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In the present study, we aimed to investigate for the first time the seroprevalence of feline coronaviruses (FCoV) in Van cats kept in a multiple-cat environment. A total of 24 male and 46 female Van cats aged between two months and nine years were used in the study. FCoV antibodies were determined using the commercial feline coronavirus antibody ELISA kit. The cats were examined clinically before blood sampling. No clinical signs of feline infectious peritonitis diseases were observed in the cats. But, out of the 70 cats, 38 (54.3%) showed seropositivity to FCoV. When the results were examined with regard to gender, 28 (60.9%) of female cats, and 10 (41.7%) of male cats were seropositive. When the results were examined with regard to age, out of the 18 cats aged between two months and one year, three (16%) were seropositive, out of the 10 cats aged between one to two years - eight (80%), out of the 27 cats aged between two to five years - 15 (55.5%), out of the 13 cats aged between five to eight years - 11 (84%), and all cats (two) aged over eight years showed seropositive reaction to FCoV.
In the study, antimicrobial resistance and plasmid profile of Vibrio alginolyticus strains isolated from diseased European sea bass (Dicentrarchus labrax, L.) collected from a commercial farm located on the Agean Region coast of Turkey, were investigated. The agent was identified by classical biochemical tests and the API 20NE system. Bacterial strains were tested for resistance to different antimicrobials by disc agar diffusion method and minimal inhibitory concentration (MIC) at two different incubation temperatures (22 ± 2°C and 28 ± 2°C for 24 to 28 h), and screened for plasmid DNA by agarose gel electrophoresis. It was found that 15 strains were susceptible to kanamycin but they revealed resistance to ampicillin, bacitracin and streptomycin at 22°C. The strains were resistant to bacitracin and streptomycin but 14 strains were found to be intermediately resistant. One strain was resistant to ampicillin at 28°C; two strains showed sensitivity to kanamycin, whereas 12 strains were intermediately resistant, and one strain was resistant to the same antibiotic at 28°C. The strains demonstrared susceptibility to low concentrations of chloramphenicol, kanamycin, and trimethoprim (MICs of ≤ 8 µg/mL) at 22°C. The examined strains were found to be susceptible to chloramphenicol (MICs of ≤ µg/mL) and trimethoprim (MICs of ≤8 µg/ mL) at 28°C. The strains harboured 2-3 plasmids, with sizes ranging from 68 to 126 kb.
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