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Our study was aimed at determining contents of cadmium, lead, and oxalic acid in select wild edible mushrooms harvested from areas potentially more (Ostrowiec Św (P1). and Ożarów (P2)) or less the protection zone of Magurski National Park (M – mountain) exposed to pollution. The experimental material were fruiting bodies of four species of edible mushrooms: boletus (Boletus edulis), bay bolete (Xerocomus badius), red-capped scaber stalk (Leccinum aurantiacum), and slippery Jack (Suillus luteus) collected in late August/early September of 2009. The results of contents of cadmium in the examined mushrooms indicate that the permissible (3 mg·kg⁻¹ d.m.) level was exceeded in fruiting bodies of boletus originating from the polluted (P1, P2) areas and mountain area (M), where its concentration was the highest. Caps and stalks of boletus originating from the protection zone of Magurski National Park were found to contain, respectively, 5.22 mg Cd·kg⁻¹ d.m. and 1.86 mg Cd·kg⁻¹ d.m., on average, (with the value of 3.54 mg Cd·kg⁻¹ d.m. that may be assumed for the whole fruiting body). Taking into a small contribution of mushrooms in a human diet, it does not pose a risk to human health. The concentration of lead in the analyzed mushrooms did not exceed 1.0 mg·kg⁻¹ d.m. The mean content of soluble oxalates in the analyzed species of mushrooms ranged from 35.5 to 104.1 mg·100 g⁻¹ d.m. (per whole fruiting body). Irrespective of the origin, the lowest content of oxalates was reported in fruiting bodies of slippery Jack (35.5-59.1 mg·100 g⁻¹ d.m). Caps of all investigated mushroom species were characterized by ca. 1.6 to 3.1 times higher content of oxalates than the stalks.
Introduction and objective. The objective of this study was to determine the effect of per os administration of 3 various dosages of a Citrosept preparation (a grapefruit extract)to growing turkey hens on changes in their selected haematological and immunological blood indices. An attempt was also undertaken to select the most efficient dose of the preparation with respect to the mentioned indices in turkey hens. Materials and methods. The experiment was conducted on 180 turkey hens allocated at random to 4 groups, 45 birds in each group. Samples of their full blood were analyzed for haematological indices, such as red blood cell count (RBS), haemoglobin content (Hb), haematocrit value (Ht), and white blood cell count (WBC). Samples of blood plasma were assayed to determine the activity of lysozyme (chamber-diffusive method) and heterophils capability to reduce nitro blue tetrazolium (stimulated and spontaneous NBT test). Phagocytic activity of leucocytes against Staphylococcus aureus 209P strain was assessed and expressed as the percentage of phagocytic cells (% PC) and phagocytic index (PI). Results. The administration of the grapefruit extract to turkey hens with drinking water caused a significant increase in haemoglobin content in blood, as well as an increase in non-specific humoral immunity marker (activity of lysozyme) and non-specific cellular immunity marker (percentage of phagocytic cells; P ≤ 0.05). Conclusions. The results obtained enabled the positive evaluation of the advisability of applying the Citrosept preparation in the feeding of turkey hens at the age of 6–9 weeks. Among the doses examined, the most efficient with respect to the stimulation of the non-specific humoral and cellular immunity was the dose of 0.021 ml/kg of body weight.
The aim of this study was to analyze the influence of brewing time on the content of select trace elements (Cu, Zn, Fe, Mn) and heavy metals (Pb, Cd) in homogeneous and mixed herbs used in therapy of digestive system diseases and in regulation of digestive processes. The study involved 10 types of preparations of herbal teas (mono- and multi-component sachets) acting on the gastrointestinal tract and digestive processes. Also, four types of black teas were examined for comparison. Aqueous extracts were made from each sample. As the manufacturers recommend herbal teas to be brewed for 5-15 min, in our study analyses were carried out for aqueous extracts brewed for 5 and 10 min. Contents of minerals in dry matter of the analyzed herbal and black teas and herbal blends were very differentiated, which could be due to species diversity and origin of the raw material. The brewing time had no significant effect on the percentage of the analyzed microelements extraction to the aqueous phase, however in the case of copper, manganese and lead the longer time of extraction resulted in a reduced yield of this process. An opposite dependency was noted in the case of iron, cadmium, and zinc.
The aim of the study was to evaluate the effect of copper (Cu) level in the diet on the distribution, and biological and immunological responses in a rat model. Two experimental groups of rats received a diet containing the recommended (higher) amount (6.5 mg/kg) of CuCO3 (CuH) or a diet containing the reduced (lower) level of Cu (without CuCO3 supplementation; CuL) for 7 or 35 days (T7 and T35, respectively; in total 4 subgroups). Reduced level of Cu caused a decrease in the Cu excretion in faeces and an increase in the Cu level in urine, but in overall the digestibility and utilization indexes of Cu were increased. There was also found a decrease in the Cu content in the plasma and liver in CuL group (results of a 5-day balance tests). After 7 days the reduction of Cu dose caused a decrease in plasma contents of Cu, Zn, Fe, MI (%), GRA (109 /l), IgA and IgM; on the other hand the plasma contents of Mg, WBC, LYM (%) and BIL, and activities of ALP and GGT were increased. The elongation of the feeding period from 7 to 35 days in the CuH group caused a decrease of Zn, P, IgA, BIL, TC, TG plasma contents, and activity of ALT, LDH and an increase in WBC, LYM (%), MID (109 /l), MI (%), GRA (%), RBC, HB, IgM, IL-6, GLU, UA, ALB and HDL and activities of AST, ALP and GGT. However when the CuL diet was elongated, decreased plasma contents of Cu, IgA, IgM, IL-6, GLU, ALB and BIL, and activity of GGT; and increased plasma contents of Zn, WBC, LYM (%) and GRA (109 /l), and activity of ALP were stated in comparison to CuH group. The results of the study suggest that rats partially develop adaptive mechanisms, thanks to which are able to function at a reduced level of Cu in the diet. But, reduced level of Cu in the diet may interfere with the immune response and BIL and ALB metabolisms, playing an antioxidant role in the body. Furthermore, it can be indicated that the level of many blood indicators may depend on the growth of rats, therefore it may not be assumed that the supplementation of Cu in rats is completely unnecessary
The aim of this study was to investigate the influence of Citrosept preparation and Scutellaria baicalensis root extract, administered per os to growing turkey hens in 3 different dosages, on the content of selected mineral elements in blood plasma of slaughter turkey hens. An attempt was also made to specify the most effective dosage of the applied preparations with the highest efficiency regarding increased levels of examined macro- and microelements in the birds’ blood. The research experiment was conducted on 315 turkey hens randomly divided into seven groups, each consisting of 45 turkey hens. Group C constituted the control group without experimental additions of the above-mentioned preparations. In turkey hens in groups II–IV, Citrosept preparation was instilled to water in the following dosages: Group II – 0.011 ml/kg of bm; Group III – 0.021 ml/kg of bm; Group IV – 0.042 ml/kg bm. For birds which belonged to groups V–VII, a preparation of Scutellaria baicalensis root extract was instilled to water in the following dosages: Group V – 0.009 ml/kg of bm; Group VI – 0.018 ml/kg of bm, Group VII – 0.036 ml/kg bm. In the examined plant extracts and blood plasma of the birds the levels of Na, K, Ca, Mg, Cu, Zn, and Fe were identified. The use of examined extracts influenced changes in the levels of all tested elements in slaughter turkey hens’ blood plasma. An upward tendency was recorded in the level of calcium and magnesium, and a downward tendency of sodium, potassium, copper, zinc, and iron in relation to the results achieved in the control group.
The aim of this study was to determine the content of basic nutrients as well as selected macroand micro-elements in the albumen and yolk of eggs produced at large-scale, commercial poultry production farms (10) as well as in organic (8) and courtyard farms (12). Ten eggs were randomly collected 3 times on each farm. For chemical analyses, the eggs were hard-boiled for 15 min and then stored at a temp. of 4°C until analyzed. The albumen and yolk of hard-boiled eggs were assessed for the content of dry matter, total protein, crude fat and crude ash, and for their energy value (net Atwater equivalents) as well as the concentrations of K, Na, Ca, Mg, P, Fe, Zn, Cu, Se and Mn. No differences were observed in the content of dry matter, total protein and crude ash. However, differences were demonstrated for the crude fat content, the highest (P < 0.05) level of which was found in eggs from the organic system (higher by 60% in albumen and by 17% in yolk than in the other eggs), which was accompanied by an increased energy value of these eggs. In all the analyzed eggs, similar concentrations were noted for Mg (egg white) and P (egg albumen and yolk). The albumen of eggs from the organic system was characterized by the highest (P < 0.05) content of K, Na, Ca, Zn, Se and Mn. In turn, the albumen of eggs from large-scale commercial poultry production farms contained the highest (P < 0.05) levels of Fe, Cu and Se. Yolks of the eggs from the organic system accumulated the highest (P < 0.05) levels of K, Na, Ca, Mg and Fe. In turn, the highest (P < 0.05) concentrations of Zn, Se and Mn were determined in yolks of the eggs produced in the homestead system, and that of Cu – in yolks of the eggs from large-scale commercial production. A highly positive correlation between concentrations of minerals in the albumen and yolk of the analyzed eggs was reported for the following pairs: K-Na, Se (0.998, 0.93); Na-Na (0.949); Ca-Mg, Mn (0.994, 0.951); Mg-Ca, Mn (0.986, 0.982); P-Ca, Mn (0.997, 0.961); Fe-K, Mg, P, Zn (0.999, 0.937, 0.988, 0.999); Zn-Ca, Mn (0.999, 0.945); Se-Cu (0.971) and Mn-Ca (0.902), whereas a negative correlation for: Ca-Fe, Cu (-0.974, -0.994); Mg-Cu (-0.921); P-Fe (-0.933); Zn-Fe (-0.912); Cu-Na (-0.951); Se-K, P, Zn (-0.960, -0.910, -0.962) and Mn-Se (-0.979).
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