotherapeutics were determined. Giving Cepaea nemoralis food supplemented with Allium sativum (garlic), Urtica dioica (stinging nettle) and Aloe vera caused an increase in the total unsaturated fatty acids in the hepatopancreas. In the invertebrates studied, the Aloe vera and Allium sativum supplements caused an increase in arachidonic acid, while Utrica dioica increased oleic and linoleic acid content, which probably contributed to the 2-log CFU (colony forming unit) reduction of Citrobacter in the intestine. Wild snails of the genus Cepaea should be taken into consideration in epidemiological investigations as potential vectors for pathogenic strains of Citrobacter spp.
3

Wartosc rzezna i jakosc miesa loszek czterech ras, pochodzacych po rodzicach importowanych z Danii

81%
Wajda S., Okruszek A., Puchalski A.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
|
1998
|
tom 53
33-41
4
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Assessment of the influence of fluoride, modified transdermal replacement hormone therapy and supplement hormone therapy on unmanageable osteoporosis in postmenopausal women

81%
Stanosz M., Stanosz S., Puchalski A.
Journal of Elementology
|
2009
|
tom 14
|
nr 3
545-551
The study was conducted on 40 women in the early postmenopausal period, aged 52.3±3.1 years with primary osteoporosis unmanageable in treatment, divided into 2 groups based on a randomized list. Group I (n-20) was administered orally fluoride 0.25 mg kg-1 24 h-1 with modified transdermal hormone therapy/HRT, and group II (n-20) was administered orally fluoride and supplement hormonal therapy(HST) in 21 therapeutic cycle. The serum concentrations of osteocalcin (OC), procollagen(PICP), insulin-like growth factor I (IGF-1), prolactin basic (PRL) and prolactin after metoclopramide (PRL/ MCP) 4 times by using radioimmunoassy methods, before treatment and after 1, 3, 12 months of treatment. Bone mineral density (BMD) L2 – L4 was determined before treatment and at 12 month with a dualenergy x-ray absorptiometry scanner (Lunar DPX-1Q). In group I women receiving fluoride and transdermal HRT IGF-1 increased significantly while the concentrations of OC and PICP significantly decreased after 3 and 12 months of treatment but no statistically significant changes in the PRL concentration occurred. In group II women receiving orally fluoride and HST, a significant decrease in the concentration of IGF-1, OC after 3 and 12 months and a significant increase in the concentration of PRL and PRL/ MCP after 1, 3 and 12 months of treatment compared with the baseline values appeared. The concentration of type I procolagen (PICP) showed no statistically significant changes. Increase in bone mineral density was statistically significant L1, L2 (p < 0.05), L3, L4 (p < 0.01) compared with the baseline in the group receiving transdermal HRT. In women receiving fluoride and orally HST increase in the bone mineral density for L1 and L2 was non-insignificant, whereas for L3 and L4 it was significantly higher compared with the baseline (p < 0.05).
5

Characterization of Escherichia coli strains associated with canine pyometra

81%
Wernicki A., Krzyzanowski J., Puchalski A.
Polish Journal of Veterinary Sciences
|
2002
|
tom 05
|
nr 2
6
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An electrophoretic characterization of iron-transporting proteins in Mannheimia haemolytica A1

81%
Puchalski A., Urban-Chmiel R., Dec M., Wernicki A.
Polish Journal of Veterinary Sciences
|
2013
|
tom 16
|
nr 3
Iron-regulated outer membrane proteins (IROMPs) in Mannheimia haemolytica A1, which function as a receptor for complexes containing iron ions, are induced by iron deficiency in the growth environment of the bacteria. Densitometric analysis of SDS-PAGE separation showed expression of IROMPs of 71, 77, and 100 kDa in the case of bacteria grown in a medium with 2,2-dipyridyl. The electrophoregrams obtained in 2-DE separations confirmed the presence of protein fractions with these molecular weights and isoelectric points ranging from 5.4 to 6.4. The results of the study also confirmed the ability of M. haemolytica A1 proteins involved in iron uptake to induce a protective immune response. In Western blot with serum from convalescent calves naturally infected with M. haemolytica A1, distinct reactions were obtained for IROMPs of 71, 77, and 100 kDa.
7

Wplyw stresu transportowego na stopien wrazliwosci leukocytow na dzialanie leukotoksyny Mannheimia haemolytica

81%
Urban-Chmiel R., Puchalski A., Wernicki A.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 09
1045-1047
The purpose of the study was estimating the viability and susceptibility effect of leukocytes isolated from cattle before and after the transportation in vitro on M. haemolytica leukotoxin (Lkt) cytotoxicity. 40 Simentaler heifers that were transported by truck a distance of 1700 km for 72 hours were used in the experiment. The material for the study was the blood (40 samples) collected on heparin directly before and after transportation. In relation to leukocytes the examination of susceptibility on cytotoxic effect of Lkt has been carried out with the use of MTT (microtitration assay) and the viability of leukocytes after 1, 2, 3 and 6 hour of incubation. The results obtained in the cell viability test did not show statistically significant differences (P≥0.05) in 1st and 2nd hour of incubation in leukocytes obtained from heifers before and after transportation. After the 1st hour of incubation the percentage of leukocyte viability was on a very high level and showed 87% in both groups of animals. The significantly lower cell viability values in comparison to leukocytes isolated from animals before the transportation was observed in the transported heifers from 3rd to 6th hour of incubation. The analysis of the results obtained by MTT test indicated statistically significant differences in the susceptibility of leukocytes for cytotoxic activity of Lkt. The average values of toxic activity of Lkt in relation to leukocytes isolated before and after transportation was 79% and 92% respectively. The lytic activity of Lkt for 50% of the cell population referred as 1 unit (1U) was observed in Lkt concentration 15 mg/ml (leukocytes before transportation) and 7.5 mg/ml (after the transportation). The increase of susceptibility of leukocytes isolated after transportation on the cytotoxic effect of leukotoxin suggest the significant influence of transporting stress on the increase of respiratory diseases caused by M. haemolytica strains.
8

Profile bialek blony zewnetrznej szczepow Mannheimia [Pasteurella] haemolytica, M.glucosida, P.trehalosi oraz szczepow nietypowych

81%
Puchalski A., Wernicki A., Mikucki P., Urban-Chmiel R.
Medycyna Weterynaryjna
|
2002
|
tom 58
|
nr 06
439-443
9

Badania serologiczne w kierunku boreliozy psów na Lubelszczyźnie

81%
Adaszek L., Winiarczyk S., Puchalski A.
Annales Universitatis Mariae Curie-Skłodowska. Sectio DD:Medicina Veterinaria
|
2008
|
tom 63
|
nr 1
10

Ocena humoralnej odpowiedzi immunologicznej cielat na przeciwciala bialek blony zewnetrznej [OMPs] Mannheimia haemolytica serotyp 1

81%
Wernicki A., Puchalski A., Urban-Chmiel R., Mikucki P.
Medycyna Weterynaryjna
|
2002
|
tom 58
|
nr 11
860-862
11

Antybiotykowrazliwosc oraz profil plazmidowy szczepow Pasteurella haemolytica

81%
Wernicki A., Puchalski A., Urban-Chmiel R.
Medycyna Weterynaryjna
|
1999
|
tom 55
|
nr 09
623-626
12

Profile bialkowe preparatow antygenowych uzyskiwanych ze szczepow Malassezia pachydermatis

81%
Ziolkowska G., Nowakiewicz A., Puchalski A.
Medycyna Weterynaryjna
|
2004
|
tom 60
|
nr 10
1118-1123
13

Restriction endonuclease analysis of Salmonella enterica ser. Enteritidis full genome by means of pulsed-field gel electrophoresis in FIGE modification

81%
Kowalczyk D., Wernicki A., Pejsak Z., Puchalski A.
Polish Journal of Veterinary Sciences
|
2001
|
tom 04
|
nr 3
14

Comparison of immunogenic properties of native and inactivated Mannheimia haemolytica Lkt in calves

81%
Wernicki A., Mikucki P., Urban-Chmiel R., Puchalski A.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 2
83-88
The aim of this study was to compare the immunostimulatory properties of Lkt of M. haemolytica inactivated by formaldehyde and glutaraldehyde and to evaluate the neutralizing properties of anti-Lkt antibodies. The experiment was conducted on 20 Black-and-White Lowland calves of 100 kg body weight, assigned to 4 experimental groups. The animals were given subcutaneous vaccine injections with native Lkt, Lkt inactivated by formaldehyde or Lkt inactivated by glutaraldehyde. The anti-Lkt antibody titres were measured using an enzyme-linked immunosorbent assay (ELISA), based on absorb- ance of the sera obtained from the animals immunized with the different forms of Lkt. The protective effects of the antibodies present in the sera isolated from the vaccinated animals were estimated using an MIT assay. Analysis of the ELISA absorbance values in the sera from calves in the vaccinated groups did not show any significant differences between the groups. The highest increase in absorbance of sera was observed in calves from the group that received formaldehyde-inactivated Lkt. In the case of calves immunized with native Lkt, the absorbance values were lower than in the group immunized with Lkt inactivated by formaldehyde. The lowest absorbance values were observed in sera obtained from calves vaccinated with Lkt inactivated by glutaraldehyde. Analysis of the MTT assay results revealed the greatest Lkt-neutralizing properties of antibodies in the sera of calves immunized with two doses of a vaccine containing native Lkt and Lkt inactivated with formaldehyde.
15

Evaluation of the influence of transport and adaptation stress on chosen immune and oxidative parameters and occurrence of respiratory syndrome in feedlot calves

81%
Wernicki A., Urban-Chmiel R., Puchalski A., Dec M.
Bulletin of the Veterinary Institute in Puławy
|
2014
|
tom 58
|
nr 1
Immune and oxidative parameters were evaluated as indicators of the influence of stress on the occurrence of respiratory syndrome in feedlot calves. The study was carried out on 60 2 and a half-month-old Simmentaler calves transported at feedlot. Mean daily feed consumption, daily weight gain, and behavioural activity were evaluated. Blood was collected on days 1, 3, 7, 14, 21, and 28 of feedlot. Serum NO ions, lipid peroxidation, acute phase proteins, IgG, and IgM were determined. The calves showed a decrease in feed consumption during the first 7 d of the feedlot. Sporadic stereotypies were noted. NO concentration showed a significant (P < 0.05) increase on days 7 and 14. Concentration of thiobarbituric acid reactive substances (TBARS) increased (P < 0.05) on days 1 and 3. IgM concentration was found to be considerably lower on days 14 and 28. Serum haptoglobin level showed a significant increase in stressed calves on days 1, 3, 7, and 14 of the leedlot. Based on these results, it can be suggested that stressors associated with transport and adaptation to the feedlot induce a stress reaction in calves, resulting in behavioural disorders, reduced weight gain, suppression of the humoral immunity and increased morbidity during the first weeks. These parameters seem to be crucial in evaluating the animals' health and welfare.
16

Skutecznosc natywnej i inaktywowanej leukotoksyny Mannheimia haemolytica w immunoprofilaktyce swoistej syndromu oddechowego owiec

81%
Mikucki P., Wernicki A., Puchalski A., Urban-Chmiel R.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 11
1302-1305
The purpose of this study was to compare the immunostimulating properties and prophylactic effects of native M. haemolytica leukotoxin (Lkt) which was inactivated by formaldehyde and glutaraldehyde. The study also estimated the neutralizing properties of antibodies induced by different forms of Lkt and their protective effects on experimental challenge in sheep. The results of the study suggest that the inactivation procedure has an important effect on immunogenic obtained toxins. Vaccines used in the immunized animals demonstrated an immunogenic presence which was observed in an increase of the specific antibody titers in sera following immunization. An increase in the neutralizing properties of antibodies contained in the sera of animals immunized by Lkt was also observed. The obtained results indicate the possibility of a more effective use of M. haemolytica Lkt isolated from bovine respiratory syndrome as a subunit of the vaccines already used in immunoprophylaxis of this disease in sheep.
17

Jakość mięsa z tusz tuczników zaliczonych do klasy E, U i R w systemie klasyfikacji EUROP

81%
Daszkiewicz T., Wajda S., Puchalski A.
Zeszyty Naukowe. Przegląd Hodowlany
|
2000
|
tom 48
18
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Influence of modified trandermal hormone replacement therapy including magnesium, on bone formation markers with osteoarthrosis of spine in women

81%
Stanosz M., Stanosz S., Puchalski A.
Journal of Elementology
|
2009
|
tom 14
|
nr 2
365-372
In a randomized study 50 women, aged 51.7±2.8 years, suffering from primary osteoarthrosis (OA), were divided into two, equal groups (I, II). The women were employed in garment industry in contract work system. They were working in compulsory, mainly standing position. The women complained of backache of the lumbar region continuing for the minimum 5 years. During the study, bone mineral density (BMD) of the lumbar spine was assessed twice with the densitometry DEXA method (Lunar Corporation equipment). Before treatment, structural changes in the lumbosacral spine were revealed using a CT Simens Sonata Plus 4. One energy technique (SEQCT) was applied. Concentrations of bone-forming markers in serum were measured three times: before treatment and 3 and 12 months afterwards. The concentration of acid phosphatase in serum was assessed by the enzymatic method according to Hitachi. The concentrations of osteocalcin and procolagen were radoimmunologically assessed by means of DRG Company – sets and concentration basal prolactin (PRL) before treatment radioimmunoassy kits produced by bioMerieux. In the first stage of the treatment, the women in the first group received placebo for three months. Slow Mag B6 was administered for three months to the women in the second group. In the second stage of the treatment, the women in both groups received 21-day therapeutic cycles of modified transdermal hormonal replacement therapy. Additionally, bromocriptine (2.5 mg per day) and Slow Mag B6 (160 mg per day) were administered orally. The cycles repeated at a 7-day interval. During the interval, withdrawal bleeding occurred. The results were statistically assessed by means of computerized programme package Statistica PL, version 5. It was stated that in 60% of women suffering from primary OA the basal concentration of prolactin in serum in was elevated above 25 ng/ml; in 25% women it was on the border level, and in 15% of the patients it was below the lower limit of the normal values. The combined treatment in women suffering from OA caused increase in bone-forming markers and decrease in pathological resorption processes of mineralization of the vertebral bodies. After 12 months of the therapy, resorption in the lumbar spine was diminished compared to the initial values, before the treatment. These changes were significant in L3/L4 vertebral bodies (p<0.05).
19

Charakterystyka wybranych wlasciwosci szczepow E. coli izolowanych z przypadkow ropomacicza suk

81%
Wernicki A., Krzyzanowski J., Puchalski A., Kowalczyk D.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 01
49-52
A total of 128 cultures of E. coli isolated from uterine pus from 143 bitches with a clinical diagnosis of pyometra were examined by biotyping, resistotyping, drug susceptibility, serum resistance and enzymatic activities. Among the isolates a total of 12 different biochemical profiles were distinguished. The majority (92.8%) of the isolates fell into 5 biotypes. From among 20 biochemical features tested with the API 20E System, only six were recognized to induce variations of strains. These are ornithine decarboxylase, fermentation of rhamnose, saccharose, melibiose, sorbitol and inositol. Seven major enzymatic types were defined on the ground of differentiation in activity of esterase (C4), esterase lipase (C8), /З-glukuronidase and a-galactosi- dase. 47 (36.7%) of the E. coli strains were resistant to two or more drugs. However five strains were resistant to 12 or more antimicrobial agents. An analysis of resistance to some chemical agents was evidence of seven resistogram types. The majority (62.5%) of the isolates were included in profile АВЕН. Resistance to bactericidal action of serum was detected in 119 (93%) strains tested.
20

Utilization of leukotoxin as vaccine antigen in immunoprophylaxis of shipping fever in cattle

81%
Wernicki A., Urban-Chmiel R., Puchalski A., Mikucki P.
Polish Journal of Veterinary Sciences
|
2001
|
tom 04
|
nr 4
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