An efficient plant micropropagation system was established using shoot tips and stem segments of Lithospermum canescens and Onosma paniculatum (Boraginaceae). A high frequency of axillary shoot formation was achieved on LS [15] medium with 0.5 mg/1 kinetin and 2.0 mg/1 BAP (average shoot formation per explant was 6.18 ± 1.89 for L. canescens and 3.01 ± 0.82 for 0. paniculatum). The best results were obtained with 1x8 clone of L canescens. Axillary branching and root formation were the highest (8.22 shoot formation, 46.7% of rooting). Contamination of the explants with Rhodococcus fascians D188 increased the number of newly formed shoots of 0. paniculatum to 4.6 ± 0.72, while the propagation process of L. canescens was not affected. The highest percentage of rooted shoots of L canescens was observed on V, B5 medium [14] with 0.2 mg/1 1BA (from 27% to 46.7%, depending on plant line), while in the case of regenerated shoots of 0. paniculatum hormone-free LS medium induced roots more effectively (36.7%) than V2 B5 medium with 0.2 mg/1 1BA (25%).