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1

Cadmium accumulation and its effects on growth and gas exchange in four populus cultivars

100%

Gu J., Qi L., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2007

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tom 49

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nr 2

7-14

2

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Effects of cadmium stress on root tip cells and some physiological indexes in Allium cepa var. agrogarum L.

100%

Zou J., Yue J., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2012

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tom 54

|

nr 1

Allium cepa var. agrogarum L. seedlings grown in nutrient solution were subjected to increasing concentrations of Cd2+ (0, 1, 10, 100 μM). Variation in tolerance to cadmium toxicity was studied based on chromosome aberrations, nucleoli structure and reconstruction of root tip cells, Cd accumulation and mineral metabolism, lipid peroxidation, and changes in the antioxidative defense system (SOD, CAT, POD) in leaves and roots of the seedlings. Cd induced chromosome aberrations including C-mitoses, chromosome bridges, chromosome fragments and chromosome stickiness. Cd induced the production of some particles of argyrophilic proteins scattered in the nuclei and even extruded from the nucleoli into the cytoplasm after a high Cd concentration or prolonged Cd stress, and nucleolar reconstruction was inhibited. In Cd2+-treated Allium cepa var. agrogarum plants the metal was largely restricted to the roots; very little of it was transported to aerial parts. Adding Cd2+ to the nutrient solution affected mineral metabolism. For example, at 100 μM Cd it reduced the levels of Mn, Cu and Zn in roots, bulbs and leaves. Malondialdehyde content in roots and leaves increased with treatment time and increased concentration of Cd. Antioxidant enzymes appear to play a key role in resistance to Cd under stress conditions.

3

Chromium accumulation and its effects on other mineral elements in Amaranthus viridis L.

100%

Zou J., Wang M., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2006

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tom 48

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nr 1

Chromium accumulation and its effects on other mineral elements in Amaranthus viridis L. were investigated using inductively coupled plasma atomic emission spectrometry (ICP-AES) and resin adsorption. The aim was to understand why A. viridis can grow well in soils heavily contaminated by Cr, what the forms of Cr in soils and residues are, and what effects Cr has on the distribution of Mn. Fe, Cu and Zn in the plant. The results indicated that A. viridis is not a hyperaccumulator, although it can grow well in soil containing a high concentration of Cr. The Cr concentration in plant tissues from a contaminated site was about 11 times higher than in those from an uncontaminated site. At both the contaminated and uncontaminated sites, Cr was accumulated primarily in its shoots, and in roots in much lower concentrations. The levels of Cr in A. viridis tissues were as follows: leaf > root > stem. Cr occurred predominantly as Cr(III). There was very little Cr(VI) in the polluted soil where A. viridis grew close to a waste heap discarded by a chromium(VI) production factory. The possible mechanisms for Cr accumulation of A. viridis and the effects of Cr on uptake and accumulation of the other mineral elements in A. viridis are briefly discussed.

4

Particleboards with partially liquefied bark of different particle sizes

88%

Jiang W., Adamopoulos S., Petric M., Sernek M., Medved S.

Drewno. Prace Naukowe. Doniesienia. Komunikaty

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2021

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tom 64

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nr 207

5

Effect of Cu2plus concentration on growth, antioxidant enzyme activity and malondialdehyde content in garlic [Allium sativum L.]

88%

Meng Q., Zou J., Zou J., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2007

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tom 49

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nr 1

95-101

The effects of different concentrations (10-5 M, 10-4 M. 10-3 M) of Cu2+ on growth, antioxidant enzyme activity and malondialdehyde (MDA) content were investigated in hydroponically grown Allium sativum L. The results indicated that the growth of garlic seedlings was not inhibited under treatment with 10-5 M Cu2+. Garlic seedlings exposed to 10-4 M and 10-3 M Cu2+ exhibited significant growth reduction. With increasing Cu2+ concentration and treatment time, superoxide dismutase (SOD) activity increased in leaves and roots, and peroxidase (POD) activity increased in leaves. In roots of plants exposed to 10-4 M and 10-3 M Cu2+, POD activity increased within 9 d and then dropped, but was still higher than in the control at the end of the experiment. Catalase (CAT) activity increased in seedlings grown at 10-5 M and l0-4 M, whereas a highly toxic level of Cu2+ (10-3 M) markedly inhibited CAT activity. SOD and POD activity were higher in roots than in leaves, whereas CAT activity was higher in leaves than in roots under both control and Cu2+ treatments. There was no obvious effect on MDA content in the seedlings treated with 10-5 M Cu2+; at 10-4 M and 10-3 M Cu2+ it increased. The mechanisms of Cu2+ toxicity and Cu2+ tolerance in garlic are briefly discussed.

6

Antioxidant response system and chlorophyll fluorescence in chromium [VI] - treated Zea mays L. seedlings

88%

Zou J., Yu K., Zhang Z., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2009

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tom 51

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nr 1

23-33

The effects of different concentrations of Cr(VI) (1 µM, 10 µM, 100 µM) applied for 7, 14 or 21 days on initiation of high lipid peroxidation level (POL) and consequent changes in the enzymatic-antioxidant protective system and minimization of photosystem II (PSII) activity were studied in maize seedlings. Chromium (VI) caused an increase in the electrical conductivity of the cell membrane, and malondialdehyde (MDA) content (a peroxidation product) reflected peroxidation of membrane lipids leading to the loss of the membrane's selective permeability. It also induced distinct and significant changes in antioxidant enzyme activity. Versus the control, superoxide dismutase (SOD, EC 1.15.1.1.), catalase (CAT, EC 1.11.1.6.) and peroxidase (POD, EC 1.11.1.11.) activity in maize seedling roots and leaves was progressively enhanced by the different Cr(VI) doses and stress periods, except for decreases in SOD and POD activity in leaves exposed to 100 µM Cr(VI) for 21 days. The different Cr(VI) concentrations changed chlorophyll (chl) content differently. The 10 µM and 100 µM doses of Cr(VI) decreased the chl a/b ratio and quenched the chl a fluorescence emission spectra. These effects reflect disturbance of the structure, composition and function of the photosynthetic apparatus as well as PSII activity.

7

Ultrastructural and photosynthetic response of Populus 107 leaves to cadmium stress

88%

Ge W., Jiao Y., Zou J., Jiang W., Liu D.

Polish Journal of Environmental Studies

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2015

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tom 24

|

nr 2

As Populus 107 is an efficient phytoextraction plant, it was used in the present investigation in order to better understand the mechanisms of detoxification and tolerance of Cd. Cd-induced impacts on photosynthetic parameters (chlorophyll content, soluble protein, and chlorophyll fluorescence) and ultrastructural changes in leaves of Populus 107 exposed to 50 μM, 100 μM, and 500 μM for 40 days were carried out. The results showed that in the Cd-treated cells, almost all the chloroplasts seemed to be affected. Cd induced several significant ultrastructural changes, including swollen chloroplast thylakoids, dissolved thylakoid grana, disintegrated chloroplasts, and numerous plastoglobuli in chloroplasts. Data from chlorophyll fluorescence showed that Fv/Fm, Fv ’/Fm’, ΦPSII, ETR, and qP decreased while qN increased in leaves of Populus 107 exposed to Cd when compared to control. The content of soluble protein increased with increasing Cd concentration and declined with prolonged duration of treatment. The soluble protein content in leaves treated with 50 μM Cd reached the maximum, which was 14.29% more than that of control. The content in leaves exposed to 500 μM Cd were only 61.76% of control. At the end of the experiment the contents of chlorophyll a, b, and a+b of Populus 107 treated with 500 μM Cd decreased to the minimum, which were 47.69%, 37.10%, and 45.49% of control, and respectively, and significantly (P < 0.05) lower than control. The toxic mechanisms of Cd are briefly explained.

8

An efficient regeneration system via direct and indirect organogenesis for the medicinal plant Dysosma versipellis (Hance) M.Cheng and its potential as a podophyllotoxin source

76%

Jiang W., Chen L., Pan Q., Qiu Y., Shen Y., Fu C.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 2

Dysosma versipellis (Hance) M. Cheng is an endangered plant due to overharvesting for the extraction of podophyllotoxin. Thus, the in vitro technique is valuable for the propagation of this species. When the explants of rhizome buds were cultured on Murashige and Skoog’s (MS) medium with 6-benzyladenine (BA) (1.0 mg l⁻¹), gibberellic acid (GA₃) (0.5 mg l⁻¹) and zeatin (Zea) (0.5 mg l⁻¹), multiple buds were regenerated directly on the explants without callusing within 6 weeks. Callus was induced from the leaf segment cultures on MS basal medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5 mg l⁻¹) and BA (0.2 mg l⁻¹) within 4 weeks. The adventitious buds were differentiated when the calli were subcultured on MS medium supplemented with BA (1.0 mg l⁻¹) and thidiazuron (TDZ) (0.2 mg l⁻¹) within 6 weeks. The adventitious buds obtained from callus and the rhizome-buds rooted with a frequency of 100% on half strength MS medium fortified with indole-3-butyric acid (IBA) 0.5 mg l⁻¹ and activated charcoal (AC) 0.5 g l⁻¹ for 4 weeks. The rooted shoots were successfully transplanted from a mixture of vermiculite:soil (1:1 v/v) to the field with a survival rate of 85%. Podophyllotoxin production in calli, cultured rhizomes, rhizomes of transplanting plants from the garden and rhizomes in the wild field was confirmed by high-performance liquid chromatography (HPLC) analysis. Our results suggest that calli, cultured rhizomes and rhizomes of transplanting plants would be the potential sources of podophyllotoxin.

9

Protein interactions of SOC1 with SVP are regulated by a few crucial amino acids in flowering pathways of Brassica juncea

76%

Li C., Gu H., Jiang W., Zou C., Wei D., Wang Z., Tang Q.

Acta Physiologiae Plantarum

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2019

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tom 41

|

nr 04

SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) is a vital flowering signal integrator to promote flowering, which is inhibited by a MADS-box transcription factor, SHORT VEGETATIVE PHASE (SVP). However, it remains elusive about how SOC1 interacts with SVP in flowering pathways of Brassica juncea. Here, B. juncea SOC1 (BjuSOC1) gene was cloned and it expressed differently between stem apexes and leaves during the low-temperature vernalization and long-day photoperiod pathways. Yeast two-hybrid and BiFC assays indicated that BjuSOC1 directly interacted with BjuSVP in vitro and in vivo. Interestingly, further studies indicated that mutants of BjuSOC1K108V, BjuSOC1R109L, BjuSOC1C137K could no more interact with BjuSVP, and BjuSVPR137L also led to loss of the protein interaction. It suggested that the 108th, 109th, and 137th of BjuSOC1 and 137th of BjuSVP regulated the protein interactions between BjuSOC1 and BjuSVP. The results provided valuable information for further study on the control of flowering time in B. juncea.

10

Effect of hydroxyl radical on the scission of cellular wall polysaccharides in vitro of banana fruit at various ripening stages

76%

Cheng G., Duan X., Yang B., Jiang Y., Lu W., Luo Y., Jiang W.

Acta Physiologiae Plantarum

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2008

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tom 30

|

nr 2

Fruit ripening is generally attributed to disassembly of cellular wall, particularly due to solubilisation and depolymerisation of pectin and hemicellulose. Experiments were conducted to test effects of hydroxyl radicals (OH) on the scission of cellular wall polysaccharides from pulp tissues of banana fruit at different ripening stage. Cellular wall materials were isolated from pulp tissues of banana fruit at different ripening stages. Two pectic fractions, water soluble pectin (WSP) and acid soluble pectin (ASP), and two hemicellulosic fractions, 1 M KOH soluble hemicellulose (HC1) and 4 M KOH soluble hemicellulos (HC2), were obtained from the cellular wall materials from pulp tissues, respectively. Effects of OH induced by the Fenton reaction on the scission of pectin and hemicellulose in vitro were investigated. As fruit ripening progressed, the sugar components of the WSP, HC1 and HC2 attacked by OH showed obvious molecular-mass downshifts. Thus, OH caused the disassembly of polysaccharides (WSP, ASP, HC1 and HC2) from cellular walls of pulp tissues of banana fruit, demonstrated by the reduced molecular mass distribution. Moreover, OH production in pulp tissues increased significantly as banana fruit ripened, which further help account for the role of OH in accelerated fruit ripening.

11

Effects of aluminum on nucleoli in root tip cells, root growth and the antioxidant defense system in Vicia faba L.

76%

Zhang H., Zhang S., Meng Q., Zou J., Jiang W., Liu D.

Acta Biologica Cracoviensia. Series Botanica

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2009

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tom 51

|

nr 2

99-106

The effects of different concentrations of Al (10 μM, 50 μM, 100 μM) on nucleoli in root tip cells, root growth, antioxidant enzyme activity and malondialdehyde (MDA) content were investigated in hydroponically grown Vicia faba L. Aluminum significantly inhibited root growth of V. faba treated with 50 μM and 100 μM Al. In the nucleolus in root tip cells, some particulates containing argyrophilic proteins were extruded from the nucleus into the cytoplasm, and some were scattered in the nucleus after Al stress. Superoxide dismutase (SOD) activity in leaves and roots exposed to different concentrations of Al was mostly higher than in the control. Seedlings exposed to 100 μM Al showed significantly higher peroxidase (POD) activity in roots than in the control. POD activity increased much more in roots than in leaves. Catalase (CAT) activity was lower in roots than in leaves. Malondialdehyde (MDA) content in leaves and roots of plants exposed to 50 μM and 100 μM Al was significantly higher than in the other groups and the control at 6 to 9 days of treatment. These results suggest that alterations in nucleoli and altered antioxidant enzyme activity and MDA content in V. faba can serve as useful biomarkers for detection of Al toxicity. The mechanisms of Al toxicity and tolerance in V. faba are briefly discussed

12

In vitro cloning potential and phytochemical evaluations of aneuploid individuals produced from reciprocal crosses between diploid and triploid in Echinacea purpurea L.

76%

Li Q., Jiang W., Ren Y., Chen R., Li X., Yang Y., Wu H.

Acta Societatis Botanicorum Poloniae

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2017

|

tom 86

|

nr 3

Aneuploidy often presents large variations in morphology, physiology, biochemistry, and genetics owing to karyotypic imbalance. This study aimed to evaluate the efficacy of aneuploid breeding in Echinacea purpurea L, an important medicinal plant. Reciprocal crosses between diploid and triploid plants were performed to generate aneuploid plants. Cross with triploid as female parent resulted in increased production of aneuploid individuals (19 of 23; 82.61%), while using diploid as female parent yielded much higher percentage of diploid progenies (130 of 133; 97.74%). Each aneuploid had particular karyotypic characteristics compared to the parents. The proportions of median, submedian, and subterminal centromere location chromosomes in gross chromosomes among aneuploids and two parents showed large variations. Although aneuploids had relatively lower adventitious bud regeneration rates than their parents, almost half of them looked morphologically normal, with high survival rates when transplanted to ex vitro conditions. Among the bioactive compounds assessed, cichoric acid and chlorogenic acid contents were extremely encouraging. Most aneuploids had higher cichoric acid and chlorogenic acid contents than their parents. For example, A2 had the highest cichoric acid content of 21.98 mg/g dry weight, more than twice the values of diploid and triploid. Meanwhile, A21 had the highest chlorogenic acid content of 1.84 mg/g, approximately five times more than the parental values. Eleven superior aneuploid lines were successfully screened as breeding candidates. The present findings indicated E. purpurea is highly tolerant of karyotypic imbalance and aneuploid plants could serve as prospective breeding resources in E. purpurea.

13

Characterization and expression analysis of the maize RING-H12 finger protein gene ZmXERICO responsive to plant hormones and abiotic stresses

76%

Gao Y., Li H., Deng D., Chen S., Jiang W., Chen J.

Acta Physiologiae Plantarum

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2012

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tom 34

|

nr 4

Really interesting new gene (RING)-H2 proteins are widely represented in plants and play important roles in the regulation of many developmental processes as well as in plant–environment interactions. In the present report, a full-length cDNA encoding a putative RING-H2 protein with high sequence homology to Arabidopsis thaliana RING-H2 gene XERICO, designated as ZmXERICO, was isolated from maize. The full-length cDNA of ZmXERICO contained a 435 bp open reading frame encoding a precursor protein of 145 amino acid residues. Comparative and bioinformatics analyses revealed that ZmXERICO showed a high degree of homology with RING-H2 proteins and contained the transmembrane domain (TM) and RING-H2 motif. In our study, ZmXERICO was analyzed for expression profiles in various organs (root, stem, leaf, stamen, pistil and seed) and responding to exogenous hormone treatment and abiotic stresses using real-time PCR. The results showed that the expression of ZmXERICO was different regulated after treated by gibberellins acid-3 (GA₃) and GA biosynthetic inhibitor paclobutrazol between in light and darkness. Moreover, the expression of ZmXERICO under different abiotic stresses was analyzed at different time points. The ZmXERICO was up-regulated in salt, drought, abscisic acid and cold stress. These expression results suggested that ZmXERICO played an important role in plant hormones signal pathways and light signal pathways, and that ZmXERICO might be involved in plant defense system against environmental stresses in maize.

14

Salix matsudana Koidz tolerance mechanisms to cadmium: uptake and accumulation, subcellular distribution, and chemical forms

64%

Wu H., Wang J., Li B., Ou Y., Wang J., Shi Q., Jiang W., Liu D., Zou J.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 4

Salix matsudana roots exposed to 10, 50, and 100 μM Cd solutions for 24 h were carried out in order to understand the mechanisms involved in Cd tolerance and detoxification. 50 and 100 μM Cd inhibited root length significantly (P < 0.05). Cd levels in roots increased significantly with increasing Cd concentrations, and the contents of Fe, Mn, Zn, and Ca decreased significantly. A Cd-specific Leadmium Green AM dye probe showed that the meristem zone was the absorption and accumulation site of Cd in the roots. Subcellular fractionation of Cd-containing tissues indicated that about 53% of the Cd was accumulated in the cell wall of S. matsudana roots at 10 μM Cd and 65% of the Cd at 100 μM Cd, indicating that Cd binding and/or precipitation in the cell wall in roots may serve as the first barrier to reduce the cytosolic-free Cd ions. The proportion of CdE and Cdw in roots is low when compared with the other Cd chemical forms. CdHCl, Cdr, and CdHAc represent 46% (10 μM Cd), 49% (50 μM Cd), and 59% (100 μM Cd) of total Cd, and CdNaCl represents 42% (10 μM Cd), 44% (50 μM Cd), and 32% (100 μM Cd).

15

Cd subcellular localization in root tips of Hordeum vulgare

64%

Shi Q., Wang J., Zou J., Jiang Z., Wang J., Wu H., Jiang W., Liu D.

Polish Journal of Environmental Studies

|

2016

|

tom 25

|

nr 2

The effects of Cd on the subcellular localization in root tip cells of Hordeum vulgare were investigated by Energy dispersive x-ray analysis (EDXA) in order to further understand Cd toxic mechanisms in plants. EDXA showed that Cd ions were localized in meristem, elongation, and mature zone in the root tips. In transverse section of the mature zone, Cd was accumulated in epidermal, cortical, and vessel cells, and the level of Cd is in the order: epidermal cells < vessel cells < cortical cells. In cortical cells Cd ions were observed in cytoplasm and walls.

Ograniczanie wyników

Cadmium accumulation and its effects on growth and gas exchange in four populus cultivars

Effects of cadmium stress on root tip cells and some physiological indexes in Allium cepa var. agrogarum L.

Chromium accumulation and its effects on other mineral elements in Amaranthus viridis L.

Particleboards with partially liquefied bark of different particle sizes

Effect of Cu2plus concentration on growth, antioxidant enzyme activity and malondialdehyde content in garlic [Allium sativum L.]

Antioxidant response system and chlorophyll fluorescence in chromium [VI] - treated Zea mays L. seedlings

Ultrastructural and photosynthetic response of Populus 107 leaves to cadmium stress

An efficient regeneration system via direct and indirect organogenesis for the medicinal plant Dysosma versipellis (Hance) M.Cheng and its potential as a podophyllotoxin source

Protein interactions of SOC1 with SVP are regulated by a few crucial amino acids in flowering pathways of Brassica juncea

Effect of hydroxyl radical on the scission of cellular wall polysaccharides in vitro of banana fruit at various ripening stages

Effects of aluminum on nucleoli in root tip cells, root growth and the antioxidant defense system in Vicia faba L.

In vitro cloning potential and phytochemical evaluations of aneuploid individuals produced from reciprocal crosses between diploid and triploid in Echinacea purpurea L.

Characterization and expression analysis of the maize RING-H12 finger protein gene ZmXERICO responsive to plant hormones and abiotic stresses

Salix matsudana Koidz tolerance mechanisms to cadmium: uptake and accumulation, subcellular distribution, and chemical forms

Cd subcellular localization in root tips of Hordeum vulgare